ID   NAS2_ORYSI              Reviewed;         326 AA.
AC   A2XFU5; Q9FEG8;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   03-AUG-2022, entry version 51.
DE   RecName: Full=Nicotianamine synthase 2;
DE            EC=2.5.1.43;
DE   AltName: Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 2;
DE            Short=OsNAS2;
GN   Name=NAS2; ORFNames=OsI_010938;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=cv. IR36; TISSUE=Root;
RX   PubMed=11169192; DOI=10.1046/j.1365-313x.2001.00951.x;
RA   Higuchi K., Watanabe S., Takahashi M., Kawasaki S., Nakanishi H.,
RA   Nishizawa N.-K., Mori S.;
RT   "Nicotianamine synthase gene expression differs in barley and rice under
RT   Fe-deficient conditions.";
RL   Plant J. 25:159-167(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [3]
RP   FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=14617093; DOI=10.1046/j.1365-313x.2003.01878.x;
RA   Inoue H., Higuchi K., Takahashi M., Nakanishi H., Mori S., Nishizawa N.K.;
RT   "Three rice nicotianamine synthase genes, OsNAS1, OsNAS2, and OsNAS3 are
RT   expressed in cells involved in long-distance transport of iron and
RT   differentially regulated by iron.";
RL   Plant J. 36:366-381(2003).
CC   -!- FUNCTION: Synthesizes nicotianamine, a polyamine that is the first
CC       intermediate in the synthesis of the phytosiderophores of the mugineic
CC       acid type found in gramineae which serve as a sensor for the
CC       physiological iron status within the plant, and/or might be involved in
CC       the transport of iron. {ECO:0000269|PubMed:14617093}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 S-adenosyl-L-methionine = 3 H(+) + nicotianamine + 3 S-
CC         methyl-5'-thioadenosine; Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC   -!- TISSUE SPECIFICITY: Expressed in roots. {ECO:0000269|PubMed:14617093}.
CC   -!- INDUCTION: By iron deficiency in roots and chlorotic leaves.
CC       {ECO:0000269|PubMed:14617093}.
CC   -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB023818; BAB17823.1; -; mRNA.
DR   EMBL; AB046401; BAB17826.1; -; Genomic_DNA.
DR   EMBL; CM000128; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; A2XFU5; -.
DR   SMR; A2XFU5; -.
DR   STRING; 39946.A2XFU5; -.
DR   BioCyc; MetaCyc:MON-13937; -.
DR   BRENDA; 2.5.1.43; 11590.
DR   Proteomes; UP000007015; Chromosome 3.
DR   GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR004298; Nicotian_synth.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR32266; PTHR32266; 2.
DR   Pfam; PF03059; NAS; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51142; NAS; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..326
FT                   /note="Nicotianamine synthase 2"
FT                   /id="PRO_0000300239"
FT   CONFLICT        234..235
FT                   /note="SA -> R (in Ref. 1; BAB17823/BAB17826)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   326 AA;  34456 MW;  0B9D09E2888A659C CRC64;
     MEAQNQEVAA LVEKIAGLHA AISKLPSLSP SAEVDALFTD LVTACVPASP VDVAKLGPEA
     QAMREELIRL CSAAEGHLEA HYADMLAAFD NPLDHLARFP YYGNYVNLSK LEYDLLVRYV
     PGIAPTRVAF VGSGPLPFSS LVLAAHHLPD AVFDNYDRCG AANERARRLF RGADEGLGAR
     MAFHTADVAT LTGELGAYDV VFLAALVGMA AEEKAGVIAH LGAHMADGAA LVVSARHGAR
     GFLYPIVDLE DIRRGGFDVL AVYHPDDEVI NSVIVARKAD PRRGGGLAGA RGAVPVVSPP
     CKCCKMEAAA GAFQKAEEFA AKRLSV