ID   NAS3_ORYSJ              Reviewed;         343 AA.
AC   Q0D3F2; A3BNM6; Q69UZ9; Q9FXW5;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Nicotianamine synthase 3;
DE            EC=2.5.1.43;
DE   AltName: Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 3;
DE            Short=OsNAS3;
GN   Name=NAS3; OrderedLocusNames=Os07g0689600, LOC_Os07g48980;
GN   ORFNames=OJ1165_F02.29, OsJ_024648, P0597G07.112;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=14617093; DOI=10.1046/j.1365-313x.2003.01878.x;
RA   Inoue H., Higuchi K., Takahashi M., Nakanishi H., Mori S., Nishizawa N.K.;
RT   "Three rice nicotianamine synthase genes, OsNAS1, OsNAS2, and OsNAS3 are
RT   expressed in cells involved in long-distance transport of iron and
RT   differentially regulated by iron.";
RL   Plant J. 36:366-381(2003).
CC   -!- FUNCTION: Synthesizes nicotianamine, a polyamine that is the first
CC       intermediate in the synthesis of the phytosiderophores of the mugineic
CC       acid type found in gramineae which serve as a sensor for the
CC       physiological iron status within the plant, and/or might be involved in
CC       the transport of iron. {ECO:0000269|PubMed:14617093}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 S-adenosyl-L-methionine = 3 H(+) + nicotianamine + 3 S-
CC         methyl-5'-thioadenosine; Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC   -!- TISSUE SPECIFICITY: Expressed in leaves. {ECO:0000269|PubMed:14617093}.
CC   -!- INDUCTION: By iron deficiency in roots. Down-regulated in chlorotic
CC       leaves by iron deficiency. {ECO:0000269|PubMed:14617093}.
CC   -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAZ41165.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AP003816; BAC21363.1; -; Genomic_DNA.
DR   EMBL; AP004316; BAD30599.1; -; Genomic_DNA.
DR   EMBL; AP008213; BAF22621.1; -; Genomic_DNA.
DR   EMBL; AP014963; BAT03321.1; -; Genomic_DNA.
DR   EMBL; CM000144; EAZ41165.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AK070656; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015645101.1; XM_015789615.1.
DR   AlphaFoldDB; Q0D3F2; -.
DR   SMR; Q0D3F2; -.
DR   STRING; 4530.OS07T0689600-01; -.
DR   PaxDb; Q0D3F2; -.
DR   PRIDE; Q0D3F2; -.
DR   EnsemblPlants; Os07t0689600-01; Os07t0689600-01; Os07g0689600.
DR   GeneID; 4344361; -.
DR   Gramene; Os07t0689600-01; Os07t0689600-01; Os07g0689600.
DR   KEGG; osa:4344361; -.
DR   eggNOG; ENOG502QTU6; Eukaryota.
DR   HOGENOM; CLU_031919_0_0_1; -.
DR   InParanoid; Q0D3F2; -.
DR   OMA; SERMFFH; -.
DR   OrthoDB; 1009301at2759; -.
DR   BRENDA; 2.5.1.43; 4460.
DR   PlantReactome; R-OSA-9025754; Mugineic acid biosynthesis.
DR   Proteomes; UP000000763; Chromosome 7.
DR   Proteomes; UP000007752; Chromosome 7.
DR   Proteomes; UP000059680; Chromosome 7.
DR   ExpressionAtlas; Q0D3F2; baseline and differential.
DR   Genevisible; Q0D3F2; OS.
DR   GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR004298; Nicotian_synth.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR32266; PTHR32266; 2.
DR   Pfam; PF03059; NAS; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51142; NAS; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..343
FT                   /note="Nicotianamine synthase 3"
FT                   /id="PRO_0000212716"
FT   CONFLICT        301
FT                   /note="E -> G (in Ref. 5; AK070656)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   343 AA;  36983 MW;  1D86A2AEFB0B93EA CRC64;
     MTVEVEAVTM AKEEQPEEEE VIEKLVEKIT GLAAAIGKLP SLSPSPEVNA LFTELVMTCI
     PPSSVDVEQL GAEAQDMRGR LIRLCADAEG HLEAHYSDVL AAHDNPLDHL ALFPYFNNYI
     QLAQLEYALL ARHLPAAPPP SRLAFLGSGP LPLSSLVLAA RHLPAASFHN YDICADANRR
     ASRLVRADRD LSARMAFHTS DVAHVTTDLA AYDVVFLAAL VGMAAEEKAR MVEHLGKHMA
     PGAALVVRSA HGARGFLYPV VDPEEIRRGG FDVLAVHHPE GEVINSVIIA RKPPVAAPAL
     EGGDAHAHGH GAVVSRPCQR CEMEARAHQK MEDMSAMEKL PSS