ID   NAS7_HORVU              Reviewed;         329 AA.
AC   Q9ZWH8;
DT   13-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Probable nicotianamine synthase 7;
DE            EC=2.5.1.43;
DE   AltName: Full=HvNAS7;
DE   AltName: Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 7;
GN   Name=NAS7;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Ehimehadaka No.1; TISSUE=Root;
RA   Mori S., Higuchi K.;
RL   Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Synthesizes nicotianamine, a polyamine that is the first
CC       intermediate in the synthesis of the phytosiderophores of the mugineic
CC       acid type found in gramineae which serves as a sensor for the
CC       physiological iron status within the plant, and/or might be involved in
CC       the transport of iron. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 S-adenosyl-L-methionine = 3 H(+) + nicotianamine + 3 S-
CC         methyl-5'-thioadenosine; Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC   -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB019525; BAA74587.1; -; mRNA.
DR   AlphaFoldDB; Q9ZWH8; -.
DR   SMR; Q9ZWH8; -.
DR   ExpressionAtlas; Q9ZWH8; baseline and differential.
DR   GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR004298; Nicotian_synth.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR32266; PTHR32266; 1.
DR   Pfam; PF03059; NAS; 1.
DR   PROSITE; PS51142; NAS; 1.
PE   2: Evidence at transcript level;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..329
FT                   /note="Probable nicotianamine synthase 7"
FT                   /id="PRO_0000212710"
SQ   SEQUENCE   329 AA;  35246 MW;  8BD7E0E660F963B9 CRC64;
     MDAQSKEVDA LVQKITGLHA AIAKLPSLSP SPDVDALFTD LVTACVPPSP VDVTKLAPEA
     QAMREGLIRL CSEAEGKLEA HYSDMLAAFD NPLDHLGVFP YYSNYINLSK LEYELLARYV
     PGGIAPARVA FIGSGPLPFS SYVLAARHLP DTVFDNYVPV RAANDRATRL FRADKDVGAR
     MSFHTADVAD LTDELATYDV VFLAALVGMA AEDKGQGDPH LGAHMADGAA LVRSAHGARG
     FLYPIVDPQD IGRGGFEVLA VCHPDDDVVN SVIIAQKSKD MFANGPRNGC GGRYARGTVP
     VVSPPCRFGE MVADVTQKRE EFAKAEVAF