NBAS_DANRE
ID NBAS_DANRE Reviewed; 2372 AA.
AC Q5TYW4; A4QN83;
DT 26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=NBAS subunit of NRZ tethering complex {ECO:0000250|UniProtKB:A2RRP1};
DE AltName: Full=Neuroblastoma-amplified gene protein homolog {ECO:0000250|UniProtKB:A2RRP1};
DE AltName: Full=Neuroblastoma-amplified sequence {ECO:0000303|PubMed:21227923};
GN Name=nbas {ECO:0000250|UniProtKB:A2RRP1};
GN Synonyms=nag {ECO:0000303|PubMed:21227923}; ORFNames=si:ch211-214k9.1;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-369.
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=21227923; DOI=10.1093/nar/gkq1319;
RA Anastasaki C., Longman D., Capper A., Patton E.E., Caceres J.F.;
RT "Dhx34 and Nbas function in the NMD pathway and are required for embryonic
RT development in zebrafish.";
RL Nucleic Acids Res. 39:3686-3694(2011).
CC -!- FUNCTION: Involved in Golgi-to-endoplasmic reticulum (ER) retrograde
CC transport; the function is proposed to depend on its association in the
CC NRZ complex which is believed to play a role in SNARE assembly at the
CC ER (By similarity). Required for normal embryonic development
CC (PubMed:21227923). May play a role in the nonsense-mediated decay
CC pathway of mRNAs containing premature stop codons (PubMed:21227923).
CC {ECO:0000250|UniProtKB:A2RRP1, ECO:0000269|PubMed:21227923}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC {ECO:0000250|UniProtKB:A2RRP1}.
CC -!- DEVELOPMENTAL STAGE: Expressed prior to gastrulation and following
CC gastrulation cell movements at 10 hours post fertilization
CC (PubMed:21227923). Expressed ubiquitously during early development
CC (PubMed:21227923). {ECO:0000269|PubMed:21227923}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown leads to both intermediate
CC and severe phenotypes of abnormal embryonic development
CC (PubMed:21227923). Intermediate phenotypes show areas of necrosis in
CC the brain and neural structures with abnormal stacking of somites
CC (PubMed:21227923). Severe phenotypes show a small body with few
CC recognizable structures on top of the yolk sac with necrotic tissue
CC present in the anterior and posterior regions (PubMed:21227923).
CC Increase in premature stop codon containing slc24a5 mRNA transcript
CC levels in melanocytes (PubMed:21227923). {ECO:0000269|PubMed:21227923}.
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DR EMBL; BX649472; CAH68923.1; -; Genomic_DNA.
DR EMBL; BX649264; CAH68923.1; JOINED; Genomic_DNA.
DR EMBL; BX649299; CAH68923.1; JOINED; Genomic_DNA.
DR EMBL; BX649264; CAH68952.1; -; Genomic_DNA.
DR EMBL; BX649299; CAH68952.1; JOINED; Genomic_DNA.
DR EMBL; BX649472; CAH68952.1; JOINED; Genomic_DNA.
DR EMBL; BX649299; CAI11777.1; -; Genomic_DNA.
DR EMBL; BX649264; CAI11777.1; JOINED; Genomic_DNA.
DR EMBL; BX649472; CAI11777.1; JOINED; Genomic_DNA.
DR EMBL; BC135054; AAI35055.1; -; mRNA.
DR RefSeq; NP_001038272.1; NM_001044807.1.
DR AlphaFoldDB; Q5TYW4; -.
DR STRING; 7955.ENSDARP00000047833; -.
DR PaxDb; Q5TYW4; -.
DR PRIDE; Q5TYW4; -.
DR Ensembl; ENSDART00000047834; ENSDARP00000047833; ENSDARG00000008593.
DR GeneID; 556592; -.
DR KEGG; dre:556592; -.
DR CTD; 51594; -.
DR ZFIN; ZDB-GENE-041014-367; nbas.
DR eggNOG; KOG1797; Eukaryota.
DR GeneTree; ENSGT00390000012474; -.
DR HOGENOM; CLU_001315_0_0_1; -.
DR InParanoid; Q5TYW4; -.
DR OrthoDB; 11548at2759; -.
DR PhylomeDB; Q5TYW4; -.
DR TreeFam; TF313901; -.
DR Reactome; R-DRE-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR PRO; PR:Q5TYW4; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 20.
DR Bgee; ENSDARG00000008593; Expressed in tail and 20 other tissues.
DR ExpressionAtlas; Q5TYW4; baseline.
DR GO; GO:0070939; C:Dsl1/NZR complex; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0043009; P:chordate embryonic development; IMP:ZFIN.
DR GO; GO:0048703; P:embryonic viscerocranium morphogenesis; IMP:ZFIN.
DR GO; GO:2000623; P:negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IMP:UniProtKB.
DR GO; GO:0000956; P:nuclear-transcribed mRNA catabolic process; IMP:UniProtKB.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IMP:ZFIN.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR029145; NBAS_N.
DR InterPro; IPR013244; Sec39_domain.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF15492; Nbas_N; 1.
DR Pfam; PF08314; Sec39; 1.
DR SMART; SM00320; WD40; 2.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Endoplasmic reticulum; Protein transport; Reference proteome;
KW Repeat; Transport; WD repeat.
FT CHAIN 1..2372
FT /note="NBAS subunit of NRZ tethering complex"
FT /id="PRO_0000292807"
FT REPEAT 119..158
FT /note="WD 1"
FT REPEAT 304..343
FT /note="WD 2"
FT REGION 447..468
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 629..668
FT /evidence="ECO:0000255"
FT CONFLICT 59
FT /note="C -> WYS (in Ref. 2; AAI35055)"
FT /evidence="ECO:0000305"
FT CONFLICT 226
FT /note="N -> S (in Ref. 2; AAI35055)"
FT /evidence="ECO:0000305"
FT CONFLICT 233
FT /note="Y -> H (in Ref. 2; AAI35055)"
FT /evidence="ECO:0000305"
FT CONFLICT 288
FT /note="R -> Q (in Ref. 2; AAI35055)"
FT /evidence="ECO:0000305"
FT CONFLICT 320
FT /note="I -> V (in Ref. 2; AAI35055)"
FT /evidence="ECO:0000305"
FT CONFLICT 366
FT /note="R -> K (in Ref. 2; AAI35055)"
FT /evidence="ECO:0000305"
FT CONFLICT 369
FT /note="I -> K (in Ref. 2; AAI35055)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2372 AA; 267981 MW; C572B7FB965D0F04 CRC64;
MAGDIILEDE EENILYDLLV NTEWPPETDT QLRGLKEHNS SLVAKAVTGP FRFFLHYLCP
SSSTLPPGLV RLATKQVNWQ LVLASNGKLL AVVQDQCVEI RSARDDFGSI IGKCQVPKDP
NPHWRRVAWS HDCALLAYAD STGTVRVFDL MGSELFIIPP AMSFPGDFSY AAAGLMFLEY
TGSAQWSAEL LVITYSGQLK SYLVSVGTNQ GFQENHTFSF SAHYSNGITT AIYHPGHRLL
LVGGCESGDS DVSRASQCGI TAWRVLSGLP HYKQVTSYED DISSSQRRGF FKMPSFRLFS
RHNGEQDGVF RMSLSPDGTI LAVIHFSGSL SLWDIPSFKL RGSWKQEEQP GFDEINPEWK
TSLEKRKKIK DKEQYQSLLD VSWWSETALI LARCSGALTV SSVRTLKNLL GKSCEWFEPS
PRVTSAHDGG FLSLECEVKL AHKRSRLESS VKGEEDDGDD DSDSDEEASA KARYFGYVKQ
GLYYVTEMER FAPPRKRPRT VIKNYRLVSL RSTTPEELYQ RKIDNEEYGE ALSLAQAYGL
DSDLVYQRQW RKSTVSIASI QDYLSKIKKR SWVLHECVER VPENVDAAKE LLQYGLKGTD
LEALIAIGAR EDQGRFILSG DLDMDDAPYE DFLSMEEELE QRKERESKKR QELLKKVDFS
KLTLEQKELC RSRLKLLCYL DRLATYEEIL GGPHAAEQRF DGEFFKKFRN QNIVLSARTY
ARESNVQALD ILFTYHGAEL LQHRLAILCN FPETTSPHEY SDLLPKAGVD KEGNLVLIPW
EESRHRDLDW CEVLECREVV EPKPMDDCQF LYEEQPELER FRSADPFITL LTEWYLTRAQ
DIESHSRQVD CSLSLVRLGK EQNIPGLERL CDDLVTMETL VYETSCDLSV TLKELQQLRD
IDKLRLLMKN SSEDRYVRNC FQWMVPFLHR CEGQRVGSAS SLLREYLVTL AKDDLTLPLK
LFQHSKPDCH PKIIGDSDQL MTVALKCIYS CERDDQLALC YDVLECLPQR GYGPETDTTK
ALHDQVDTLE KHLSVAEVLE KHGLQKPISF VRNSQNSKEE AHQLMVRLTR HTGRKNPPVS
ETVWRSLLQD LLDMQQNVYT CLEPETCHQV FVESLLCSSR EENVRLAGQL MHCSGVSEDT
PVSVSLRGKA HARVSYSRSV ELVLAAAREY FNSSATLSDP CMSLARSCLQ LITDCPPLVQ
EELDLITALS RLEQFGVKIL PLQVRLRTDR LSLIKECISQ CPTAYRQSLL LLSLARLLRV
AGDDEAKRKG QVLTLLAEQA LLCQDFKASY IHCQELMAAG YSEGWDVCAQ LGQCETFTQL
SGRQELMAFS LTHCPPSRIQ TLLAASSSLQ TQVLYQAVNY KIDPSQARRT GSELFEIKVL
DDENVTEIPT KLYSVGAAVP GSINPTDLLH RTTARTIEVL TNTTMSTKAV LTTVIDSHWW
RESLSLLRPL HGPAVDRNRA GAANQNADLE KQGCSPFYEE LFDDPYVNTS EDVYSSYHYN
PQEDFAEVLL RTGKLAETNS EGQNLFPATE VLLQLASDAF PKDMTLALAY LLALPQVLDA
NTCFEKQPPS ALSLHLAAYY YSLQIYSRLA PCFKDKCSPL YRADPRALIR LVTKHVTDHA
GCDWPDEDLE ALIGQLRLYS ERLTDFTQAQ VLQGLGRGVD VQRFSSDNQY KKETILGLAE
TLDENVYSIS RSLAHRYSIP LWEVHMTHLE FLFTDSGLST KDIEGRTEAL GLFETLKTDP
ESFYMHMTKY VYPSIAGTDQ LRLLFYFTLL ENCGCANYFS QTAMKPDTHL KLLKKLKAVA
VGLDYKKMTE ENSDPLKALE PILTSQNVLS ISKLASRIPQ KGGEALTSSA VHSTWLHKLF
WEGDQHVLKK APQTDQDFLH AYDTCAKYFD RLLPVDFVNF LDAITFSPEA ANKLSVGTRL
EITKRAQKAL KSISDKTKKK GNDDRSENSA FNFDTALTHV QQSLTHLETL SHSFLLSLKT
SQEELLQKYS RIYDLSRSEQ SKVHHLAVTM ATDGQPLERI EQLLSVAVGT SDLSPKSVVQ
DAVERIISAL SGDKDVLKDY ADPLKVLEGI VAVVHTNVQS GGNIVTSEDM LEWLRPFCGD
DTKPVRPRIE VLQILEHSFN LSDQDIHLLV YFRSQAILKA CWPNRKCEME DVENEKKRNS
LFLELLESSS KWEEFQHLML LLQAWPPVTD KSRLETDQNP WVCLTSTLLV HSSSVSQINI
GNEVLSMCRS LYLTKHKLNP QGIGNISSLL LNAGLKLPAL KLMTESKDEH LLKLTQDQIR
NITEVTEEVC DTELLSLLLN AGMLISCVDT AVYPSLVSHM LAQGDWDVEK AAYDLQQAGH
SAQAGSLLLS YRGSHPGQFT FNSALAVIRK WL
