ID   NAT6_ARATH              Reviewed;         532 AA.
AC   Q27GI3; Q3E869; Q94CA7; Q9FM07;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Nucleobase-ascorbate transporter 6;
DE            Short=AtNAT6;
GN   Name=NAT6; OrderedLocusNames=At5g62890; ORFNames=MQB2.190, MQB2.21;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Columbia;
RA   Li Q., Schultes N.P.;
RT   "Arabidopsis thaliana locus At5g62890, a nucleobase-ascorbate transporter
RT   family member, is preferentially expressed in carpel transmitting tract and
RT   tapetal cells.";
RL   Plant Sci. 163:233-240(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA   Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT   features of the regions of 1,456,315 bp covered by nineteen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:41-54(1998).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 173-532 (ISOFORM 1).
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [5]
RP   GENE FAMILY, AND TISSUE SPECIFICITY.
RX   PubMed=16982705; DOI=10.1093/pcp/pcl011;
RA   Maurino V.G., Grube E., Zielinski J., Schild A., Fischer K., Flugge U.-I.;
RT   "Identification and expression analysis of twelve members of the
RT   nucleobase-ascorbate transporter (NAT) gene family in Arabidopsis
RT   thaliana.";
RL   Plant Cell Physiol. 47:1381-1393(2006).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q27GI3-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q27GI3-2; Sequence=VSP_022175, VSP_022176;
CC   -!- TISSUE SPECIFICITY: Expressed in the apical region of cotyledons 4 days
CC       after imbibition (DAI). Expressed in the whole vasculature at 12 DAI.
CC       Expressed in the root central cylinder and lateral root primordia.
CC       Expressed in the vasculature of sepals, filaments, carpels and
CC       developing siliques. {ECO:0000269|PubMed:16982705, ECO:0000269|Ref.1}.
CC   -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC       2.A.40) family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF466198; AAM47573.1; -; mRNA.
DR   EMBL; AB009053; BAB10858.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED97668.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED97669.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED97670.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED97671.1; -; Genomic_DNA.
DR   EMBL; AY035003; AAK59508.2; -; mRNA.
DR   RefSeq; NP_001032127.2; NM_001037050.2. [Q27GI3-1]
DR   RefSeq; NP_001078782.1; NM_001085313.2. [Q27GI3-2]
DR   RefSeq; NP_201094.1; NM_125683.4. [Q27GI3-1]
DR   RefSeq; NP_851251.2; NM_180920.3. [Q27GI3-1]
DR   AlphaFoldDB; Q27GI3; -.
DR   SMR; Q27GI3; -.
DR   BioGRID; 21652; 1.
DR   IntAct; Q27GI3; 1.
DR   STRING; 3702.AT5G62890.2; -.
DR   iPTMnet; Q27GI3; -.
DR   PaxDb; Q27GI3; -.
DR   PRIDE; Q27GI3; -.
DR   ProteomicsDB; 251299; -. [Q27GI3-1]
DR   EnsemblPlants; AT5G62890.1; AT5G62890.1; AT5G62890. [Q27GI3-1]
DR   EnsemblPlants; AT5G62890.2; AT5G62890.2; AT5G62890. [Q27GI3-1]
DR   EnsemblPlants; AT5G62890.3; AT5G62890.3; AT5G62890. [Q27GI3-1]
DR   EnsemblPlants; AT5G62890.4; AT5G62890.4; AT5G62890. [Q27GI3-2]
DR   GeneID; 836409; -.
DR   Gramene; AT5G62890.1; AT5G62890.1; AT5G62890. [Q27GI3-1]
DR   Gramene; AT5G62890.2; AT5G62890.2; AT5G62890. [Q27GI3-1]
DR   Gramene; AT5G62890.3; AT5G62890.3; AT5G62890. [Q27GI3-1]
DR   Gramene; AT5G62890.4; AT5G62890.4; AT5G62890. [Q27GI3-2]
DR   KEGG; ath:AT5G62890; -.
DR   Araport; AT5G62890; -.
DR   TAIR; locus:2170783; AT5G62890.
DR   eggNOG; KOG1292; Eukaryota.
DR   HOGENOM; CLU_017959_5_3_1; -.
DR   InParanoid; Q27GI3; -.
DR   OMA; RKSAPFF; -.
DR   PhylomeDB; Q27GI3; -.
DR   PRO; PR:Q27GI3; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q27GI3; baseline and differential.
DR   Genevisible; Q27GI3; AT.
DR   GO; GO:0005783; C:endoplasmic reticulum; HDA:TAIR.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009505; C:plant-type cell wall; HDA:TAIR.
DR   GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR   GO; GO:0005773; C:vacuole; HDA:TAIR.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0071702; P:organic substance transport; IEA:UniProt.
DR   InterPro; IPR006043; NCS2.
DR   Pfam; PF00860; Xan_ur_permease; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..532
FT                   /note="Nucleobase-ascorbate transporter 6"
FT                   /id="PRO_0000270163"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        75..95
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        97..117
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        137..157
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        163..185
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        192..212
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        223..243
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        289..309
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        361..381
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        392..414
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        426..446
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        463..483
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         461..476
FT                   /note="FNDMVNVPFSSEPFVA -> VCRTKSLLFLLLFPLK (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_022175"
FT   VAR_SEQ         477..532
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_022176"
SQ   SEQUENCE   532 AA;  57580 MW;  D2299559E0A647CF CRC64;
     MAGGGAPAPK ADEPQPHPPK DQLPNISYCI TSPPPWPEAI LLGFQHYLVM LGTTVLIPTA
     LVPQMGGGYE EKAKVIQTIL FVAGINTLLQ TLFGTRLPAV VGASYTFVPT TISIILSGRF
     SDTSNPIDRF ERIMRATQGA LIVASTLQMI LGFSGLWRNV VRFLSPISAV PLVGLVGFGL
     YEFGFPGVAK CIEIGLPELL ILVFVSQYLP HVIKSGKNVF DRFAVIFAVV IVWIYAHLLT
     VGGAYNGAAP TTQTSCRTDR AGIIGAAPWI RVPWPFQWGA PSFDAGEAFA MMMASFVALV
     ESTGAFVAVS RYASATMLPP SILSRGIGWQ GVAILISGLF GTGAGSSVSV ENAGLLALTR
     VGSRRVVQIA AGFMIFFSIL GKFGAVFASI PAPIIAALYC LFFAYVGAGG LSFLQFCNLN
     SFRTKFILGF SVFLGLSIPQ YFNEYTAIKG YGPVHTGARW FNDMVNVPFS SEPFVAGSVA
     FFLDNTLHKK DSSIRKDRGK HWWDKFRSFK GDTRSEEFYS LPFNLNKYFP SV