ID   NASR_KLEOX              Reviewed;         396 AA.
AC   Q48468;
DT   29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 2.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Nitrate regulatory protein;
GN   Name=nasR;
OS   Klebsiella oxytoca.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=571;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M5a1;
RX   PubMed=8051020; DOI=10.1128/jb.176.16.5077-5085.1994;
RA   Goldman B.S., Lin J.T., Stewart V.;
RT   "Identification and structure of the nasR gene encoding a nitrate- and
RT   nitrite-responsive positive regulator of nasFEDCBA (nitrate assimilation)
RT   operon expression in Klebsiella pneumoniae M5al.";
RL   J. Bacteriol. 176:5077-5085(1994).
RN   [2]
RP   FUNCTION.
RC   STRAIN=M5a1;
RX   PubMed=9769209; DOI=10.1006/jmbi.1998.2105;
RA   Chai W., Stewart V.;
RT   "NasR, a novel RNA-binding protein, mediates nitrate-responsive
RT   transcription antitermination of the Klebsiella oxytoca M5al nasF operon
RT   leader in vitro.";
RL   J. Mol. Biol. 283:339-351(1998).
CC   -!- FUNCTION: Nitrate- and nitrite-responsive positive regulator for
CC       nasFEDCBA operon expression. NasR protein binds to the factor-
CC       independent terminator site located in the nasF operon leader RNA to
CC       effect transcription antitermination. {ECO:0000269|PubMed:9769209}.
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DR   EMBL; L27824; AAA25101.2; -; Genomic_DNA.
DR   PIR; A55859; A55859.
DR   RefSeq; WP_004133379.1; NZ_QDDU01000001.1.
DR   PDB; 4AKK; X-ray; 2.14 A; A/B=1-396.
DR   PDBsum; 4AKK; -.
DR   AlphaFoldDB; Q48468; -.
DR   SMR; Q48468; -.
DR   STRING; 571.MC52_25315; -.
DR   GeneID; 64336940; -.
DR   PATRIC; fig|571.110.peg.3487; -.
DR   eggNOG; COG3707; Bacteria.
DR   OrthoDB; 286836at2; -.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR005561; ANTAR.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR013587; Nitrate/nitrite_sensing.
DR   InterPro; IPR010910; Nitrate/nitrite_sensing_bac.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF03861; ANTAR; 1.
DR   Pfam; PF08376; NIT; 1.
DR   SMART; SM01012; ANTAR; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS50921; ANTAR; 1.
DR   PROSITE; PS50906; NIT; 1.
PE   1: Evidence at protein level;
KW   3D-structure; RNA-binding; Transcription; Transcription antitermination;
KW   Transcription regulation.
FT   CHAIN           1..396
FT                   /note="Nitrate regulatory protein"
FT                   /id="PRO_0000096738"
FT   DOMAIN          37..284
FT                   /note="NIT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00232"
FT   DOMAIN          323..384
FT                   /note="ANTAR"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00308"
FT   HELIX           10..25
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           26..28
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           29..58
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   STRAND          61..63
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           65..90
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           95..107
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           108..110
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           111..120
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           125..140
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           142..146
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           147..149
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           153..185
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           190..213
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           216..225
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           230..240
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           250..285
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           304..312
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           314..331
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           333..353
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           358..372
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           376..385
FT                   /evidence="ECO:0007829|PDB:4AKK"
FT   HELIX           387..389
FT                   /evidence="ECO:0007829|PDB:4AKK"
SQ   SEQUENCE   396 AA;  44507 MW;  78FA2A878054884E CRC64;
     MNNMAGNTPE VVDWFARARR LQKQQLHQLA QQGTLAGQIS ALVHMLQCER GASNIWLCSG
     GRLYAAECRA GAALVDEQLT RFYAALEPAR DAASSALCWR IACAVWYLPQ LAALRKRVRD
     REIAAEEATG QFSRIIRHLL NIVPQLNDSI DDPQIAGRMV ALYSFMQGKE LAGQERALGA
     LGFARGQFSD ELRQQLVDRI DGQQPCFDSF QALAQPPQTA LFAEQCQASL EIEQLRRVAC
     TRQPPADEGE TALRWFCAQT QRLEQLRGVE ELLIVDLLNA ADALLEGEEP EAQLPPADWQ
     EDSIALRLDK QLLPLVRQQA HELQQLSGQL ASLKDALEER KLIEKAKSVL MTYQGMQEEQ
     AWQALRKMAM DKNQRMVEIA RALLTVKALW RVTPKE