ID   NB5R1_RAT               Reviewed;         305 AA.
AC   Q5EB81;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=NADH-cytochrome b5 reductase 1;
DE            Short=b5R.1;
DE            EC=1.6.2.2;
GN   Name=Cyb5r1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: NADH-cytochrome b5 reductases are involved in desaturation
CC       and elongation of fatty acids, cholesterol biosynthesis, drug
CC       metabolism, and, in erythrocyte, methemoglobin reduction.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] +
CC         H(+) + NAD(+); Xref=Rhea:RHEA:46680, Rhea:RHEA-COMP:10438, Rhea:RHEA-
CC         COMP:10439, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033, ChEBI:CHEBI:29034,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.6.2.2;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the flavoprotein pyridine nucleotide cytochrome
CC       reductase family. {ECO:0000305}.
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DR   EMBL; BC089945; AAH89945.1; -; mRNA.
DR   RefSeq; NP_001013144.1; NM_001013126.1.
DR   AlphaFoldDB; Q5EB81; -.
DR   SMR; Q5EB81; -.
DR   STRING; 10116.ENSRNOP00000057400; -.
DR   iPTMnet; Q5EB81; -.
DR   PhosphoSitePlus; Q5EB81; -.
DR   jPOST; Q5EB81; -.
DR   PaxDb; Q5EB81; -.
DR   PRIDE; Q5EB81; -.
DR   GeneID; 304805; -.
DR   KEGG; rno:304805; -.
DR   UCSC; RGD:1306084; rat.
DR   CTD; 51706; -.
DR   RGD; 1306084; Cyb5r1.
DR   eggNOG; KOG0534; Eukaryota.
DR   InParanoid; Q5EB81; -.
DR   OrthoDB; 1311668at2759; -.
DR   PhylomeDB; Q5EB81; -.
DR   Reactome; R-RNO-114608; Platelet degranulation.
DR   Reactome; R-RNO-1237044; Erythrocytes take up carbon dioxide and release oxygen.
DR   PRO; PR:Q5EB81; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004128; F:cytochrome-b5 reductase activity, acting on NAD(P)H; IEA:UniProtKB-EC.
DR   GO; GO:0071949; F:FAD binding; IBA:GO_Central.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.80; -; 1.
DR   InterPro; IPR001834; CBR-like.
DR   InterPro; IPR008333; Cbr1-like_FAD-bd_dom.
DR   InterPro; IPR017927; FAD-bd_FR_type.
DR   InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR   InterPro; IPR039261; FNR_nucleotide-bd.
DR   InterPro; IPR001433; OxRdtase_FAD/NAD-bd.
DR   InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR   PANTHER; PTHR19370; PTHR19370; 1.
DR   Pfam; PF00970; FAD_binding_6; 1.
DR   Pfam; PF00175; NAD_binding_1; 1.
DR   PRINTS; PR00371; FPNCR.
DR   SUPFAM; SSF52343; SSF52343; 1.
DR   SUPFAM; SSF63380; SSF63380; 1.
DR   PROSITE; PS51384; FAD_FR; 1.
PE   2: Evidence at transcript level;
KW   FAD; Flavoprotein; Lipid biosynthesis; Lipid metabolism; Membrane; NAD;
KW   Oxidoreductase; Reference proteome; Steroid biosynthesis;
KW   Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..305
FT                   /note="NADH-cytochrome b5 reductase 1"
FT                   /id="PRO_0000287547"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          44..156
FT                   /note="FAD-binding FR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00716"
FT   BINDING         136..166
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   BINDING         175..210
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   305 AA;  34223 MW;  759E91678EEB3D30 CRC64;
     MGIQPSPVLL ASLGVGLFTL FGLALGTYLV RRSRRPQVTL QDPDEKYLLR LLDKTTVSHN
     TRRFRFALPT AHHILGLPVG KHVYLSARID GSQVIRPYTP VTSDEDQGYV DLVIKVYLKG
     VHPKFSEGGK MSQYLDSLKI GDVVEFRGPS GLLSYAGKGN FNIQPNKKSP PELRVAKKLG
     MIAGGTGITP MLQLIRAILK VPEDPTQCFL LFANQTEKDI ILREDLEELQ AQYPIRFKLW
     FTLDYPPEDW TYSKGFVTAD MIQEHLPAPA EDVLLLLCGP PPMVQLACHP NLDKLGYSQK
     MRFTY