NAT12_ARATH
ID NAT12_ARATH Reviewed; 709 AA.
AC Q3E7D0; Q8LPL9; Q9ZUY2;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Nucleobase-ascorbate transporter 12;
DE Short=AtNAT12;
GN Name=NAT12; OrderedLocusNames=At2g27810; ORFNames=F15K20.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, SUBCELLULAR LOCATION,
RP AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=16982705; DOI=10.1093/pcp/pcl011;
RA Maurino V.G., Grube E., Zielinski J., Schild A., Fischer K., Flugge U.-I.;
RT "Identification and expression analysis of twelve members of the
RT nucleobase-ascorbate transporter (NAT) gene family in Arabidopsis
RT thaliana.";
RL Plant Cell Physiol. 47:1381-1393(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. La-0;
RX PubMed=14506206; DOI=10.1074/mcp.t300006-mcp200;
RA Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
RT "Large-scale analysis of in vivo phosphorylated membrane proteins by
RT immobilized metal ion affinity chromatography and mass spectrometry.";
RL Mol. Cell. Proteomics 2:1234-1243(2003).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=15308754; DOI=10.1105/tpc.104.023150;
RA Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
RT "Phosphoproteomics of the Arabidopsis plasma membrane and a new
RT phosphorylation site database.";
RL Plant Cell 16:2394-2405(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:16982705};
CC Multi-pass membrane protein {ECO:0000269|PubMed:16982705}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3E7D0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3E7D0-2; Sequence=VSP_022177;
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:16982705}.
CC -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC 2.A.40) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC73019.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY444865; AAR18374.1; -; mRNA.
DR EMBL; AC005824; AAC73019.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC08047.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08048.1; -; Genomic_DNA.
DR EMBL; AY099545; AAM20397.1; -; mRNA.
DR EMBL; BT002121; AAN72132.1; -; mRNA.
DR PIR; C84677; C84677.
DR RefSeq; NP_850108.1; NM_179777.2. [Q3E7D0-1]
DR RefSeq; NP_973550.2; NM_201821.2. [Q3E7D0-2]
DR AlphaFoldDB; Q3E7D0; -.
DR SMR; Q3E7D0; -.
DR STRING; 3702.AT2G27810.1; -.
DR TCDB; 2.A.40.6.6; the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.
DR iPTMnet; Q3E7D0; -.
DR PaxDb; Q3E7D0; -.
DR PRIDE; Q3E7D0; -.
DR ProteomicsDB; 251247; -. [Q3E7D0-1]
DR EnsemblPlants; AT2G27810.1; AT2G27810.1; AT2G27810. [Q3E7D0-1]
DR EnsemblPlants; AT2G27810.2; AT2G27810.2; AT2G27810. [Q3E7D0-2]
DR GeneID; 817328; -.
DR Gramene; AT2G27810.1; AT2G27810.1; AT2G27810. [Q3E7D0-1]
DR Gramene; AT2G27810.2; AT2G27810.2; AT2G27810. [Q3E7D0-2]
DR KEGG; ath:AT2G27810; -.
DR Araport; AT2G27810; -.
DR TAIR; locus:2042036; AT2G27810.
DR eggNOG; KOG1292; Eukaryota.
DR InParanoid; Q3E7D0; -.
DR OMA; HRFKHIM; -.
DR PhylomeDB; Q3E7D0; -.
DR PRO; PR:Q3E7D0; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q3E7D0; baseline and differential.
DR Genevisible; Q3E7D0; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015207; F:adenine transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015208; F:guanine transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015294; F:solute:cation symporter activity; IDA:TAIR.
DR GO; GO:0015210; F:uracil transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0098702; P:adenine import across plasma membrane; IDA:TAIR.
DR GO; GO:0098710; P:guanine import across plasma membrane; IDA:TAIR.
DR GO; GO:0035344; P:hypoxanthine transport; IDA:TAIR.
DR GO; GO:0098721; P:uracil import across plasma membrane; IDA:TAIR.
DR InterPro; IPR029946; NAT12.
DR InterPro; IPR006043; NCS2.
DR PANTHER; PTHR11119:SF28; PTHR11119:SF28; 1.
DR Pfam; PF00860; Xan_ur_permease; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..709
FT /note="Nucleobase-ascorbate transporter 12"
FT /id="PRO_0000270169"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 438..458
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 530..550
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 551..571
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 585..605
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 639..659
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..23
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 39..69
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 40
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:14506206"
FT VAR_SEQ 590..709
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_022177"
SQ SEQUENCE 709 AA; 76674 MW; BD6CE057C49EF3FB CRC64;
MSSSDPKPGP KPGPWPPTPE SAAMPPSSWA KKTGFRPKFS GETTATDSSS GQLSLPVRAK
QQETQPDLEA GQTRLRPPPP VSAAVTNGET DKDKKEKPPP PPPGSVAVPV KDQPVKRRRD
SDGVVGRSNG PDGANGSGDP VRRPGRIEET VEVLPQSMDD DLVARNLHMK YGLRDTPGLV
PIGFYGLQHY LSMLGSLILV PLVIVPAMGG SHEEVANVVS TVLFVSGITT LLHTSFGSRL
PLIQGPSFVF LAPALAIINS PEFQGLNGNN NFKHIMRELQ GAIIIGSAFQ AVLGYSGLMS
LILRLVNPVV VAPTVAAVGL SFYSYGFPLV GKCLEIGVVQ ILLVIIFALY LRKISVLSHR
IFLIYAVPLS LAITWAAAFL LTETGAYTYK GCDPNVPVSN VVSTHCRKYM TRMKYCRVDT
SHALSSAPWF RFPYPLQWGV PLFNWKMAFV MCVVSVIASV DSVGSYHASS LLVASRPPTR
GVVSRAIGLE GFTSVLAGLW GTGTGSTTLT ENVHTIAVTK MGSRRVVELG ACVLVIFSLV
GKVGGFLASI PQVMVASLLC FMWAMFTALG LSNLRYSEAG SSRNIIIVGL SLFFSLSVPA
YFQQYGISPN SNLSVPSYYQ PYIVSSHGPF KSQYKGMNYV MNTLLSMSMV IAFIMAVILD
NTVPGSKQER GVYVWSDSET ATREPALAKD YELPFRVGRF FRWVKWVGI
