ID   NAS4_HORVU              Reviewed;         329 AA.
AC   Q9ZQV6;
DT   13-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Probable nicotianamine synthase 4;
DE            EC=2.5.1.43;
DE   AltName: Full=HvNAS4;
DE   AltName: Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 4;
GN   Name=NAS4;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Ehimehadaka No.1; TISSUE=Root;
RX   PubMed=9952442; DOI=10.1104/pp.119.2.471;
RA   Higuchi K., Suzuki K., Nakanishi H., Yamaguchi H., Nishizawa N.-K.,
RA   Mori S.;
RT   "Cloning of nicotianamine synthase genes, novel genes involved in the
RT   biosynthesis of phytosiderophores.";
RL   Plant Physiol. 119:471-480(1999).
CC   -!- FUNCTION: Synthesizes nicotianamine, a polyamine that is the first
CC       intermediate in the synthesis of the phytosiderophores of the mugineic
CC       acid type found in gramineae which serves as a sensor for the
CC       physiological iron status within the plant, and/or might be involved in
CC       the transport of iron. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 S-adenosyl-L-methionine = 3 H(+) + nicotianamine + 3 S-
CC         methyl-5'-thioadenosine; Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC   -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC       {ECO:0000305}.
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DR   EMBL; AB011266; BAA74583.1; -; mRNA.
DR   AlphaFoldDB; Q9ZQV6; -.
DR   SMR; Q9ZQV6; -.
DR   BRENDA; 2.5.1.43; 2687.
DR   ExpressionAtlas; Q9ZQV6; baseline and differential.
DR   GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR004298; Nicotian_synth.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR32266; PTHR32266; 1.
DR   Pfam; PF03059; NAS; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51142; NAS; 1.
PE   2: Evidence at transcript level;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..329
FT                   /note="Probable nicotianamine synthase 4"
FT                   /id="PRO_0000212707"
SQ   SEQUENCE   329 AA;  35398 MW;  7BD13522B308E259 CRC64;
     MDGQSEEVDA LVQKITGLHA AIAKLPSLSP SPDVDALFTD LVTACVPPSP VDVTKLAPEA
     QAMREGLIRL CSEAEGKLEA HYSDMLAAFD NPLDHLGVFP YYSNYINLSK LEYELLARYV
     PGRHRPARVA FIGSGPLPFS SYVLAARHLP DTVFDNYDLC GAANDRATRL FRADKDVGAR
     MSFHTADVAD LTDELATYDV VFLAALVGMA AEDKAKVIAH LGAHMADGAA LVARHGARGF
     LYPIVDPQDI GRGGFEVLAV CHPDDDVVNS VIIAQKSNDV HEYGLGSGRG GRYARGTVVP
     VVSPPCRFGE MVADVTQKRE EFANAEVAF