NAS9_CAEEL
ID NAS9_CAEEL Reviewed; 546 AA.
AC P91137; Q8I7H6; Q95Q69;
DT 04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Zinc metalloproteinase nas-9;
DE EC=3.4.24.- {ECO:0000250|UniProtKB:A8Q2D1};
DE AltName: Full=Nematode astacin 9;
DE Flags: Precursor;
GN Name=nas-9; ORFNames=C37H5.9;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP NOMENCLATURE, AND DISRUPTION PHENOTYPE.
RX PubMed=14653817; DOI=10.1046/j.1432-1033.2003.03891.x;
RA Moehrlen F., Hutter H., Zwilling R.;
RT "The astacin protein family in Caenorhabditis elegans.";
RL Eur. J. Biochem. 270:4909-4920(2003).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=20109220; DOI=10.1186/1471-213x-10-14;
RA Park J.O., Pan J., Moehrlen F., Schupp M.O., Johnsen R., Baillie D.L.,
RA Zapf R., Moerman D.G., Hutter H.;
RT "Characterization of the astacin family of metalloproteases in C.
RT elegans.";
RL BMC Dev. Biol. 10:14-14(2010).
CC -!- FUNCTION: Metalloprotease. {ECO:0000250|UniProtKB:P07584}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU01211};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000255|PROSITE-
CC ProRule:PRU01211};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=b;
CC IsoId=P91137-1; Sequence=Displayed;
CC Name=a;
CC IsoId=P91137-2; Sequence=VSP_012353;
CC Name=c;
CC IsoId=P91137-3; Sequence=VSP_012352;
CC -!- TISSUE SPECIFICITY: Expressed in hypodermis, uterus and spermatheca.
CC {ECO:0000269|PubMed:20109220}.
CC -!- DISRUPTION PHENOTYPE: Defects lead to embryonic lethality in 6% of
CC population. {ECO:0000269|PubMed:14653817}.
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DR EMBL; FO080813; CCD66988.1; -; Genomic_DNA.
DR EMBL; FO080813; CCD66989.1; -; Genomic_DNA.
DR EMBL; FO080813; CCD66990.1; -; Genomic_DNA.
DR PIR; T25615; T25615.
DR RefSeq; NP_504293.2; NM_071892.8. [P91137-3]
DR RefSeq; NP_741531.1; NM_171452.1. [P91137-2]
DR RefSeq; NP_741532.1; NM_171927.1. [P91137-1]
DR AlphaFoldDB; P91137; -.
DR SMR; P91137; -.
DR BioGRID; 43925; 1.
DR STRING; 6239.C37H5.9b; -.
DR MEROPS; M12.A22; -.
DR EPD; P91137; -.
DR PaxDb; P91137; -.
DR PeptideAtlas; P91137; -.
DR EnsemblMetazoa; C37H5.9a.1; C37H5.9a.1; WBGene00003528. [P91137-2]
DR EnsemblMetazoa; C37H5.9b.1; C37H5.9b.1; WBGene00003528. [P91137-1]
DR EnsemblMetazoa; C37H5.9c.1; C37H5.9c.1; WBGene00003528. [P91137-3]
DR EnsemblMetazoa; C37H5.9c.2; C37H5.9c.2; WBGene00003528. [P91137-3]
DR GeneID; 178875; -.
DR KEGG; cel:CELE_C37H5.9; -.
DR UCSC; C37H5.9c.1; c. elegans. [P91137-1]
DR CTD; 178875; -.
DR WormBase; C37H5.9a; CE08632; WBGene00003528; nas-9. [P91137-2]
DR WormBase; C37H5.9b; CE29710; WBGene00003528; nas-9. [P91137-1]
DR WormBase; C37H5.9c; CE32825; WBGene00003528; nas-9. [P91137-3]
DR eggNOG; KOG3714; Eukaryota.
DR GeneTree; ENSGT00970000196429; -.
DR InParanoid; P91137; -.
DR OMA; MHETMHA; -.
DR OrthoDB; 567013at2759; -.
DR PhylomeDB; P91137; -.
DR PRO; PR:P91137; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00003528; Expressed in larva and 2 other tissues.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004222; F:metalloendopeptidase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd04280; ZnMc_astacin_like; 1.
DR Gene3D; 3.40.390.10; -; 1.
DR InterPro; IPR034035; Astacin-like_dom.
DR InterPro; IPR024079; MetalloPept_cat_dom_sf.
DR InterPro; IPR001506; Peptidase_M12A.
DR InterPro; IPR017368; Peptidase_M12A_astacin-9/10/11.
DR InterPro; IPR006026; Peptidase_Metallo.
DR InterPro; IPR003582; ShKT_dom.
DR Pfam; PF01400; Astacin; 1.
DR Pfam; PF01549; ShK; 1.
DR PIRSF; PIRSF038055; Nas9/Nas10/Nas11; 1.
DR PRINTS; PR00480; ASTACIN.
DR SMART; SM00235; ZnMc; 1.
DR PROSITE; PS51864; ASTACIN; 1.
DR PROSITE; PS51670; SHKT; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Hydrolase;
KW Metal-binding; Metalloprotease; Protease; Reference proteome; Secreted;
KW Signal; Zinc; Zymogen.
FT SIGNAL 1..14
FT /evidence="ECO:0000255"
FT PROPEP 15..300
FT /evidence="ECO:0000250|UniProtKB:P13497"
FT /id="PRO_0000442656"
FT CHAIN 301..546
FT /note="Zinc metalloproteinase nas-9"
FT /id="PRO_0000028914"
FT DOMAIN 308..507
FT /note="Peptidase M12A"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01211"
FT DOMAIN 510..546
FT /note="ShKT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT ACT_SITE 402
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01211"
FT BINDING 401
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01211"
FT BINDING 405
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01211"
FT BINDING 411
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01211"
FT CARBOHYD 248
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 347..506
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01211"
FT DISULFID 372..392
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01211"
FT DISULFID 510..546
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 517..539
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 526..543
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT VAR_SEQ 1..74
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_012352"
FT VAR_SEQ 395..423
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_012353"
SQ SEQUENCE 546 AA; 60977 MW; EAB496C2E69AA476 CRC64;
MIFLLFVVFP FVYAQLLPEL LAGFQNGRFR GGPDGFNRGP GGFHRGPDGF GGDPRGGVDL
GHLIGNIAAN VGQEMGLNDA DVIGDLRGIS RGPRPSSMEW GRRARHFCRR YPGHPKCQRG
QLPQFTDVPT IINTIIYNAG DLLPRVPTLN IHDPLAGLNS ELVGFIKSLQ SQFGQLSSQQ
RNEIHDSCRS FKCDQQSPQN TQAKQELLTK MLAFDQAVGG KAAPAHDKVN LRFDRTQQVK
QALLKRANLS HIIVPADNGV FDRDVLLTEH QANFLLNELG EAGRGADVGA GGGGGGRVPR
SGVFFQESAV QKWDIWKPIQ YTLDDSLEES DKKDIRDALH EISINTCILF RYNATPKGYH
LNYMKVDSTT FCGLSYVGRT DPANPIYLSF QCGDNRGVAM HETMHALGVS HQHLRLDRDK
YIKIDWSNID PQHYDTFAIS DAKLYTSYGT KYAYDSIMHY NAYLGAKDPN KPTMIPLVNP
QENTPKLGQR AKLTRGDIRL LKKMYCRPGC DDQNVHCGTW ALHGYCKMKE QMKWMNENCK
ASCDKC
