ID   NAT8L_XENTR             Reviewed;         271 AA.
AC   A4II32;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=N-acetylaspartate synthetase;
DE            Short=NAA synthetase;
DE            EC=2.3.1.17 {ECO:0000250|UniProtKB:Q8N9F0};
DE   AltName: Full=N-acetyltransferase 8-like protein;
GN   Name=nat8l;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the synthesis of N-acetylaspartate acid (NAA) from
CC       L-aspartate and acetyl-CoA. {ECO:0000250|UniProtKB:Q8N9F0}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + L-aspartate = CoA + H(+) + N-acetyl-L-aspartate;
CC         Xref=Rhea:RHEA:14165, ChEBI:CHEBI:15378, ChEBI:CHEBI:16953,
CC         ChEBI:CHEBI:29991, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.17;
CC         Evidence={ECO:0000250|UniProtKB:Q8N9F0};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:14166;
CC         Evidence={ECO:0000250|UniProtKB:Q8N9F0};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8N9F0}.
CC       Microsome membrane {ECO:0000250|UniProtKB:D3ZVU9}; Single-pass membrane
CC       protein {ECO:0000255}. Mitochondrion membrane
CC       {ECO:0000250|UniProtKB:Q8N9F0}; Single-pass membrane protein
CC       {ECO:0000255}. Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:Q3UGX3}; Single-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the camello family. {ECO:0000305}.
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DR   EMBL; BC135824; AAI35825.1; -; mRNA.
DR   RefSeq; NP_001096350.1; NM_001102880.1.
DR   AlphaFoldDB; A4II32; -.
DR   SMR; A4II32; -.
DR   STRING; 8364.ENSXETP00000052747; -.
DR   PaxDb; A4II32; -.
DR   DNASU; 100124940; -.
DR   GeneID; 100124940; -.
DR   KEGG; xtr:100124940; -.
DR   CTD; 339983; -.
DR   Xenbase; XB-GENE-5817008; nat8l.
DR   eggNOG; KOG3139; Eukaryota.
DR   InParanoid; A4II32; -.
DR   OrthoDB; 1341682at2759; -.
DR   Reactome; R-XTR-8963693; Aspartate and asparagine metabolism.
DR   Proteomes; UP000008143; Chromosome 1.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; ISS:UniProtKB.
DR   GO; GO:0031966; C:mitochondrial membrane; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0017188; F:aspartate N-acetyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0008080; F:N-acetyltransferase activity; IBA:GO_Central.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR000182; GNAT_dom.
DR   Pfam; PF00583; Acetyltransf_1; 1.
DR   SUPFAM; SSF55729; SSF55729; 1.
DR   PROSITE; PS51186; GNAT; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Cytoplasm; Endoplasmic reticulum; Membrane; Microsome;
KW   Mitochondrion; Reference proteome; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..271
FT                   /note="N-acetylaspartate synthetase"
FT                   /id="PRO_0000305232"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          115..258
FT                   /note="N-acetyltransferase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00532"
FT   REGION          1..38
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        13..28
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   271 AA;  30726 MW;  071F172B3192BC95 CRC64;
     MTYRGTRKSP CCSPPPRCGP PLPSGPAGSA LGPPSSGAEE EMTKEQVYLR EFQPADQEFA
     RRIFYEGIKE RILSSAFRGL KYQPLLQSVY AVIIIMCFVV TKSLLVTCCM PLFLLGMRYY
     YSRKIILNHL ECALRTDMSD IEQYYMKQPG SCFWVAVLEG KVVGIVAARG NEEDNVVELR
     RMSVDSNYRG KGIAKALGRK VLEFAMLNHY SSIVLGTTAV KIAAHKLYES LGFKHVGVVE
     HHIVPGMTHS LLERLFFQLR YHRYCLQLRE E