ID   NAS2_HORVU              Reviewed;         335 AA.
AC   Q9ZQV7;
DT   13-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Probable nicotianamine synthase 2;
DE            EC=2.5.1.43;
DE   AltName: Full=HvNAS2;
DE   AltName: Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 2;
GN   Name=NAS2;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Ehimehadaka No.1; TISSUE=Root;
RX   PubMed=9952442; DOI=10.1104/pp.119.2.471;
RA   Higuchi K., Suzuki K., Nakanishi H., Yamaguchi H., Nishizawa N.-K.,
RA   Mori S.;
RT   "Cloning of nicotianamine synthase genes, novel genes involved in the
RT   biosynthesis of phytosiderophores.";
RL   Plant Physiol. 119:471-480(1999).
CC   -!- FUNCTION: Synthesizes nicotianamine, a polyamine that is the first
CC       intermediate in the synthesis of the phytosiderophores of the mugineic
CC       acid type found in gramineae which serves as a sensor for the
CC       physiological iron status within the plant, and/or might be involved in
CC       the transport of iron. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 S-adenosyl-L-methionine = 3 H(+) + nicotianamine + 3 S-
CC         methyl-5'-thioadenosine; Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC   -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC       {ECO:0000305}.
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DR   EMBL; AB011265; BAA74582.1; -; mRNA.
DR   AlphaFoldDB; Q9ZQV7; -.
DR   SMR; Q9ZQV7; -.
DR   BRENDA; 2.5.1.43; 2687.
DR   ExpressionAtlas; Q9ZQV7; baseline.
DR   GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR004298; Nicotian_synth.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR32266; PTHR32266; 1.
DR   Pfam; PF03059; NAS; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51142; NAS; 1.
PE   2: Evidence at transcript level;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..335
FT                   /note="Probable nicotianamine synthase 2"
FT                   /id="PRO_0000212705"
SQ   SEQUENCE   335 AA;  35841 MW;  DAF72C26622C824B CRC64;
     MAAQNNQEVD ALVEKITGLH AAIAKLPSLS PSPDVDALFT ELVTACVPPS PVDVTKLGPE
     AQEMREGLIR LCSEAEGKLE AHYSDMLAAF DKPLDHLGMF PYYNNYINLS KLEYELLARY
     VPGGYRPARV AFIGSGPLPF SSFVLAARHL PDTMFDNYDL CGAANDRASK LFRADRDVGA
     RMSFHTADVA DLAGELAKYD VVFLAALVGM AAEDKAKVIA HLGAHMADGA ALVVRSAHGA
     RGFLYPIVDP QDIGRGGFEV LAVCHPDDDV VNSVIIAQKS KDVHADGLGS GRGAGGQYAR
     GTVPVVSPPC RFGEMVADVT QNHKRDEFAN AEVAF