NAT8_ARATH
ID NAT8_ARATH Reviewed; 539 AA.
AC Q8VZQ5; Q9XIJ8;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Nucleobase-ascorbate transporter 8;
DE Short=AtNAT8;
GN Name=NAT8; OrderedLocusNames=At1g10540; ORFNames=T10O24.15, T10O24.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=16982705; DOI=10.1093/pcp/pcl011;
RA Maurino V.G., Grube E., Zielinski J., Schild A., Fischer K., Flugge U.-I.;
RT "Identification and expression analysis of twelve members of the
RT nucleobase-ascorbate transporter (NAT) gene family in Arabidopsis
RT thaliana.";
RL Plant Cell Physiol. 47:1381-1393(2006).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:16982705};
CC Multi-pass membrane protein {ECO:0000269|PubMed:16982705}.
CC -!- TISSUE SPECIFICITY: Highly expressed in ovules, endosperm and embryo.
CC {ECO:0000269|PubMed:16982705}.
CC -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC 2.A.40) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD39576.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007067; AAD39576.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28592.1; -; Genomic_DNA.
DR EMBL; AY063935; AAL36291.1; -; mRNA.
DR EMBL; AY096464; AAM20104.1; -; mRNA.
DR RefSeq; NP_172524.1; NM_100929.5.
DR AlphaFoldDB; Q8VZQ5; -.
DR SMR; Q8VZQ5; -.
DR STRING; 3702.AT1G10540.1; -.
DR PaxDb; Q8VZQ5; -.
DR PRIDE; Q8VZQ5; -.
DR ProteomicsDB; 251046; -.
DR EnsemblPlants; AT1G10540.1; AT1G10540.1; AT1G10540.
DR GeneID; 837595; -.
DR Gramene; AT1G10540.1; AT1G10540.1; AT1G10540.
DR KEGG; ath:AT1G10540; -.
DR Araport; AT1G10540; -.
DR TAIR; locus:2194631; AT1G10540.
DR eggNOG; KOG1292; Eukaryota.
DR HOGENOM; CLU_017959_5_3_1; -.
DR InParanoid; Q8VZQ5; -.
DR OMA; PQYFYQY; -.
DR OrthoDB; 387031at2759; -.
DR PhylomeDB; Q8VZQ5; -.
DR PRO; PR:Q8VZQ5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8VZQ5; baseline and differential.
DR Genevisible; Q8VZQ5; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0071702; P:organic substance transport; IEA:UniProt.
DR InterPro; IPR006043; NCS2.
DR Pfam; PF00860; Xan_ur_permease; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..539
FT /note="Nucleobase-ascorbate transporter 8"
FT /id="PRO_0000270165"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 368..388
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 399..421
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 433..453
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 470..490
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 539 AA; 59515 MW; A3ED84C5340CE015 CRC64;
MAGDGVENAK PPQKQEDLQP HPVKDQLYGI TYCLTSPPPW PETILLGFQH YLVMLGTTVL
IPTMLVSKID ARNEDKVKLI QTLLFVSGIN TLFQSFFGTR LPAVIGASYS YVPTTMSIVL
AARYNDIMDP QKRFEQIMRG IQGALIIASF LHILVGFSGL WRNVTRFLSP LSAVPLVAFS
GFGLYEQGFP MLAKCIEIGL PEIILLVIFS QYIPHLMQGE TCSNFFHRFA VIFSVVIVWL
YAYILTIGGA YSNTEINTQI SCRTDRAGII SASPWIRVPH PIQWGGAPTF NAGDIFAMMA
ASFVSLVEST GTYIAVSRYA SATPIPPSVL SRGIGWQGFG ILLCGLFGAG NATSVSVENA
GLLAVTRVGS RRVIQVAAGF MIFFSILGKF GAIFASIPAP IVAALYCLFF SYVGAGGLSL
IQFCNLNSFR TKFILGFSIF MGLSIPQYFY QYTTLETYGP VRTSATWFNN IINVPFSSKA
FVSGILAFFL DTTLPPKDKT TKKDRGLVWW KRFKSFQSDN RSEEFYSLPL NLSKYFPSH
