ID   NAT2_ARATH              Reviewed;         524 AA.
AC   Q94C70; O80768;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Nucleobase-ascorbate transporter 2;
DE            Short=AtNAT2;
GN   Name=NAT2; OrderedLocusNames=At2g34190; ORFNames=F13P17.3;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   GENE FAMILY, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX   PubMed=16982705; DOI=10.1093/pcp/pcl011;
RA   Maurino V.G., Grube E., Zielinski J., Schild A., Fischer K., Flugge U.-I.;
RT   "Identification and expression analysis of twelve members of the
RT   nucleobase-ascorbate transporter (NAT) gene family in Arabidopsis
RT   thaliana.";
RL   Plant Cell Physiol. 47:1381-1393(2006).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in cotyledons 10 days after imbibition
CC       (DAI). Expressed in the minor and major veins of cotyledons and leaves,
CC       in the shoot apex and pedicels. Expressed in the root meristems, root
CC       tips and lateral root primordia. {ECO:0000269|PubMed:16982705}.
CC   -!- DEVELOPMENTAL STAGE: Highly expressed in gynoecium development,
CC       disappearing with maturation. {ECO:0000269|PubMed:16982705}.
CC   -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC       2.A.40) family. {ECO:0000305}.
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DR   EMBL; AC004481; AAC27395.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC08934.1; -; Genomic_DNA.
DR   EMBL; AY035127; AAK59632.1; -; mRNA.
DR   EMBL; AY142641; AAN13099.1; -; mRNA.
DR   PIR; T02307; T02307.
DR   RefSeq; NP_180966.1; NM_128970.2.
DR   AlphaFoldDB; Q94C70; -.
DR   SMR; Q94C70; -.
DR   BioGRID; 3327; 1.
DR   IntAct; Q94C70; 1.
DR   STRING; 3702.AT2G34190.1; -.
DR   PaxDb; Q94C70; -.
DR   PRIDE; Q94C70; -.
DR   ProteomicsDB; 251047; -.
DR   EnsemblPlants; AT2G34190.1; AT2G34190.1; AT2G34190.
DR   GeneID; 817980; -.
DR   Gramene; AT2G34190.1; AT2G34190.1; AT2G34190.
DR   KEGG; ath:AT2G34190; -.
DR   Araport; AT2G34190; -.
DR   TAIR; locus:2040874; AT2G34190.
DR   eggNOG; KOG1292; Eukaryota.
DR   HOGENOM; CLU_017959_5_3_1; -.
DR   InParanoid; Q94C70; -.
DR   OMA; INHGIGM; -.
DR   OrthoDB; 387031at2759; -.
DR   PhylomeDB; Q94C70; -.
DR   PRO; PR:Q94C70; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q94C70; baseline and differential.
DR   Genevisible; Q94C70; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0071702; P:organic substance transport; IEA:UniProt.
DR   InterPro; IPR035906; MetI-like_sf.
DR   InterPro; IPR029948; NAT1/2.
DR   InterPro; IPR006043; NCS2.
DR   PANTHER; PTHR11119:SF106; PTHR11119:SF106; 1.
DR   Pfam; PF00860; Xan_ur_permease; 1.
DR   SUPFAM; SSF161098; SSF161098; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..524
FT                   /note="Nucleobase-ascorbate transporter 2"
FT                   /id="PRO_0000270159"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        133..153
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        155..175
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        179..199
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        217..237
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        282..302
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        359..379
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        380..400
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        419..439
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        457..477
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        193
FT                   /note="L -> F (in Ref. 3; AAK59632)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   524 AA;  57614 MW;  14C1C0E57078AEBD CRC64;
     MDLVKPEEIS HPPMDQLQGL EYCIDSNPPW GEAIALGFEH YILALGTAVM IPSILVPMMG
     GDDGDKVRVV QTLLFLQGVN TLLQTLFGTR LPTVIGGSYA FMVPIISIIH DSSLTRIEDP
     QLRFLSTMRA VQGAIIVASS VQIILGFSQM WAICSRFFSP IGMVPVIALT GFGLFNRGFP
     VVGNCVEIGL PMLILFVIFS QYLKNFQFRQ FPVVERFALI IALIIVWAYA HVLTASGAYK
     HRPHQTQLNC RTDMSNLISS APWIKIPYPL QWGAPSFDAG HAFAMMAAVL VSLIESTGAF
     KAAARLASAT PPPPHVLSRG IGWQGIGILL NGLFGTLSGS SVSVENIGLL GSTRVGSRRV
     IQISAGFMIF FSMLGKFGAL FASIPFTIFA AVYCVLFGLV ASVGLSFLQF TNMNSLRNLF
     IVGVSLFLGL SIPEYFRDFS MKALHGPAHT NAGWFNDFLN TIFLSSPMVA LMVAVFLDNT
     LDYKETARDR GLPWWAKFRT FKGDSRNEEF YTLPFNLNRF FPPS