NAT5_ARATH
ID NAT5_ARATH Reviewed; 528 AA.
AC Q8RWE9; Q9FGR4;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Nucleobase-ascorbate transporter 5;
DE Short=AtNAT5;
GN Name=NAT5; OrderedLocusNames=At5g49990; ORFNames=K9P8.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND TISSUE SPECIFICITY.
RX PubMed=16982705; DOI=10.1093/pcp/pcl011;
RA Maurino V.G., Grube E., Zielinski J., Schild A., Fischer K., Flugge U.-I.;
RT "Identification and expression analysis of twelve members of the
RT nucleobase-ascorbate transporter (NAT) gene family in Arabidopsis
RT thaliana.";
RL Plant Cell Physiol. 47:1381-1393(2006).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Weakly expressed in the vasculature of developing
CC leaves. {ECO:0000269|PubMed:16982705}.
CC -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC 2.A.40) family. {ECO:0000305}.
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DR EMBL; AB024032; BAB08803.1; -; Genomic_DNA.
DR EMBL; AB026650; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED95881.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69095.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69096.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69097.1; -; Genomic_DNA.
DR EMBL; AY093137; AAM13136.1; -; mRNA.
DR EMBL; BT008887; AAP68326.1; -; mRNA.
DR EMBL; AK229752; BAF01588.1; -; mRNA.
DR RefSeq; NP_001330797.1; NM_001344866.1.
DR RefSeq; NP_001330798.1; NM_001344868.1.
DR RefSeq; NP_001330799.1; NM_001344867.1.
DR RefSeq; NP_199810.2; NM_124378.6.
DR AlphaFoldDB; Q8RWE9; -.
DR SMR; Q8RWE9; -.
DR BioGRID; 20309; 6.
DR IntAct; Q8RWE9; 6.
DR STRING; 3702.AT5G49990.1; -.
DR PaxDb; Q8RWE9; -.
DR PRIDE; Q8RWE9; -.
DR ProteomicsDB; 251050; -.
DR EnsemblPlants; AT5G49990.1; AT5G49990.1; AT5G49990.
DR EnsemblPlants; AT5G49990.2; AT5G49990.2; AT5G49990.
DR EnsemblPlants; AT5G49990.3; AT5G49990.3; AT5G49990.
DR EnsemblPlants; AT5G49990.4; AT5G49990.4; AT5G49990.
DR GeneID; 835063; -.
DR Gramene; AT5G49990.1; AT5G49990.1; AT5G49990.
DR Gramene; AT5G49990.2; AT5G49990.2; AT5G49990.
DR Gramene; AT5G49990.3; AT5G49990.3; AT5G49990.
DR Gramene; AT5G49990.4; AT5G49990.4; AT5G49990.
DR KEGG; ath:AT5G49990; -.
DR Araport; AT5G49990; -.
DR TAIR; locus:2158829; AT5G49990.
DR eggNOG; KOG1292; Eukaryota.
DR HOGENOM; CLU_017959_5_3_1; -.
DR InParanoid; Q8RWE9; -.
DR OMA; IHTGARW; -.
DR OrthoDB; 387031at2759; -.
DR PhylomeDB; Q8RWE9; -.
DR PRO; PR:Q8RWE9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8RWE9; baseline and differential.
DR Genevisible; Q8RWE9; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0071702; P:organic substance transport; IEA:UniProt.
DR InterPro; IPR006043; NCS2.
DR Pfam; PF00860; Xan_ur_permease; 1.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..528
FT /note="Nucleobase-ascorbate transporter 5"
FT /id="PRO_0000270162"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 367..387
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 390..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 460..479
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 528 AA; 57321 MW; C39B34A71260E9EF CRC64;
MSAPKSGGDP LPHPPKEQLP DISYCITSPP PWPEAVLLGF QHYLVMLGTT VLIPSALVPQ
MGGRNEEKAK LIQTILFVAG LNTLLQTVFG TRLPAVIGAS YTFVPVTISI MLSGRFNDVA
DPVERFKRII RATQGALIVA STLQIILGFS GLWRNVVRFL SPLSAAPLVG LVGYGLYELG
FPGVAKCIEI GLPGLIILIL ISQYMPHVIK GGKHVFARFA VIFSVAIVWL YAFFLTLGGA
YNGVGTDTQR SCRTDRAGLI SAAPWIRVPW PFQWGAPLFD AGEAFAMMMA SFVALVESTG
AFIAVSRYAS ATMPPPSVIS RGVGWQGVAI LISGLFGTGI GSSVSVENAG LLALTKIGSR
RVVQISAGFM IFFSILGKFG AVFASIPSPI IAALYCLFFA YVGAGGLSLL QFCNLNSFRT
LFILGFSIFL GLSIPQYFNE HTAIKGYGPV HTGARWFNDM VNVPFSSKAF VGGCVAYLLD
TTLHKKDGSI RKDRGKHWWD RFWTFKNDPR TEEFYALPFN LNKYFPSV
