NAT2_YEAST
ID NAT2_YEAST Reviewed; 288 AA.
AC P37293; D6VUS8;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Putative N-terminal acetyltransferase 2;
DE EC=2.3.1.-;
DE AltName: Full=Amino-terminal, alpha-amino, acetyltransferase 2;
GN Name=NAT2; OrderedLocusNames=YGR147C; ORFNames=G6630;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8175741; DOI=10.1016/s0021-9258(17)36810-2;
RA Sherman F., Kulkarni M.S.;
RT "NAT2, an essential gene encoding methionine N alpha-acetyltransferase in
RT the yeast Saccharomyces cerevisiae.";
RL J. Biol. Chem. 269:13141-13147(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8585325; DOI=10.1002/yea.320111410;
RA Skala J., Nawrocki A., Goffeau A.;
RT "The sequence of a 27 kb segment on the right arm of chromosome VII from
RT Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2,
RT NSR1 genes and ten new open reading frames.";
RL Yeast 11:1421-1427(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Maybe involved in N-terminal acetylation of proteins. N-
CC acetylation plays a role in normal eukaryotic translation and
CC processing, protect against proteolytic degradation and protein
CC turnover.
CC -!- SUBUNIT: Heterooligomeric. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 1240 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; L25608; AAA34812.1; -; Genomic_DNA.
DR EMBL; X85807; CAA59805.1; -; Genomic_DNA.
DR EMBL; Z72932; CAA97161.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08239.1; -; Genomic_DNA.
DR PIR; S60438; S60438.
DR RefSeq; NP_011663.3; NM_001181276.3.
DR AlphaFoldDB; P37293; -.
DR BioGRID; 33395; 35.
DR STRING; 4932.YGR147C; -.
DR MaxQB; P37293; -.
DR PaxDb; P37293; -.
DR PRIDE; P37293; -.
DR EnsemblFungi; YGR147C_mRNA; YGR147C; YGR147C.
DR GeneID; 853050; -.
DR KEGG; sce:YGR147C; -.
DR SGD; S000003379; NAT2.
DR VEuPathDB; FungiDB:YGR147C; -.
DR eggNOG; KOG4526; Eukaryota.
DR GeneTree; ENSGT00940000156134; -.
DR HOGENOM; CLU_059211_2_0_1; -.
DR InParanoid; P37293; -.
DR OMA; KWFNGLM; -.
DR BioCyc; YEAST:YGR147C-MON; -.
DR PRO; PR:P37293; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P37293; protein.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0017196; P:N-terminal peptidyl-methionine acetylation; IMP:SGD.
DR InterPro; IPR045866; FAM210A/B-like.
DR InterPro; IPR009688; FAM210A/B-like_dom.
DR PANTHER; PTHR21377; PTHR21377; 1.
DR Pfam; PF06916; DUF1279; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Cytoplasm; Reference proteome; Transferase.
FT CHAIN 1..288
FT /note="Putative N-terminal acetyltransferase 2"
FT /id="PRO_0000096740"
FT REGION 68..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 68..88
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 61
FT /note="Q -> K (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 63
FT /note="R -> S (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 218
FT /note="V -> L (in Ref. 1; AAA34812)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 288 AA; 32804 MW; C259310FD2D2B080 CRC64;
MMVPRISASP VFKRIFLRWG FVTLPIQKTV SHTLRRDFSA PCRSMVKCLL LRPGISVHSA
QDRKFYSTEE KSSQFDENKS KSNNGKKNEP HGIKGLMAKY GYSALIVYIL LTCVDLPLCF
LGVHSLGEEK IKIYLNRGKQ LIGMGEPDES KVIQDVRRKQ AHREAVQAEN ADKVEDASRK
TFNERWQEMK DSTLLAELLI AYGIHKSLII VRVPLTAVLT PSFVKLLQRF GIDLMKKQKK
VFQTMASGAK IRYKGNNPSD FIKNEGTALD ITKRKPRTKG QKWFDGLM
