NATK_BACSU
ID NATK_BACSU Reviewed; 318 AA.
AC P70954; O34965;
DT 10-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2003, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Sensor histidine kinase NatK {ECO:0000305};
DE EC=2.7.13.3 {ECO:0000250|UniProtKB:P0AEC8};
GN Name=natK {ECO:0000303|PubMed:17322186};
GN Synonyms=yccG {ECO:0000312|EMBL:BAA22234.1};
GN OrderedLocusNames=BSU02730 {ECO:0000312|EMBL:CAB12067.1};
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=BD99;
RX PubMed=9106203; DOI=10.1046/j.1365-2958.1997.2951656.x;
RA Cheng J., Guffanti A.A., Krulwich T.A.;
RT "A two-gene ABC-type transport system that extrudes Na+ in Bacillus
RT subtilis is induced by ethanol or protonophore.";
RL Mol. Microbiol. 23:1107-1120(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9274031; DOI=10.1099/00221287-143-8-2775;
RA Kumano M., Tamakoshi A., Yamane K.;
RT "A 32 kb nucleotide sequence from the region of the lincomycin-resistance
RT gene (22 degrees-25 degrees) of the Bacillus subtilis chromosome and
RT identification of the site of the lin-2 mutation.";
RL Microbiology 143:2775-2782(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP FUNCTION.
RX PubMed=11717295; DOI=10.1128/jb.183.24.7365-7370.2001;
RA Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T.,
RA Fujita Y.;
RT "Comprehensive DNA microarray analysis of Bacillus subtilis two-component
RT regulatory systems.";
RL J. Bacteriol. 183:7365-7370(2001).
RN [5]
RP FUNCTION.
RC STRAIN=168;
RX PubMed=17322186; DOI=10.1099/mic.0.2006/003673-0;
RA Ogura M., Tsukahara K., Hayashi K., Tanaka T.;
RT "The Bacillus subtilis NatK-NatR two-component system regulates expression
RT of the natAB operon encoding an ABC transporter for sodium ion extrusion.";
RL Microbiology 153:667-675(2007).
CC -!- FUNCTION: Member of the two-component regulatory system NatK/NatR that
CC positively regulates the expression of the natAB operon. Potentially
CC phosphorylates NatR. {ECO:0000269|PubMed:11717295,
CC ECO:0000269|PubMed:17322186}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3; Evidence={ECO:0000250|UniProtKB:P0AEC8};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
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DR EMBL; U30873; AAB53020.1; -; Genomic_DNA.
DR EMBL; AB000617; BAA22234.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12067.1; -; Genomic_DNA.
DR PIR; H69754; H69754.
DR RefSeq; NP_388155.1; NC_000964.3.
DR RefSeq; WP_003246454.1; NZ_JNCM01000030.1.
DR AlphaFoldDB; P70954; -.
DR SMR; P70954; -.
DR STRING; 224308.BSU02730; -.
DR PaxDb; P70954; -.
DR PRIDE; P70954; -.
DR DNASU; 938382; -.
DR EnsemblBacteria; CAB12067; CAB12067; BSU_02730.
DR GeneID; 938382; -.
DR KEGG; bsu:BSU02730; -.
DR PATRIC; fig|224308.179.peg.283; -.
DR eggNOG; COG3290; Bacteria.
DR OMA; FYISVTN; -.
DR PhylomeDB; P70954; -.
DR BioCyc; BSUB:BSU02730-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004673; F:protein histidine kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR032834; NatK_C.
DR Pfam; PF14501; HATPase_c_5; 1.
DR SMART; SM00387; HATPase_c; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell membrane; Kinase; Membrane; Nucleotide-binding;
KW Phosphoprotein; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix; Two-component regulatory system.
FT CHAIN 1..318
FT /note="Sensor histidine kinase NatK"
FT /id="PRO_0000074920"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 132..318
FT /note="Histidine kinase"
FT /evidence="ECO:0000305"
FT MOD_RES 134
FT /note="Phosphohistidine; by autocatalysis"
FT /evidence="ECO:0000305"
FT CONFLICT 164
FT /note="S -> N (in Ref. 1; AAB53020)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 318 AA; 35076 MW; 825FFFF993276254 CRC64;
MITLFQCLYL ILFSFICYQG AAAFSHSTAA SWLAAALGAA AAGLYIWNTK RVWKHCSSGL
CAWIAVIQVM SVGVVLIGTD IMPVLCVIAI FAGCEGLRIG QSALQARLSD QIDKLTQAEQ
HANQMLIDVR SRNHDTMKHI TAIKSAQPKA DTQAYIQNWA DQYSQYDRFL KGENAYVAGV
LYDFLEKARA SNVSVSLHMH TPLSSLPFSP ADQVSLVGNI LENALDSAAE AREKAEIKLE
TSLRSGLYVL TCENSTPGMD PKVLDTIYQS FGRSTKNGAH EGMGTYIIQK LVKGAFGRLD
FTYRHPIFRL EIKIPFQK
