NATR_BACSU
ID NATR_BACSU Reviewed; 233 AA.
AC P70955;
DT 10-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Transcriptional regulatory protein NatR {ECO:0000305};
GN Name=natR {ECO:0000303|PubMed:17322186};
GN Synonyms=yccH {ECO:0000312|EMBL:BAA22235.1};
GN OrderedLocusNames=BSU02740 {ECO:0000312|EMBL:CAB12068.1};
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=BD99;
RX PubMed=9106203; DOI=10.1046/j.1365-2958.1997.2951656.x;
RA Cheng J., Guffanti A.A., Krulwich T.A.;
RT "A two-gene ABC-type transport system that extrudes Na+ in Bacillus
RT subtilis is induced by ethanol or protonophore.";
RL Mol. Microbiol. 23:1107-1120(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9274031; DOI=10.1099/00221287-143-8-2775;
RA Kumano M., Tamakoshi A., Yamane K.;
RT "A 32 kb nucleotide sequence from the region of the lincomycin-resistance
RT gene (22 degrees-25 degrees) of the Bacillus subtilis chromosome and
RT identification of the site of the lin-2 mutation.";
RL Microbiology 143:2775-2782(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP FUNCTION.
RX PubMed=11717295; DOI=10.1128/jb.183.24.7365-7370.2001;
RA Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T.,
RA Fujita Y.;
RT "Comprehensive DNA microarray analysis of Bacillus subtilis two-component
RT regulatory systems.";
RL J. Bacteriol. 183:7365-7370(2001).
RN [5]
RP FUNCTION, AND DNA-BINDING.
RC STRAIN=168;
RX PubMed=17322186; DOI=10.1099/mic.0.2006/003673-0;
RA Ogura M., Tsukahara K., Hayashi K., Tanaka T.;
RT "The Bacillus subtilis NatK-NatR two-component system regulates expression
RT of the natAB operon encoding an ABC transporter for sodium ion extrusion.";
RL Microbiology 153:667-675(2007).
CC -!- FUNCTION: Member of the two-component regulatory system NatK/NatR that
CC positively regulates the expression of the natAB operon. Acts by
CC binding directly to the promoter of natAB.
CC {ECO:0000269|PubMed:11717295, ECO:0000269|PubMed:17322186}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- PTM: Phosphorylated by NatK. {ECO:0000305}.
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DR EMBL; U30873; AAB53021.1; -; Genomic_DNA.
DR EMBL; AB000617; BAA22235.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12068.1; -; Genomic_DNA.
DR PIR; A69755; A69755.
DR RefSeq; NP_388156.1; NC_000964.3.
DR RefSeq; WP_003234759.1; NZ_JNCM01000030.1.
DR AlphaFoldDB; P70955; -.
DR SMR; P70955; -.
DR STRING; 224308.BSU02740; -.
DR PaxDb; P70955; -.
DR PRIDE; P70955; -.
DR EnsemblBacteria; CAB12068; CAB12068; BSU_02740.
DR GeneID; 938379; -.
DR KEGG; bsu:BSU02740; -.
DR PATRIC; fig|224308.179.peg.284; -.
DR eggNOG; COG3279; Bacteria.
DR InParanoid; P70955; -.
DR OMA; ESDGNYS; -.
DR PhylomeDB; P70955; -.
DR BioCyc; BSUB:BSU02740-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR007492; LytTR_DNA-bd_dom.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF04397; LytTR; 1.
DR Pfam; PF00072; Response_reg; 1.
DR SMART; SM00850; LytTR; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS50930; HTH_LYTTR; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..233
FT /note="Transcriptional regulatory protein NatR"
FT /id="PRO_0000081392"
FT DOMAIN 3..117
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 129..233
FT /note="HTH LytTR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00112"
FT MOD_RES 54
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 233 AA; 26969 MW; 1A6B4C9B5C08C67F CRC64;
MVKVGLVDDY RVDLEKLEAI VSRMQDVEIV FSTDSAKEAY RRVKNGDIDL LLADIEMPHM
SGYELADLIK SHSLDVDVIF VTGHGGYAVH AFDLNVHDYI MKPYYADRLA ASFDRYLKKK
TETSLNGRIL IKQKSEMHVL QKKDIIFAER TGRSTTIVTT AEEVQTYQTL NDIKGDLPEK
DFLRSHRSFI INIHYIKHFS AYTKHSFTVS FEGTSKKAMI TKQQLDYFQN YYF
