ID   NAS9_HORVU              Reviewed;         340 AA.
AC   Q9XFB7;
DT   13-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Nicotianamine synthase 9;
DE            EC=2.5.1.43;
DE   AltName: Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 9;
GN   Name=NAS9; Synonyms=NASHOR2;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 247-258.
RC   STRAIN=cv. Bonus;
RX   PubMed=10491178; DOI=10.1046/j.1432-1327.1999.00717.x;
RA   Herbik A., Koch G., Mock H.-P., Dushkov D., Czihal A., Thielmann J.,
RA   Stephan U.W., Baeumlein H.;
RT   "Isolation, characterization and cDNA cloning of nicotianamine synthase
RT   from barley. A key enzyme for iron homeostasis in plants.";
RL   Eur. J. Biochem. 265:231-239(1999).
CC   -!- FUNCTION: Synthesizes nicotianamine, a polyamine that is the first
CC       intermediate in the synthesis of the phytosiderophores of the mugineic
CC       acid type found in gramineae which serves as a sensor for the
CC       physiological iron status within the plant, and/or might be involved in
CC       the transport of iron.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 S-adenosyl-L-methionine = 3 H(+) + nicotianamine + 3 S-
CC         methyl-5'-thioadenosine; Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC   -!- SUBUNIT: Homotrimer.
CC   -!- INDUCTION: Fivefold increase after iron deficiency.
CC   -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC       {ECO:0000305}.
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DR   EMBL; AF136942; AAD32651.1; -; mRNA.
DR   AlphaFoldDB; Q9XFB7; -.
DR   SMR; Q9XFB7; -.
DR   BRENDA; 2.5.1.43; 2687.
DR   ExpressionAtlas; Q9XFB7; baseline.
DR   GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR004298; Nicotian_synth.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR32266; PTHR32266; 2.
DR   Pfam; PF03059; NAS; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51142; NAS; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..340
FT                   /note="Nicotianamine synthase 9"
FT                   /id="PRO_0000212712"
FT   CONFLICT        247
FT                   /note="G -> S (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        252
FT                   /note="V -> I (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   340 AA;  36313 MW;  050F982054B1273A CRC64;
     MGMEGCCSNK KVMEEEALVK KITGLAAAIG ELPSLSPSPE VNALFTELVT SCIPPSTVDV
     DALGPDAQEM RARLIRLCAD AEGHLEAHYS DLLAAHDNPL DHLTLFPYFN NYIKLSQLEH
     GLLARHVPGP APARVAFLGS GPLPLSSLVL AARHLPDASF DNYDISGEAN ERASRLVRAD
     ADAGARMAFR TADVADVTTE LEGYDVVFLA ALVGMAAEEK ARLVEHLGRH MAPGAALVVR
     SAHGARGFLY PVVDPEEIRR GGFEVLTVHH PEDEVINSVI IARKAAAPPP VAADRDVPVN
     MPMPAQCAVA VSRPCLGCAC ELGARAHQKM KEIAMEEMEA