ID   NAT8L_DANRE             Reviewed;         282 AA.
AC   A4IGD2;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=N-acetylaspartate synthetase;
DE            Short=NAA synthetase;
DE            EC=2.3.1.17 {ECO:0000250|UniProtKB:Q8N9F0};
DE   AltName: Full=N-acetyltransferase 8-like protein;
GN   Name=nat8l; ORFNames=zgc:162648;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the synthesis of N-acetylaspartate acid (NAA) from
CC       L-aspartate and acetyl-CoA. {ECO:0000250|UniProtKB:Q8N9F0}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + L-aspartate = CoA + H(+) + N-acetyl-L-aspartate;
CC         Xref=Rhea:RHEA:14165, ChEBI:CHEBI:15378, ChEBI:CHEBI:16953,
CC         ChEBI:CHEBI:29991, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.17;
CC         Evidence={ECO:0000250|UniProtKB:Q8N9F0};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:14166;
CC         Evidence={ECO:0000250|UniProtKB:Q8N9F0};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8N9F0}.
CC       Microsome membrane {ECO:0000250|UniProtKB:D3ZVU9}; Single-pass membrane
CC       protein {ECO:0000255}. Mitochondrion membrane
CC       {ECO:0000250|UniProtKB:Q8N9F0}; Single-pass membrane protein
CC       {ECO:0000255}. Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:Q3UGX3}; Single-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the camello family. {ECO:0000305}.
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DR   EMBL; BC135040; AAI35041.1; -; mRNA.
DR   RefSeq; NP_001077308.1; NM_001083839.2.
DR   AlphaFoldDB; A4IGD2; -.
DR   STRING; 7955.ENSDARP00000112150; -.
DR   PaxDb; A4IGD2; -.
DR   Ensembl; ENSDART00000121684; ENSDARP00000112150; ENSDARG00000077256.
DR   Ensembl; ENSDART00000185657; ENSDARP00000150858; ENSDARG00000115322.
DR   GeneID; 564754; -.
DR   KEGG; dre:564754; -.
DR   CTD; 339983; -.
DR   ZFIN; ZDB-GENE-030729-4; nat8l.
DR   eggNOG; KOG3139; Eukaryota.
DR   GeneTree; ENSGT00950000182932; -.
DR   HOGENOM; CLU_013985_10_0_1; -.
DR   InParanoid; A4IGD2; -.
DR   OMA; HCGPPDM; -.
DR   OrthoDB; 1341682at2759; -.
DR   PhylomeDB; A4IGD2; -.
DR   Reactome; R-DRE-8963693; Aspartate and asparagine metabolism.
DR   PRO; PR:A4IGD2; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 7.
DR   Bgee; ENSDARG00000077256; Expressed in brain and 12 other tissues.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; ISS:UniProtKB.
DR   GO; GO:0031966; C:mitochondrial membrane; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0017188; F:aspartate N-acetyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0008080; F:N-acetyltransferase activity; IBA:GO_Central.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR000182; GNAT_dom.
DR   Pfam; PF00583; Acetyltransf_1; 1.
DR   SUPFAM; SSF55729; SSF55729; 1.
DR   PROSITE; PS51186; GNAT; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Cytoplasm; Endoplasmic reticulum; Membrane; Microsome;
KW   Mitochondrion; Reference proteome; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..282
FT                   /note="N-acetylaspartate synthetase"
FT                   /id="PRO_0000305231"
FT   TRANSMEM        103..125
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          110..269
FT                   /note="N-acetyltransferase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00532"
SQ   SEQUENCE   282 AA;  32270 MW;  271B3DC5C98A1D7A CRC64;
     MHCSSPKMVC ETKIVADEHE AIAGTKKDSI IVSSSQMWTS SSASPSALES KIEKRNQVFI
     REFERSDHEE VRRIFNEGIM ERIPNSAFRG LKQQTTTQFM YAFLTVMCYV MTKSFTLTFC
     APFILMGARY YYSRKVILSY LDCALHTDMA DIEAYYMKPT GSCFWVAVLQ GQVVGIVAAQ
     SREDDNTVEL RRMSVDSHFR GKGIAKALGR RVIEFAMLNN YSAVVLGTTA VKMAAHKLYE
     SLGFRRVGET EDYTLPGMTR SPLERLFFQI RYSHYRLQLH EE