NAT3_ARATH
ID NAT3_ARATH Reviewed; 551 AA.
AC Q8GZD4; O48718;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Nucleobase-ascorbate transporter 3;
DE Short=AtNAT3;
GN Name=NAT3; OrderedLocusNames=At2g26510; ORFNames=T9J22.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Park S., Thornburg R.W.;
RT "Sequence analysis of a putative membrane transporter cDNA of Arabidopsis
RT thaliana.";
RL Hangug Nonghwahag Hoeji 45:153-159(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=16982705; DOI=10.1093/pcp/pcl011;
RA Maurino V.G., Grube E., Zielinski J., Schild A., Fischer K., Flugge U.-I.;
RT "Identification and expression analysis of twelve members of the
RT nucleobase-ascorbate transporter (NAT) gene family in Arabidopsis
RT thaliana.";
RL Plant Cell Physiol. 47:1381-1393(2006).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8GZD4-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in the apical meristem 4 days after
CC imbibition (DAI). Expressed in the major veins of rosette leaves and
CC pedicels. Expressed in the root central cylinder, root meristems, root
CC tips and lateral root primordia. {ECO:0000269|PubMed:16982705}.
CC -!- DEVELOPMENTAL STAGE: Expressed in gynoecium development, disappearing
CC after pollination. {ECO:0000269|PubMed:16982705}.
CC -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC 2.A.40) family. {ECO:0000305}.
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DR EMBL; AF515778; AAO13361.1; -; mRNA.
DR EMBL; AC002505; AAC14499.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07848.1; -; Genomic_DNA.
DR EMBL; AF370518; AAK43895.1; -; mRNA.
DR EMBL; BT008902; AAP68341.1; -; mRNA.
DR PIR; T00984; T00984.
DR RefSeq; NP_180219.1; NM_128208.5. [Q8GZD4-1]
DR AlphaFoldDB; Q8GZD4; -.
DR SMR; Q8GZD4; -.
DR BioGRID; 2544; 2.
DR STRING; 3702.AT2G26510.1; -.
DR iPTMnet; Q8GZD4; -.
DR PaxDb; Q8GZD4; -.
DR PRIDE; Q8GZD4; -.
DR ProteomicsDB; 251048; -. [Q8GZD4-1]
DR EnsemblPlants; AT2G26510.1; AT2G26510.1; AT2G26510. [Q8GZD4-1]
DR GeneID; 817192; -.
DR Gramene; AT2G26510.1; AT2G26510.1; AT2G26510. [Q8GZD4-1]
DR KEGG; ath:AT2G26510; -.
DR Araport; AT2G26510; -.
DR TAIR; locus:2066190; AT2G26510.
DR eggNOG; KOG1292; Eukaryota.
DR HOGENOM; CLU_017959_5_3_1; -.
DR InParanoid; Q8GZD4; -.
DR OMA; FGLCPKF; -.
DR OrthoDB; 387031at2759; -.
DR PhylomeDB; Q8GZD4; -.
DR PRO; PR:Q8GZD4; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8GZD4; baseline and differential.
DR Genevisible; Q8GZD4; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0015207; F:adenine transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015208; F:guanine transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015294; F:solute:cation symporter activity; IDA:TAIR.
DR GO; GO:0015210; F:uracil transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0098702; P:adenine import across plasma membrane; IDA:TAIR.
DR GO; GO:0098710; P:guanine import across plasma membrane; IDA:TAIR.
DR GO; GO:0035344; P:hypoxanthine transport; IDA:TAIR.
DR GO; GO:0098721; P:uracil import across plasma membrane; IDA:TAIR.
DR InterPro; IPR029943; NAT3.
DR InterPro; IPR006043; NCS2.
DR PANTHER; PTHR11119:SF29; PTHR11119:SF29; 1.
DR Pfam; PF00860; Xan_ur_permease; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..551
FT /note="Nucleobase-ascorbate transporter 3"
FT /id="PRO_0000270160"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 306..326
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 390..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 412..432
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 481..501
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 166..168
FT /note="NII -> TSL (in Ref. 1; AAO13361)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 551 AA; 60170 MW; 52A67E990C3AA2CC CRC64;
MVETGHHHQH PPAPAAAGHP PVPSMAMARN MGTTWPPAEQ LHHLQYCIHS NPSWHETVVL
AFQHYIVMLG TTVLIANTLV SPMGGDPGDK ARVIQTILFM SGINTLLQTL IGTRLPTVMG
VSFAYVLPVL SIIRDYNNGQ FDSEKQRFRH TMRTVQGSLI ISSFVNIIIG YGQAWGNLIR
IFSPIIVVPV VSVVSLGLFL RGFPLLANCV EIGLPMLILL IITQQYLKHA FSRISMILER
YALLVCLAII WAFAAILTVS GAYNNVSTAT KQSCRTDRAF LMSSAPWIRI PYPFQWGTPI
FKASHVFGMF GAAIVASAES TGVFFAASRL AGATAPPAHV VSRSIGLQGI GVLLEGIFGS
ITGNTASVEN VGLLGLTRIG SRRVVQVSTF FMIFFSIFGK FGAFFASIPL PIFAGVYCIL
LGIVVAVGIS FIQFTDTNSM RNMYVIGVSL FLSLSIAQYF LANTSRAGYG PVRTAGGWFN
DILNTIFASA PLVATILATI LDNTLEARHA SDDARGIPWW KPFQHRNGDG RNDEFYSMPL
RINELMPTRF L
