ID   NAS6_HORVU              Reviewed;         328 AA.
AC   Q9ZQV3;
DT   13-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Probable nicotianamine synthase 6;
DE            EC=2.5.1.43;
DE   AltName: Full=HvNAS6;
DE   AltName: Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 6;
GN   Name=NAS6;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Ehimehadaka No.1; TISSUE=Root;
RX   PubMed=9952442; DOI=10.1104/pp.119.2.471;
RA   Higuchi K., Suzuki K., Nakanishi H., Yamaguchi H., Nishizawa N.-K.,
RA   Mori S.;
RT   "Cloning of nicotianamine synthase genes, novel genes involved in the
RT   biosynthesis of phytosiderophores.";
RL   Plant Physiol. 119:471-480(1999).
CC   -!- FUNCTION: Synthesizes nicotianamine, a polyamine that is the first
CC       intermediate in the synthesis of the phytosiderophores of the mugineic
CC       acid type found in gramineae which serves as a sensor for the
CC       physiological iron status within the plant, and/or might be involved in
CC       the transport of iron. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 S-adenosyl-L-methionine = 3 H(+) + nicotianamine + 3 S-
CC         methyl-5'-thioadenosine; Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC   -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC       {ECO:0000305}.
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DR   EMBL; AB011269; BAA74586.1; -; mRNA.
DR   AlphaFoldDB; Q9ZQV3; -.
DR   SMR; Q9ZQV3; -.
DR   BRENDA; 2.5.1.43; 2687.
DR   ExpressionAtlas; Q9ZQV3; baseline.
DR   GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR004298; Nicotian_synth.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR32266; PTHR32266; 1.
DR   Pfam; PF03059; NAS; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51142; NAS; 1.
PE   2: Evidence at transcript level;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..328
FT                   /note="Probable nicotianamine synthase 6"
FT                   /id="PRO_0000212709"
SQ   SEQUENCE   328 AA;  35352 MW;  4F5D10C981DCA584 CRC64;
     MDAQNKEVDA LVQKITGLHA AIAKLPSLSP SPDVDALFTD LVTACVPPSP VDVTKLGSEA
     QEMREGLIRL CSEAEGKLEA HYSDMLAAFD NPLDHLGMFP YYSNYINLSK LEYELLARYV
     PGGIARPAVA FIGSGPLPFS SYVLAARHLP DAMFDNYDLC SAANDRASKL FRADKDVGAR
     MSFHTADVAD LTRELAAYDV VFLAALVGMA AEDKAKVIPH LGAHMADGAA LVVRSAQARG
     FLYPIVDPQD IGRGGFEVLA VCHPDDDVVN SVIIAHKSKD VHANERPNGR GGQYRGAVPV
     VSPPCRFGEM VADVTHKREE FTNAEVAF