NAT4_ARATH
ID NAT4_ARATH Reviewed; 526 AA.
AC P93039; Q93ZL2; Q9LPM0;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Nucleobase-ascorbate transporter 4;
DE Short=AtNAT4;
DE Short=AtPER;
GN Name=NAT4; OrderedLocusNames=At1g49960; ORFNames=F2J10.14, F2J10.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-263.
RC STRAIN=cv. Columbia;
RA Sprenger M., Weisshaar B.;
RT "Identification of an Arabidopsis gene encoding a protein with high
RT similarity to a Zea mays leaf permease.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND TISSUE SPECIFICITY.
RX PubMed=16982705; DOI=10.1093/pcp/pcl011;
RA Maurino V.G., Grube E., Zielinski J., Schild A., Fischer K., Flugge U.-I.;
RT "Identification and expression analysis of twelve members of the
RT nucleobase-ascorbate transporter (NAT) gene family in Arabidopsis
RT thaliana.";
RL Plant Cell Physiol. 47:1381-1393(2006).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Highly expressed in the root central cylinder.
CC Expressed in the filaments and stigmatic papillae of pollinated flowers
CC and developing siliques. {ECO:0000269|PubMed:16982705}.
CC -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC 2.A.40) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB41234.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF76447.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC015445; AAF76447.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE32500.1; -; Genomic_DNA.
DR EMBL; AY056808; AAL10499.1; -; mRNA.
DR EMBL; AY099842; AAM20693.1; -; mRNA.
DR EMBL; BT000337; AAN15656.1; -; mRNA.
DR EMBL; U83501; AAB41234.1; ALT_SEQ; Genomic_DNA.
DR PIR; A96536; A96536.
DR RefSeq; NP_175418.1; NM_103883.2.
DR AlphaFoldDB; P93039; -.
DR SMR; P93039; -.
DR STRING; 3702.AT1G49960.1; -.
DR PaxDb; P93039; -.
DR PRIDE; P93039; -.
DR ProteomicsDB; 251094; -.
DR EnsemblPlants; AT1G49960.1; AT1G49960.1; AT1G49960.
DR GeneID; 841419; -.
DR Gramene; AT1G49960.1; AT1G49960.1; AT1G49960.
DR KEGG; ath:AT1G49960; -.
DR Araport; AT1G49960; -.
DR TAIR; locus:2031085; AT1G49960.
DR eggNOG; KOG1292; Eukaryota.
DR HOGENOM; CLU_017959_5_3_1; -.
DR InParanoid; P93039; -.
DR OMA; MCSAFAQ; -.
DR OrthoDB; 387031at2759; -.
DR PhylomeDB; P93039; -.
DR PRO; PR:P93039; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P93039; baseline and differential.
DR Genevisible; P93039; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0071702; P:organic substance transport; IEA:UniProt.
DR InterPro; IPR006043; NCS2.
DR Pfam; PF00860; Xan_ur_permease; 1.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..526
FT /note="Nucleobase-ascorbate transporter 4"
FT /id="PRO_0000270161"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..177
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 359..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 388..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 420..440
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 457..477
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 526 AA; 57818 MW; 670F594335E7D67C CRC64;
MATKTDDFAP FPVKDQLPGV EFCVSSSPNW PEGIVLGFQH YIVMLGTTVI IPSILVPLMG
GGDVEKAEVI NTVLFVSGIN TLLQSLFGSR LPVVMGASYA YLIPALYITF SYRFTYYLHP
HLRFEETMRA IQGALIIASI SHMIMGFFGL WRILVRFLSP LSAAPLVILT GVGLLAFAFP
QLARCIEIGL PALIILIILS QYLPHLFKCK RSICEQFAVL FTIAIVWAYA EILTAAGAYD
KRPDNTQLSC RTDRSGLISA SPWVRIPYPL QWGRPSFHGS DAFAMMAATY VAIVETTGSF
IAASRFGSAT HIPPSVLSRG IGWQGIGVLL NGLFGTATGS TALVENTGLL GLTKVGSRRV
VQISAGFMIF FSIFGKFGAV LASIPLPIFA ALYCVLFAYV ASAGLGLLQF CNLNSFRNKF
ILGFSIFIGL SVAQYFTEYL FISGRGPVHT RTSAFNVIMQ VIFSSAATVG IMAAFLLDCT
HSYGHASVRR DSGRHWWEKF RVYHTDTRTE EFYALPYNLN RFFPSF
