NATB_BACSU
ID NATB_BACSU Reviewed; 386 AA.
AC P46904;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=ABC transporter permease protein NatB {ECO:0000305};
DE AltName: Full=ABC-type Na(+) transporter;
DE EC=7.2.2.4 {ECO:0000269|PubMed:9106203};
GN Name=natB {ECO:0000303|PubMed:9106203};
GN OrderedLocusNames=BSU02760 {ECO:0000312|EMBL:CAB12070.1};
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBUNIT,
RP AND INDUCTION.
RC STRAIN=BD99;
RX PubMed=9106203; DOI=10.1046/j.1365-2958.1997.2951656.x;
RA Cheng J., Guffanti A.A., Krulwich T.A.;
RT "A two-gene ABC-type transport system that extrudes Na+ in Bacillus
RT subtilis is induced by ethanol or protonophore.";
RL Mol. Microbiol. 23:1107-1120(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP INDUCTION.
RC STRAIN=168;
RX PubMed=17322186; DOI=10.1099/mic.0.2006/003673-0;
RA Ogura M., Tsukahara K., Hayashi K., Tanaka T.;
RT "The Bacillus subtilis NatK-NatR two-component system regulates expression
RT of the natAB operon encoding an ABC transporter for sodium ion extrusion.";
RL Microbiology 153:667-675(2007).
CC -!- FUNCTION: Part of an ABC transporter that catalyzes ATP-dependent
CC electrogenic sodium extrusion. {ECO:0000269|PubMed:9106203}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + Na(+)(in) = ADP + H(+) + Na(+)(out) + phosphate;
CC Xref=Rhea:RHEA:14633, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:456216; EC=7.2.2.4;
CC Evidence={ECO:0000269|PubMed:9106203};
CC -!- SUBUNIT: The complex is composed of NatA and NatB.
CC {ECO:0000269|PubMed:9106203}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Induced by ethanol and the protonophore carbonylcyanide p-
CC chlorophenylhydrazone (CCCP) and, more modestly, by sodium and
CC potassium. Positively regulated by the two-component regulatory system
CC NatK/NatR. {ECO:0000269|PubMed:17322186, ECO:0000269|PubMed:9106203}.
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DR EMBL; U30873; AAB53023.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12070.1; -; Genomic_DNA.
DR PIR; B69666; B69666.
DR RefSeq; NP_388158.1; NC_000964.3.
DR RefSeq; WP_003246346.1; NZ_JNCM01000030.1.
DR AlphaFoldDB; P46904; -.
DR SMR; P46904; -.
DR IntAct; P46904; 1.
DR STRING; 224308.BSU02760; -.
DR TCDB; 3.A.1.115.1; the atp-binding cassette (abc) superfamily.
DR PaxDb; P46904; -.
DR PRIDE; P46904; -.
DR EnsemblBacteria; CAB12070; CAB12070; BSU_02760.
DR GeneID; 938383; -.
DR KEGG; bsu:BSU02760; -.
DR PATRIC; fig|224308.179.peg.286; -.
DR eggNOG; COG1668; Bacteria.
DR InParanoid; P46904; -.
DR OMA; KDAFRDR; -.
DR PhylomeDB; P46904; -.
DR BioCyc; BSUB:BSU02760-MON; -.
DR BioCyc; MetaCyc:BSU02760-MON; -.
DR BRENDA; 7.2.2.4; 658.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0140679; F:ABC-type sodium transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0008554; F:P-type sodium transporter activity; IEA:RHEA.
DR InterPro; IPR032688; ABC2_membrane_2.
DR Pfam; PF12679; ABC2_membrane_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion transport; Membrane; Reference proteome; Sodium;
KW Sodium transport; Translocase; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..386
FT /note="ABC transporter permease protein NatB"
FT /id="PRO_0000096741"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 386 AA; 42793 MW; B4AA5B2687A478D9 CRC64;
MLSHIYKKEM IDALRDRKTI LLTILVPMIM MLGLVFFYES MLSDKGEQYT LAVGHSLPPA
LESKLNEIDE ISVKTFAKPE EAVDEGKADA YLNVPKEFDS YVNSMTPFKV DVYGNSIDQG
SSNAMQLVQS ALDQYKNEIV QQRLTNKHID QSVIQPFTIQ QKEADEEKGT SAIMLSAILP
MLILTSIVSG AMPIALDIMA GEKDRKSIEA LLLTPVSRNK VLVGKWLAVS TFGVASGVFA
LVFLILSTVL FTENLKTAFQ LGDHMWSVIG ASALIIVLSA LLISAMELFI SIMSSSVKEA
QSYMSLVVFL PVFPMFFIFS KAPNQFDLSY FLIPFLNLHA LFKQLLFGMV DPATILSTSG
TIAVLIAIFF LLARACFLKD KWVLPK
