NASB_HALMT
ID NASB_HALMT Reviewed; 437 AA.
AC I3R635; Q703N4;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2012, sequence version 1.
DT 25-MAY-2022, entry version 50.
DE RecName: Full=Probable nitrate transporter {ECO:0000303|PubMed:16182473};
GN Name=nasB {ECO:0000303|PubMed:16182473}; Synonyms=narK;
GN OrderedLocusNames=HFX_2003; ORFNames=C439_11793;
OS Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC
OS 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Haloferacaceae; Haloferax.
OX NCBI_TaxID=523841;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RC STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RX PubMed=16182473; DOI=10.1016/j.gene.2005.07.011;
RA Lledo B., Marhuenda-Egea F.C., Martinez-Espinosa R.M., Bonete M.J.;
RT "Identification and transcriptional analysis of nitrate assimilation genes
RT in the halophilic archaeon Haloferax mediterranei.";
RL Gene 361:80-88(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RX PubMed=22843593; DOI=10.1128/jb.00880-12;
RA Han J., Zhang F., Hou J., Liu X., Li M., Liu H., Cai L., Zhang B., Chen Y.,
RA Zhou J., Hu S., Xiang H.;
RT "Complete genome sequence of the metabolically versatile halophilic
RT archaeon Haloferax mediterranei, a poly(3-hydroxybutyrate-co-3-
RT hydroxyvalerate) producer.";
RL J. Bacteriol. 194:4463-4464(2012).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RX PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT strategies for static and dynamic osmo-response.";
RL PLoS Genet. 10:E1004784-E1004784(2014).
CC -!- FUNCTION: Is likely responsible for nitrate uptake in the nitrate
CC assimilation pathway. {ECO:0000305|PubMed:16182473}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Repressed by the presence of ammonium as nitrogen source.
CC {ECO:0000269|PubMed:16182473}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR EMBL; AJ621499; CAF19043.1; -; Genomic_DNA.
DR EMBL; CP001868; AFK19695.1; -; Genomic_DNA.
DR EMBL; AOLO01000009; EMA00017.1; -; Genomic_DNA.
DR RefSeq; WP_004059341.1; NZ_CP007551.1.
DR AlphaFoldDB; I3R635; -.
DR SMR; I3R635; -.
DR STRING; 523841.HFX_2003; -.
DR EnsemblBacteria; AFK19695; AFK19695; HFX_2003.
DR EnsemblBacteria; EMA00017; EMA00017; C439_11793.
DR GeneID; 13028163; -.
DR GeneID; 40155087; -.
DR KEGG; hme:HFX_2003; -.
DR PATRIC; fig|523841.21.peg.2384; -.
DR eggNOG; arCOG00148; Archaea.
DR HOGENOM; CLU_024204_2_0_2; -.
DR OMA; VIVCWFN; -.
DR OrthoDB; 22040at2157; -.
DR Proteomes; UP000006469; Chromosome.
DR Proteomes; UP000011603; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR PANTHER; PTHR23515; PTHR23515; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Nitrate assimilation; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..437
FT /note="Probable nitrate transporter"
FT /id="PRO_0000428916"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 359..379
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 54..58
FT /note="AIWSA -> HLVG (in Ref. 1; CAF19043)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 437 AA; 46239 MW; 645AEE03CAA813D4 CRC64;
MGLVKMTRWR TLVLATAAFN LSFLIWFSFA PFTGQIAAEF GLSLTDLGIL ASAAIWSAPP
GRILTGWLSD RFGASTVFGI VLAYVGIFSM ASAFATSYEV FFVERVVVAS AGITFVVGIQ
HVSQWFPEEE LGTAEGIYAG IGNAGAAGGA LILPRAFGNW SGPLFSTGWR AAFFYTGVVA
ILMAIVYVVF GQDAATKARA AATSESATLS TWVHTATRYG VVALALGYVM SFGLEISMNG
WLPTYFREGF GSNLVIASTF AATFSLAAGL LRPIGGYVSD RLVRDQRDIL PFFAGRYREQ
WTVLCMTFIV VSMTGLTFAG QTGNVLLTVA AGFLVGMSCA FTEGAIFAQV PAMFPNRSGA
AAGIVGGIGT FGGIGFPLVY SFAAAEGMIH TGYVIVAALM IPILALNAFI SRPHIASRAH
VDGFFDGSRF ESSQSDD
