ID   NAS3_ORYSI              Reviewed;         343 AA.
AC   A2YQ58; Q69UZ9; Q9FXW5;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Nicotianamine synthase 3;
DE            EC=2.5.1.43;
DE   AltName: Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 3;
DE            Short=OsNAS3;
GN   Name=NAS3; ORFNames=OsI_026451;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. IR36; TISSUE=Root;
RX   PubMed=11169192; DOI=10.1046/j.1365-313x.2001.00951.x;
RA   Higuchi K., Watanabe S., Takahashi M., Kawasaki S., Nakanishi H.,
RA   Nishizawa N.-K., Mori S.;
RT   "Nicotianamine synthase gene expression differs in barley and rice under
RT   Fe-deficient conditions.";
RL   Plant J. 25:159-167(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [3]
RP   FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=14617093; DOI=10.1046/j.1365-313x.2003.01878.x;
RA   Inoue H., Higuchi K., Takahashi M., Nakanishi H., Mori S., Nishizawa N.K.;
RT   "Three rice nicotianamine synthase genes, OsNAS1, OsNAS2, and OsNAS3 are
RT   expressed in cells involved in long-distance transport of iron and
RT   differentially regulated by iron.";
RL   Plant J. 36:366-381(2003).
CC   -!- FUNCTION: Synthesizes nicotianamine, a polyamine that is the first
CC       intermediate in the synthesis of the phytosiderophores of the mugineic
CC       acid type found in gramineae which serve as a sensor for the
CC       physiological iron status within the plant, and/or might be involved in
CC       the transport of iron. {ECO:0000269|PubMed:14617093}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 S-adenosyl-L-methionine = 3 H(+) + nicotianamine + 3 S-
CC         methyl-5'-thioadenosine; Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC   -!- TISSUE SPECIFICITY: Expressed in leaves. {ECO:0000269|PubMed:14617093}.
CC   -!- INDUCTION: By iron deficiency in roots. Down-regulated in chlorotic
CC       leaves by iron deficiency. {ECO:0000269|PubMed:14617093}.
CC   -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAZ05219.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AB023819; BAB17824.1; -; mRNA.
DR   EMBL; CM000132; EAZ05219.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; A2YQ58; -.
DR   SMR; A2YQ58; -.
DR   STRING; 39946.A2YQ58; -.
DR   HOGENOM; CLU_031919_0_0_1; -.
DR   BioCyc; MetaCyc:MON-13939; -.
DR   BRENDA; 2.5.1.43; 11590.
DR   Proteomes; UP000007015; Chromosome 7.
DR   GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR004298; Nicotian_synth.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR32266; PTHR32266; 2.
DR   Pfam; PF03059; NAS; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51142; NAS; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..343
FT                   /note="Nicotianamine synthase 3"
FT                   /id="PRO_0000300240"
FT   CONFLICT        249
FT                   /note="S -> T (in Ref. 1; BAB17824)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        292..297
FT                   /note="KPPVAA -> NRPWPG (in Ref. 1; BAB17824)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   343 AA;  36983 MW;  1D86A2AEFB0B93EA CRC64;
     MTVEVEAVTM AKEEQPEEEE VIEKLVEKIT GLAAAIGKLP SLSPSPEVNA LFTELVMTCI
     PPSSVDVEQL GAEAQDMRGR LIRLCADAEG HLEAHYSDVL AAHDNPLDHL ALFPYFNNYI
     QLAQLEYALL ARHLPAAPPP SRLAFLGSGP LPLSSLVLAA RHLPAASFHN YDICADANRR
     ASRLVRADRD LSARMAFHTS DVAHVTTDLA AYDVVFLAAL VGMAAEEKAR MVEHLGKHMA
     PGAALVVRSA HGARGFLYPV VDPEEIRRGG FDVLAVHHPE GEVINSVIIA RKPPVAAPAL
     EGGDAHAHGH GAVVSRPCQR CEMEARAHQK MEDMSAMEKL PSS