ID   NAS_SOLLC               Reviewed;         317 AA.
AC   Q9XGI7;
DT   13-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Nicotianamine synthase;
DE            EC=2.5.1.43;
DE   AltName: Full=Chloronerva;
DE   AltName: Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase;
GN   Name=CHLN; Synonyms=CLN;
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND VARIANT CHLORONERVA.
RC   STRAIN=cv. Bonner Beste, and cv. Moneymaker;
RX   PubMed=10359845; DOI=10.1073/pnas.96.12.7098;
RA   Ling H.-Q., Koch G., Baeumlein H., Ganal M.W.;
RT   "Map-based cloning of chloronerva, a gene involved in iron uptake of higher
RT   plants encoding nicotianamine synthase.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:7098-7103(1999).
CC   -!- FUNCTION: Synthesizes nicotianamine, a polyamine that serves as a
CC       sensor for the physiological iron status within the plant, and/or might
CC       be involved in the transport of iron. {ECO:0000269|PubMed:10359845}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 S-adenosyl-L-methionine = 3 H(+) + nicotianamine + 3 S-
CC         methyl-5'-thioadenosine; Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC   -!- SUBUNIT: Homomultimer.
CC   -!- TISSUE SPECIFICITY: Leaves and roots.
CC   -!- INDUCTION: Expression independent of the iron status.
CC   -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC       {ECO:0000305}.
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DR   EMBL; AJ242045; CAB42052.1; -; Genomic_DNA.
DR   RefSeq; NP_001296307.1; NM_001309378.1.
DR   AlphaFoldDB; Q9XGI7; -.
DR   SMR; Q9XGI7; -.
DR   STRING; 4081.Solyc01g100490.2.1; -.
DR   PaxDb; Q9XGI7; -.
DR   PRIDE; Q9XGI7; -.
DR   EnsemblPlants; Solyc01g100490.3.1; Solyc01g100490.3.1.1; Solyc01g100490.3.
DR   GeneID; 101248619; -.
DR   Gramene; Solyc01g100490.3.1; Solyc01g100490.3.1.1; Solyc01g100490.3.
DR   KEGG; sly:101248619; -.
DR   eggNOG; ENOG502QTU6; Eukaryota.
DR   HOGENOM; CLU_031919_1_1_1; -.
DR   InParanoid; Q9XGI7; -.
DR   OMA; SERMFFH; -.
DR   OrthoDB; 1009301at2759; -.
DR   PhylomeDB; Q9XGI7; -.
DR   BioCyc; MetaCyc:MON-14045; -.
DR   BRENDA; 2.5.1.43; 3101.
DR   Proteomes; UP000004994; Chromosome 1.
DR   GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR004298; Nicotian_synth.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR32266; PTHR32266; 1.
DR   Pfam; PF03059; NAS; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51142; NAS; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..317
FT                   /note="Nicotianamine synthase"
FT                   /id="PRO_0000212713"
FT   VARIANT         238
FT                   /note="F -> S (in chloronerva; has no activity)"
SQ   SEQUENCE   317 AA;  35303 MW;  308B73A84AB30646 CRC64;
     MVCPNSNPVV EKVCELYEQI SRLENLSPSK DVNVLFTDLV HTCMPPNPID VSKLCQKIQE
     IRSHLIKLCG QAEGLLESHF SKILSSYENP LQHLHIFPYF DNYIKLSLLE YNILTKNTTN
     IPKKIAFIGS GPLPLTSLVL ATKHLKTTCF HNYDIDVDAN FMASALVAAD PDMSSRMTFH
     TADVMDVTCA LKDYDVVFLA ALVGMDKEDK VKVVDHLAKY MSPGATLMLR SAHGARAFLY
     PVLDPRDLRG FEVLSVYHPT DEVINSVIIA RKLPVPSVPL LDGLGAYVLP SKCACAEIHA
     FNPLNKMNLV EEFALEE