NAS2_ORYSJ
ID NAS2_ORYSJ Reviewed; 326 AA.
AC Q10MI9; A0A0P0VWJ2; A3AH72; Q9FEG8;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-MAY-2016, sequence version 2.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Nicotianamine synthase 2;
DE EC=2.5.1.43;
DE AltName: Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 2;
DE Short=OsNAS2;
GN Name=NAS2;
GN OrderedLocusNames=Os03g0307200 {ECO:0000312|EMBL:BAS83821.1},
GN LOC_Os03g19420 {ECO:0000305}; ORFNames=OsJ_010144;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=14617093; DOI=10.1046/j.1365-313x.2003.01878.x;
RA Inoue H., Higuchi K., Takahashi M., Nakanishi H., Mori S., Nishizawa N.K.;
RT "Three rice nicotianamine synthase genes, OsNAS1, OsNAS2, and OsNAS3 are
RT expressed in cells involved in long-distance transport of iron and
RT differentially regulated by iron.";
RL Plant J. 36:366-381(2003).
CC -!- FUNCTION: Synthesizes nicotianamine, a polyamine that is the first
CC intermediate in the synthesis of the phytosiderophores of the mugineic
CC acid type found in gramineae which serve as a sensor for the
CC physiological iron status within the plant, and/or might be involved in
CC the transport of iron. {ECO:0000269|PubMed:14617093}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 S-adenosyl-L-methionine = 3 H(+) + nicotianamine + 3 S-
CC methyl-5'-thioadenosine; Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC -!- TISSUE SPECIFICITY: Expressed in roots. {ECO:0000269|PubMed:14617093}.
CC -!- INDUCTION: By iron deficiency in roots and chlorotic leaves.
CC {ECO:0000269|PubMed:14617093}.
CC -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC {ECO:0000305}.
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DR EMBL; DP000009; ABF95537.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF11808.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS83821.1; -; Genomic_DNA.
DR EMBL; CM000140; EAZ26661.1; -; Genomic_DNA.
DR EMBL; AK112011; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015630628.1; XM_015775142.1.
DR AlphaFoldDB; Q10MI9; -.
DR SMR; Q10MI9; -.
DR STRING; 4530.OS03T0307200-01; -.
DR PaxDb; Q10MI9; -.
DR PRIDE; Q10MI9; -.
DR EnsemblPlants; Os03t0307200-01; Os03t0307200-01; Os03g0307200.
DR GeneID; 4332607; -.
DR Gramene; Os03t0307200-01; Os03t0307200-01; Os03g0307200.
DR KEGG; osa:4332607; -.
DR eggNOG; ENOG502QTU6; Eukaryota.
DR HOGENOM; CLU_031919_0_0_1; -.
DR InParanoid; Q10MI9; -.
DR OMA; TIFHNFD; -.
DR OrthoDB; 1009301at2759; -.
DR BRENDA; 2.5.1.43; 4460.
DR PlantReactome; R-OSA-9025754; Mugineic acid biosynthesis.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q10MI9; OS.
DR GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR004298; Nicotian_synth.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR32266; PTHR32266; 2.
DR Pfam; PF03059; NAS; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS51142; NAS; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..326
FT /note="Nicotianamine synthase 2"
FT /id="PRO_0000212715"
FT CONFLICT 9
FT /note="A -> T (in Ref. 1; ABF95537 and 2; BAF11808)"
SQ SEQUENCE 326 AA; 34456 MW; 3C02D162A9B966AC CRC64;
MEAQNQEVAA LVEKIAGLHA AISKLPSLSP SAEVDALFTD LVTACVPASP VDVAKLGPEA
QAMREELIRL CSAAEGHLEA HYADMLAAFD NPLDHLARFP YYGNYVNLSK LEYDLLVRYV
PGIAPTRVAF VGSGPLPFSS LVLAAHHLPD AVFDNYDRCG AANERARRLF RGADEGLGAR
MAFHTADVAT LTGELGAYDV VFLAALVGMA AEEKAGVIAH LGAHMADGAA LVVRSAHGAR
GFLYPIVDLE DIRRGGFDVL AVYHPDDEVI NSVIVARKAD PRRGGGLAGA RGAVPVVSPP
CKCCKMEAAA GAFQKAEEFA AKRLSV
