NAT9_HUMAN
ID NAT9_HUMAN Reviewed; 207 AA.
AC Q9BTE0; B2R7F0; Q9BTD0; Q9Y3T3;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Alpha/beta-tubulin-N-acetyltransferase 9 {ECO:0000305|PubMed:33479178};
DE EC=2.3.1.- {ECO:0000269|PubMed:33479178};
DE AltName: Full=Embryo brain-specific protein;
GN Name=NAT9; Synonyms=EBS;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Chen W., Zou X., Zhang J., Ci H.L., Li Y.P.;
RT "Molecular cloning and primary studies on EBS: a novel human embryo brain-
RT specific gene.";
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Adrenal gland;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Lung, and Muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=33479178; DOI=10.1073/pnas.2010140118;
RA Mok J.W., Choi K.W.;
RT "Novel function of N-acetyltransferase for microtubule stability and JNK
RT signaling in Drosophila organ development.";
RL Proc. Natl. Acad. Sci. U.S.A. 118:0-0(2021).
CC -!- FUNCTION: N-acetyltransferase that mediates the acetylation of the N-
CC terminal residues of alpha- and beta-tubulin.
CC {ECO:0000269|PubMed:33479178}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + N-terminal L-methionyl-[tubulin] = CoA + H(+) +
CC N-terminal N(alpha)-acetyl-L-methionyl-[tubulin];
CC Xref=Rhea:RHEA:69607, Rhea:RHEA-COMP:17729, Rhea:RHEA-COMP:17730,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC ChEBI:CHEBI:64731, ChEBI:CHEBI:133414;
CC Evidence={ECO:0000269|PubMed:33479178};
CC -!- INTERACTION:
CC Q9BTE0; O43324: EEF1E1; NbExp=4; IntAct=EBI-711919, EBI-1048486;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9BTE0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9BTE0-2; Sequence=VSP_025231;
CC -!- SIMILARITY: Belongs to the acetyltransferase family. GNAT subfamily.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY632082; AAU25819.1; -; mRNA.
DR EMBL; AK312958; BAG35797.1; -; mRNA.
DR EMBL; AL050269; CAB43370.1; -; mRNA.
DR EMBL; CH471099; EAW89197.1; -; Genomic_DNA.
DR EMBL; BC004195; AAH04195.1; -; mRNA.
DR EMBL; BC004225; AAH04225.1; -; mRNA.
DR CCDS; CCDS11706.1; -. [Q9BTE0-1]
DR CCDS; CCDS77102.1; -. [Q9BTE0-2]
DR PIR; T08699; T08699.
DR RefSeq; NP_001292006.1; NM_001305077.1. [Q9BTE0-2]
DR RefSeq; NP_001292007.1; NM_001305078.1. [Q9BTE0-1]
DR RefSeq; NP_001292008.1; NM_001305079.1.
DR RefSeq; NP_001292009.1; NM_001305080.1.
DR RefSeq; NP_001292010.1; NM_001305081.1.
DR RefSeq; NP_001292011.1; NM_001305082.1.
DR RefSeq; NP_001292012.1; NM_001305083.1.
DR RefSeq; NP_001292013.1; NM_001305084.1.
DR RefSeq; NP_001292014.1; NM_001305085.1.
DR RefSeq; NP_001292015.1; NM_001305086.1.
DR RefSeq; NP_001292016.1; NM_001305087.1.
DR RefSeq; NP_001292017.1; NM_001305088.1.
DR RefSeq; NP_056469.2; NM_015654.4. [Q9BTE0-1]
DR AlphaFoldDB; Q9BTE0; -.
DR SMR; Q9BTE0; -.
DR BioGRID; 117582; 19.
DR IntAct; Q9BTE0; 12.
DR MINT; Q9BTE0; -.
DR STRING; 9606.ENSP00000350467; -.
DR iPTMnet; Q9BTE0; -.
DR PhosphoSitePlus; Q9BTE0; -.
DR BioMuta; NAT9; -.
DR DMDM; 74752333; -.
DR EPD; Q9BTE0; -.
DR jPOST; Q9BTE0; -.
DR MassIVE; Q9BTE0; -.
DR MaxQB; Q9BTE0; -.
DR PaxDb; Q9BTE0; -.
DR PeptideAtlas; Q9BTE0; -.
DR PRIDE; Q9BTE0; -.
DR ProteomicsDB; 78979; -. [Q9BTE0-1]
DR ProteomicsDB; 78980; -. [Q9BTE0-2]
DR Antibodypedia; 19466; 68 antibodies from 19 providers.
DR DNASU; 26151; -.
DR Ensembl; ENST00000357814.8; ENSP00000350467.3; ENSG00000109065.12. [Q9BTE0-1]
DR Ensembl; ENST00000580301.5; ENSP00000462019.1; ENSG00000109065.12. [Q9BTE0-2]
DR GeneID; 26151; -.
DR KEGG; hsa:26151; -.
DR MANE-Select; ENST00000357814.8; ENSP00000350467.3; NM_015654.5; NP_056469.2.
DR UCSC; uc002jlq.3; human. [Q9BTE0-1]
DR CTD; 26151; -.
DR DisGeNET; 26151; -.
DR GeneCards; NAT9; -.
DR HGNC; HGNC:23133; NAT9.
DR HPA; ENSG00000109065; Low tissue specificity.
DR neXtProt; NX_Q9BTE0; -.
DR OpenTargets; ENSG00000109065; -.
DR PharmGKB; PA142671281; -.
DR VEuPathDB; HostDB:ENSG00000109065; -.
DR eggNOG; KOG4135; Eukaryota.
DR GeneTree; ENSGT00390000012745; -.
DR InParanoid; Q9BTE0; -.
DR OrthoDB; 1507385at2759; -.
DR PhylomeDB; Q9BTE0; -.
DR TreeFam; TF315021; -.
DR BioCyc; MetaCyc:ENSG00000109065-MON; -.
DR PathwayCommons; Q9BTE0; -.
DR SignaLink; Q9BTE0; -.
DR BioGRID-ORCS; 26151; 8 hits in 1020 CRISPR screens.
DR ChiTaRS; NAT9; human.
DR GeneWiki; NAT9; -.
DR GenomeRNAi; 26151; -.
DR Pharos; Q9BTE0; Tbio.
DR PRO; PR:Q9BTE0; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q9BTE0; protein.
DR Bgee; ENSG00000109065; Expressed in adenohypophysis and 195 other tissues.
DR ExpressionAtlas; Q9BTE0; baseline and differential.
DR Genevisible; Q9BTE0; HS.
DR GO; GO:0032991; C:protein-containing complex; IDA:LIFEdb.
DR GO; GO:0008080; F:N-acetyltransferase activity; IEA:InterPro.
DR GO; GO:0006473; P:protein acetylation; IBA:GO_Central.
DR InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR InterPro; IPR000182; GNAT_dom.
DR InterPro; IPR039135; NAT9-like.
DR PANTHER; PTHR13256; PTHR13256; 1.
DR Pfam; PF13302; Acetyltransf_3; 1.
DR SUPFAM; SSF55729; SSF55729; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Alternative splicing; Reference proteome; Transferase.
FT CHAIN 1..207
FT /note="Alpha/beta-tubulin-N-acetyltransferase 9"
FT /id="PRO_0000286874"
FT DOMAIN 35..180
FT /note="N-acetyltransferase"
FT VAR_SEQ 26
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334, ECO:0000303|PubMed:17974005,
FT ECO:0000303|Ref.1"
FT /id="VSP_025231"
FT VARIANT 56
FT /note="C -> R (in dbSNP:rs2305213)"
FT /id="VAR_032225"
FT CONFLICT 126
FT /note="L -> P (in Ref. 3; CAB43370)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 207 AA; 23361 MW; 1265AC8D2B2B376F CRC64;
MRLNQNTLLL GKKVVLVPYT SEHVPSRYHE WMKSEELQRL TASEPLTLEQ EYAMQCSWQE
DADKCTFIVL DAEKWQAQPG ATEESCMVGD VNLFLTDLED LTLGEIEVMI AEPSCRGKGL
GTEAVLAMLS YGVTTLGLTK FEAKIGQGNE PSIRMFQKLH FEQVATSSVF QEVTLRLTVS
ESEHQWLLEQ TSHVEEKPYR DGSAEPC
