NAT16_HUMAN
ID NAT16_HUMAN Reviewed; 369 AA.
AC Q8N8M0; B3KRS2; Q8NDR1;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-NOV-2009, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Probable N-acetyltransferase 16;
DE EC=2.3.1.-;
GN Name=NAT16; Synonyms=C7orf52;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT
RP SER-63.
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT SER-63.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 165-369 (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [5]
RP FUNCTION.
RX PubMed=24121108; DOI=10.1016/j.bbagen.2013.10.004;
RA Yamada S., Arikawa S.;
RT "An ectotherm homologue of human predicted gene NAT16 encodes histidine N-
RT acetyltransferase responsible for Nalpha-acetylhistidine synthesis.";
RL Biochim. Biophys. Acta 1840:434-442(2014).
CC -!- FUNCTION: Probable N-acetyltransferase. Shows only trace activity
CC toward L-His and no N-acetyltransferase activity toward other amino
CC acids. The physiological substrate of this enzyme is unknown.
CC {ECO:0000269|PubMed:24121108}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8N8M0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N8M0-2; Sequence=VSP_055771, VSP_055772;
CC -!- MISCELLANEOUS: NAT16 is the ortholog of the ectothermic vertebrates
CC enzyme HISAT (AC I3J7Q8) responsible for the synthesis of N-acetyl-
CC histidine (NAH). NAT16 protein, unlike fish HISAT, has only trace
CC enzyme activity for NAH synthesis. {ECO:0000305|PubMed:24121108}.
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DR EMBL; AK092119; BAG52484.1; -; mRNA.
DR EMBL; AK096556; BAC04815.1; -; mRNA.
DR EMBL; AC004876; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC104841; AAI04842.1; -; mRNA.
DR EMBL; AL831911; CAD38574.3; -; mRNA.
DR CCDS; CCDS5713.1; -. [Q8N8M0-1]
DR RefSeq; NP_940973.2; NM_198571.2. [Q8N8M0-1]
DR RefSeq; XP_005250382.1; XM_005250325.1.
DR RefSeq; XP_011514470.1; XM_011516168.1.
DR AlphaFoldDB; Q8N8M0; -.
DR SMR; Q8N8M0; -.
DR BioGRID; 131987; 74.
DR IntAct; Q8N8M0; 6.
DR STRING; 9606.ENSP00000300303; -.
DR iPTMnet; Q8N8M0; -.
DR PhosphoSitePlus; Q8N8M0; -.
DR BioMuta; NAT16; -.
DR DMDM; 269849548; -.
DR MassIVE; Q8N8M0; -.
DR PaxDb; Q8N8M0; -.
DR PeptideAtlas; Q8N8M0; -.
DR PRIDE; Q8N8M0; -.
DR ProteomicsDB; 3613; -.
DR ProteomicsDB; 72436; -. [Q8N8M0-1]
DR Antibodypedia; 16722; 17 antibodies from 13 providers.
DR DNASU; 375607; -.
DR Ensembl; ENST00000300303.7; ENSP00000300303.2; ENSG00000167011.9. [Q8N8M0-1]
DR Ensembl; ENST00000443096.1; ENSP00000394435.1; ENSG00000167011.9. [Q8N8M0-2]
DR Ensembl; ENST00000455377.5; ENSP00000395125.1; ENSG00000167011.9. [Q8N8M0-1]
DR GeneID; 375607; -.
DR KEGG; hsa:375607; -.
DR MANE-Select; ENST00000300303.7; ENSP00000300303.2; NM_198571.3; NP_940973.2.
DR UCSC; uc003uxy.2; human. [Q8N8M0-1]
DR CTD; 375607; -.
DR GeneCards; NAT16; -.
DR HGNC; HGNC:22030; NAT16.
DR HPA; ENSG00000167011; Group enriched (brain, pancreas, pituitary gland, retina, thyroid gland).
DR MIM; 615783; gene.
DR neXtProt; NX_Q8N8M0; -.
DR OpenTargets; ENSG00000167011; -.
DR PharmGKB; PA162380580; -.
DR VEuPathDB; HostDB:ENSG00000167011; -.
DR eggNOG; ENOG502QW73; Eukaryota.
DR GeneTree; ENSGT00390000016398; -.
DR HOGENOM; CLU_074598_0_0_1; -.
DR InParanoid; Q8N8M0; -.
DR OMA; FAVICET; -.
DR OrthoDB; 1293735at2759; -.
DR PhylomeDB; Q8N8M0; -.
DR TreeFam; TF331490; -.
DR BRENDA; 2.3.1.33; 2681.
DR PathwayCommons; Q8N8M0; -.
DR SignaLink; Q8N8M0; -.
DR BioGRID-ORCS; 375607; 10 hits in 1062 CRISPR screens.
DR GenomeRNAi; 375607; -.
DR Pharos; Q8N8M0; Tdark.
DR PRO; PR:Q8N8M0; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q8N8M0; protein.
DR Bgee; ENSG00000167011; Expressed in cortical plate and 49 other tissues.
DR ExpressionAtlas; Q8N8M0; baseline and differential.
DR Genevisible; Q8N8M0; HS.
DR GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IBA:GO_Central.
DR GO; GO:0008080; F:N-acetyltransferase activity; IEA:InterPro.
DR InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR InterPro; IPR000182; GNAT_dom.
DR SUPFAM; SSF55729; SSF55729; 1.
DR PROSITE; PS51186; GNAT; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Alternative splicing; Reference proteome; Transferase.
FT CHAIN 1..369
FT /note="Probable N-acetyltransferase 16"
FT /id="PRO_0000309189"
FT DOMAIN 53..188
FT /note="N-acetyltransferase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00532"
FT REGION 1..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..37
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 105..112
FT /note="IALESVNV -> VSPGARPR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055771"
FT VAR_SEQ 113..369
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055772"
FT VARIANT 63
FT /note="F -> S (in dbSNP:rs34985488)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_036903"
SQ SEQUENCE 369 AA; 40521 MW; DC3AD7F15AAF807C CRC64;
MKLEASCGTA TSEVPKPEKK TARDAEPSSE TRPQEVEAEP RSGSGPEAEA EPLDFVVATE
REFEEVLAIS GGIYGGLDYL PSRYHSWLRD PDRTVVLAKR NGGVIALESV NVIDAGETVL
VEGLRVAPWE RGKGVAGLLQ RFCSQLVKRQ HPGVKVARLT RDDQLGPREL KKYRLITKQG
ILLVRFNASA LLAGLGARLA ALRTSGTFSP LPTEAVSEAG GDVARLLLSP SVQRDVLPGG
TIIQDWQPYR PSESNLRLLA AKGLEWRVDS RARPRVLTLC TRPFPIPHGG DGTWRYLNID
AFGSDGAQVQ SQLLWHLQRQ APRLVGLNVM CQLFLEPQLW SQLADFCQVG LGLELVKGYT
EQYLLEADI
