ID   NB5R1_BOVIN             Reviewed;         305 AA.
AC   Q3MHW9;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=NADH-cytochrome b5 reductase 1;
DE            Short=b5R.1;
DE            EC=1.6.2.2;
GN   Name=CYB5R1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Ascending colon;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: NADH-cytochrome b5 reductases are involved in desaturation
CC       and elongation of fatty acids, cholesterol biosynthesis, drug
CC       metabolism, and, in erythrocyte, methemoglobin reduction.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] +
CC         H(+) + NAD(+); Xref=Rhea:RHEA:46680, Rhea:RHEA-COMP:10438, Rhea:RHEA-
CC         COMP:10439, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033, ChEBI:CHEBI:29034,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.6.2.2;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the flavoprotein pyridine nucleotide cytochrome
CC       reductase family. {ECO:0000305}.
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DR   EMBL; BC104584; AAI04585.1; -; mRNA.
DR   RefSeq; NP_001029690.1; NM_001034518.1.
DR   AlphaFoldDB; Q3MHW9; -.
DR   SMR; Q3MHW9; -.
DR   STRING; 9913.ENSBTAP00000026548; -.
DR   PaxDb; Q3MHW9; -.
DR   PRIDE; Q3MHW9; -.
DR   Ensembl; ENSBTAT00000026548; ENSBTAP00000026548; ENSBTAG00000019927.
DR   GeneID; 516287; -.
DR   KEGG; bta:516287; -.
DR   CTD; 51706; -.
DR   VEuPathDB; HostDB:ENSBTAG00000019927; -.
DR   VGNC; VGNC:97257; CYB5R1.
DR   eggNOG; KOG0534; Eukaryota.
DR   GeneTree; ENSGT00940000160784; -.
DR   InParanoid; Q3MHW9; -.
DR   OMA; QAQHPNR; -.
DR   OrthoDB; 1311668at2759; -.
DR   Proteomes; UP000009136; Chromosome 16.
DR   Bgee; ENSBTAG00000019927; Expressed in corpus luteum and 105 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004128; F:cytochrome-b5 reductase activity, acting on NAD(P)H; IEA:UniProtKB-EC.
DR   GO; GO:0071949; F:FAD binding; IBA:GO_Central.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.80; -; 1.
DR   InterPro; IPR001834; CBR-like.
DR   InterPro; IPR008333; Cbr1-like_FAD-bd_dom.
DR   InterPro; IPR017927; FAD-bd_FR_type.
DR   InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR   InterPro; IPR039261; FNR_nucleotide-bd.
DR   InterPro; IPR001433; OxRdtase_FAD/NAD-bd.
DR   InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR   PANTHER; PTHR19370; PTHR19370; 1.
DR   Pfam; PF00970; FAD_binding_6; 1.
DR   Pfam; PF00175; NAD_binding_1; 1.
DR   PRINTS; PR00371; FPNCR.
DR   SUPFAM; SSF52343; SSF52343; 1.
DR   SUPFAM; SSF63380; SSF63380; 1.
DR   PROSITE; PS51384; FAD_FR; 1.
PE   2: Evidence at transcript level;
KW   FAD; Flavoprotein; Lipid biosynthesis; Lipid metabolism; Membrane; NAD;
KW   Oxidoreductase; Reference proteome; Steroid biosynthesis;
KW   Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..305
FT                   /note="NADH-cytochrome b5 reductase 1"
FT                   /id="PRO_0000287544"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          44..156
FT                   /note="FAD-binding FR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00716"
FT   BINDING         136..166
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   BINDING         175..210
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   305 AA;  33861 MW;  BC12CA38311828F4 CRC64;
     MGLQPSPVLL ASLGVGLLTL LGVALGAYLV RRSRRPPVTL LDPNEKYQLR LLDKTTVNHN
     TKRFRFALPT AHHVLGLPVG KHVYLSARID GSLVIRPYTP VTSDEDQGYV DLVIKVYLKG
     VHPKFPEGGK MSQYLDSLKI GDVVEFRGPS GLLTYAGKGK FNIQPNKKAP PEARVARNLG
     MIAGGTGITP MLQLIRAILK DPEDPTQCFL LFANQTEKDI ILREDLEELQ ARHPGRFKLW
     FTLDHPPEGW AYSKGFVSVD MIREHLPAPG EDVLLLLCGP PPMVQLACHP SLDKLGYSPK
     MRFTY