ID   NAS5_HORVU              Reviewed;         282 AA.
AC   Q9ZQV4; Q9ZQV5;
DT   13-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 51.
DE   RecName: Full=Nicotianamine synthase-like 5 protein;
DE   AltName: Full=HvNAS5;
GN   Name=NAS5;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 180-198 AND 224-252.
RC   STRAIN=cv. Ehimehadaka No.1; TISSUE=Root;
RX   PubMed=9952442; DOI=10.1104/pp.119.2.471;
RA   Higuchi K., Suzuki K., Nakanishi H., Yamaguchi H., Nishizawa N.-K.,
RA   Mori S.;
RT   "Cloning of nicotianamine synthase genes, novel genes involved in the
RT   biosynthesis of phytosiderophores.";
RL   Plant Physiol. 119:471-480(1999).
CC   -!- MISCELLANEOUS: Homologous to nicotianamine synthase, but without
CC       enzymatic activity.
CC   -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC       {ECO:0000305}.
CC   -!- CAUTION: Two forms of this protein have been cloned (NAS5-1 and NAS5-2)
CC       which seem to be identical with the exception of a 15 amino acids
CC       deletion. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA74584.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AB011268; BAA74585.1; -; mRNA.
DR   EMBL; AB011267; BAA74584.1; ALT_FRAME; mRNA.
DR   AlphaFoldDB; Q9ZQV4; -.
DR   SMR; Q9ZQV4; -.
DR   ExpressionAtlas; Q9ZQV4; baseline and differential.
DR   GO; GO:0030410; F:nicotianamine synthase activity; IEA:InterPro.
DR   GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR004298; Nicotian_synth.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR32266; PTHR32266; 1.
DR   Pfam; PF03059; NAS; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51142; NAS; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing.
FT   CHAIN           1..282
FT                   /note="Nicotianamine synthase-like 5 protein"
FT                   /id="PRO_0000212708"
FT   CONFLICT        120..135
FT                   /note="VAAPARVAFIGSGPLP -> W (in Ref. 1; BAA74584)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        230..234
FT                   /note="VREAL -> RARSA (in Ref. 1; BAA74584)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   282 AA;  30149 MW;  32D8608194C507D0 CRC64;
     MEAENGEVAA LVEKITGLHA AISKLPALSP SPQVDALFTE LVAACVPSSP VDVTKLGPEA
     QEMRQDLIRL CSAAEGLLEA HYSDMLTALD SPLDHLGRFP YFDNYVNLSK LEHDLLAGHV
     AAPARVAFIG SGPLPFSSLF LATYHLPDTR FDNYDRCSVA NGRAMKLVGA ADEGVRSRMA
     FHTAEVTDLT AELGAYDVVF LAALVGMTSK EKADAIAHLG KHMADGAVLV REALHGARAF
     LYPVVELDDV GRGGFQVLAV HHPAGDEVFN SFIVARKVKM SA