NBDY_HUMAN
ID NBDY_HUMAN Reviewed; 68 AA.
AC A0A0U1RRE5;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 17-FEB-2016, sequence version 1.
DT 03-AUG-2022, entry version 34.
DE RecName: Full=Negative regulator of P-body association {ECO:0000312|HGNC:HGNC:50713};
DE AltName: Full=P-body dissociating protein {ECO:0000303|PubMed:27918561};
DE AltName: Full=Protein NoBody {ECO:0000303|PubMed:27918561};
GN Name=NBDY {ECO:0000312|HGNC:HGNC:50713};
GN Synonyms=LINC01420 {ECO:0000312|HGNC:HGNC:50713};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1] {ECO:0000312|Proteomes:UP000005640}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15772651; DOI=10.1038/nature03440;
RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA Rogers J., Bentley D.R.;
RT "The DNA sequence of the human X chromosome.";
RL Nature 434:325-337(2005).
RN [2] {ECO:0000312|EMBL:EAW93234.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP FUNCTION, INTERACTION WITH DCP1A; DCP2 AND EDC4, SUBCELLULAR LOCATION,
RP MUTAGENESIS OF LYS-22; PRO-23; PRO-24; LYS-25; LYS-26; ARG-27; CYS-28;
RP LEU-29; LEU-30; PRO-32; ARG-33; TRP-34; ASP-35; TYR-36; PRO-37; GLU-38;
RP GLY-39; THR-40 AND PRO-41, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=27918561; DOI=10.1038/nchembio.2249;
RA D'Lima N.G., Ma J., Winkler L., Chu Q., Loh K.H., Corpuz E.O., Budnik B.A.,
RA Lykke-Andersen J., Saghatelian A., Slavoff S.A.;
RT "A human microprotein that interacts with the mRNA decapping complex.";
RL Nat. Chem. Biol. 13:174-180(2017).
CC -!- FUNCTION: Promotes dispersal of P-body components and is likely to play
CC a role in the mRNA decapping process. {ECO:0000269|PubMed:27918561}.
CC -!- SUBUNIT: Interacts with mRNA decapping proteins DCP1A, DCP2 and EDC4.
CC {ECO:0000269|PubMed:27918561}.
CC -!- INTERACTION:
CC A0A0U1RRE5; Q9NPI6: DCP1A; NbExp=4; IntAct=EBI-27058088, EBI-374238;
CC A0A0U1RRE5; Q6P2E9: EDC4; NbExp=11; IntAct=EBI-27058088, EBI-1006038;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, P-body {ECO:0000269|PubMed:27918561}.
CC Note=Localizes to P-bodies at low concentrations without dissociating
CC them. {ECO:0000269|PubMed:27918561}.
CC -!- CAUTION: Was previously thought to be non-coding but has been shown to
CC be translated and functional. {ECO:0000269|PubMed:27918561}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL158200; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL354865; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; KF459114; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471154; EAW93234.1; -; Genomic_DNA.
DR CCDS; CCDS87751.1; -.
DR RefSeq; NP_001335058.1; NM_001348129.1.
DR AlphaFoldDB; A0A0U1RRE5; -.
DR IntAct; A0A0U1RRE5; 17.
DR BioMuta; NBDY; -.
DR EPD; A0A0U1RRE5; -.
DR jPOST; A0A0U1RRE5; -.
DR MassIVE; A0A0U1RRE5; -.
DR MaxQB; A0A0U1RRE5; -.
DR PeptideAtlas; A0A0U1RRE5; -.
DR DNASU; 550643; -.
DR Ensembl; ENST00000374922.9; ENSP00000489583.1; ENSG00000204272.13.
DR Ensembl; ENST00000423617.2; ENSP00000489486.1; ENSG00000204272.13.
DR Ensembl; ENST00000637096.1; ENSP00000490217.1; ENSG00000204272.13.
DR GeneID; 550643; -.
DR KEGG; hsa:550643; -.
DR MANE-Select; ENST00000374922.9; ENSP00000489583.1; NM_001348129.2; NP_001335058.1.
DR CTD; 550643; -.
DR DisGeNET; 550643; -.
DR GeneCards; NBDY; -.
DR HGNC; HGNC:50713; NBDY.
DR HPA; ENSG00000204272; Low tissue specificity.
DR MIM; 300992; gene.
DR neXtProt; NX_A0A0U1RRE5; -.
DR OpenTargets; ENSG00000204272; -.
DR VEuPathDB; HostDB:ENSG00000204272; -.
DR GeneTree; ENSGT00390000004891; -.
DR OMA; NTRETRP; -.
DR OrthoDB; 1629244at2759; -.
DR PathwayCommons; A0A0U1RRE5; -.
DR SignaLink; A0A0U1RRE5; -.
DR BioGRID-ORCS; 550643; 0 hits in 3 CRISPR screens.
DR ChiTaRS; NBDY; human.
DR Pharos; A0A0U1RRE5; Tbio.
DR PRO; PR:A0A0U1RRE5; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; A0A0U1RRE5; protein.
DR Bgee; ENSG00000204272; Expressed in kidney epithelium and 188 other tissues.
DR ExpressionAtlas; A0A0U1RRE5; baseline and differential.
DR GO; GO:0000932; C:P-body; IDA:UniProtKB.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0010607; P:negative regulation of cytoplasmic mRNA processing body assembly; IDA:UniProtKB.
DR GO; GO:0000956; P:nuclear-transcribed mRNA catabolic process; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW Cytoplasm; mRNA processing; Reference proteome.
FT CHAIN 1..68
FT /note="Negative regulator of P-body association"
FT /id="PRO_0000439205"
FT REGION 1..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 30
FT /note="Required for interaction with EDC4"
FT /evidence="ECO:0000269|PubMed:27918561"
FT SITE 34
FT /note="Required for interaction with EDC4"
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 22
FT /note="K->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 23
FT /note="P->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 24
FT /note="P->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 25
FT /note="K->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 26
FT /note="K->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 27
FT /note="R->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 28
FT /note="C->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 29
FT /note="L->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 30
FT /note="L->A: Impaired interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 32
FT /note="P->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 33
FT /note="R->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 34
FT /note="W->A: Impaired interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 35
FT /note="D->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 36
FT /note="Y->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 37
FT /note="P->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 38
FT /note="E->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 39
FT /note="G->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 40
FT /note="T->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
FT MUTAGEN 41
FT /note="P->A: No effect on interaction with EDC4."
FT /evidence="ECO:0000269|PubMed:27918561"
SQ SEQUENCE 68 AA; 7025 MW; FA372C7380B65853 CRC64;
MGDQPCASGR STLPPGNARE AKPPKKRCLL APRWDYPEGT PNGGSTTLPS APPPASAGLK
SHPPPPEK
