NAT6_ARATH
ID NAT6_ARATH Reviewed; 532 AA.
AC Q27GI3; Q3E869; Q94CA7; Q9FM07;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Nucleobase-ascorbate transporter 6;
DE Short=AtNAT6;
GN Name=NAT6; OrderedLocusNames=At5g62890; ORFNames=MQB2.190, MQB2.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RA Li Q., Schultes N.P.;
RT "Arabidopsis thaliana locus At5g62890, a nucleobase-ascorbate transporter
RT family member, is preferentially expressed in carpel transmitting tract and
RT tapetal cells.";
RL Plant Sci. 163:233-240(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 173-532 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY, AND TISSUE SPECIFICITY.
RX PubMed=16982705; DOI=10.1093/pcp/pcl011;
RA Maurino V.G., Grube E., Zielinski J., Schild A., Fischer K., Flugge U.-I.;
RT "Identification and expression analysis of twelve members of the
RT nucleobase-ascorbate transporter (NAT) gene family in Arabidopsis
RT thaliana.";
RL Plant Cell Physiol. 47:1381-1393(2006).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q27GI3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q27GI3-2; Sequence=VSP_022175, VSP_022176;
CC -!- TISSUE SPECIFICITY: Expressed in the apical region of cotyledons 4 days
CC after imbibition (DAI). Expressed in the whole vasculature at 12 DAI.
CC Expressed in the root central cylinder and lateral root primordia.
CC Expressed in the vasculature of sepals, filaments, carpels and
CC developing siliques. {ECO:0000269|PubMed:16982705, ECO:0000269|Ref.1}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC 2.A.40) family. {ECO:0000305}.
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DR EMBL; AF466198; AAM47573.1; -; mRNA.
DR EMBL; AB009053; BAB10858.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97668.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97669.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97670.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97671.1; -; Genomic_DNA.
DR EMBL; AY035003; AAK59508.2; -; mRNA.
DR RefSeq; NP_001032127.2; NM_001037050.2. [Q27GI3-1]
DR RefSeq; NP_001078782.1; NM_001085313.2. [Q27GI3-2]
DR RefSeq; NP_201094.1; NM_125683.4. [Q27GI3-1]
DR RefSeq; NP_851251.2; NM_180920.3. [Q27GI3-1]
DR AlphaFoldDB; Q27GI3; -.
DR SMR; Q27GI3; -.
DR BioGRID; 21652; 1.
DR IntAct; Q27GI3; 1.
DR STRING; 3702.AT5G62890.2; -.
DR iPTMnet; Q27GI3; -.
DR PaxDb; Q27GI3; -.
DR PRIDE; Q27GI3; -.
DR ProteomicsDB; 251299; -. [Q27GI3-1]
DR EnsemblPlants; AT5G62890.1; AT5G62890.1; AT5G62890. [Q27GI3-1]
DR EnsemblPlants; AT5G62890.2; AT5G62890.2; AT5G62890. [Q27GI3-1]
DR EnsemblPlants; AT5G62890.3; AT5G62890.3; AT5G62890. [Q27GI3-1]
DR EnsemblPlants; AT5G62890.4; AT5G62890.4; AT5G62890. [Q27GI3-2]
DR GeneID; 836409; -.
DR Gramene; AT5G62890.1; AT5G62890.1; AT5G62890. [Q27GI3-1]
DR Gramene; AT5G62890.2; AT5G62890.2; AT5G62890. [Q27GI3-1]
DR Gramene; AT5G62890.3; AT5G62890.3; AT5G62890. [Q27GI3-1]
DR Gramene; AT5G62890.4; AT5G62890.4; AT5G62890. [Q27GI3-2]
DR KEGG; ath:AT5G62890; -.
DR Araport; AT5G62890; -.
DR TAIR; locus:2170783; AT5G62890.
DR eggNOG; KOG1292; Eukaryota.
DR HOGENOM; CLU_017959_5_3_1; -.
DR InParanoid; Q27GI3; -.
DR OMA; RKSAPFF; -.
DR PhylomeDB; Q27GI3; -.
DR PRO; PR:Q27GI3; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q27GI3; baseline and differential.
DR Genevisible; Q27GI3; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009505; C:plant-type cell wall; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0005773; C:vacuole; HDA:TAIR.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0071702; P:organic substance transport; IEA:UniProt.
DR InterPro; IPR006043; NCS2.
DR Pfam; PF00860; Xan_ur_permease; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..532
FT /note="Nucleobase-ascorbate transporter 6"
FT /id="PRO_0000270163"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 192..212
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..414
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 426..446
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 463..483
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 461..476
FT /note="FNDMVNVPFSSEPFVA -> VCRTKSLLFLLLFPLK (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_022175"
FT VAR_SEQ 477..532
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_022176"
SQ SEQUENCE 532 AA; 57580 MW; D2299559E0A647CF CRC64;
MAGGGAPAPK ADEPQPHPPK DQLPNISYCI TSPPPWPEAI LLGFQHYLVM LGTTVLIPTA
LVPQMGGGYE EKAKVIQTIL FVAGINTLLQ TLFGTRLPAV VGASYTFVPT TISIILSGRF
SDTSNPIDRF ERIMRATQGA LIVASTLQMI LGFSGLWRNV VRFLSPISAV PLVGLVGFGL
YEFGFPGVAK CIEIGLPELL ILVFVSQYLP HVIKSGKNVF DRFAVIFAVV IVWIYAHLLT
VGGAYNGAAP TTQTSCRTDR AGIIGAAPWI RVPWPFQWGA PSFDAGEAFA MMMASFVALV
ESTGAFVAVS RYASATMLPP SILSRGIGWQ GVAILISGLF GTGAGSSVSV ENAGLLALTR
VGSRRVVQIA AGFMIFFSIL GKFGAVFASI PAPIIAALYC LFFAYVGAGG LSFLQFCNLN
SFRTKFILGF SVFLGLSIPQ YFNEYTAIKG YGPVHTGARW FNDMVNVPFS SEPFVAGSVA
FFLDNTLHKK DSSIRKDRGK HWWDKFRSFK GDTRSEEFYS LPFNLNKYFP SV