NBEL2_MOUSE
ID NBEL2_MOUSE Reviewed; 2742 AA.
AC Q6ZQA0;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 2.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Neurobeachin-like protein 2;
GN Name=Nbeal2; Synonyms=Kiaa0540;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1362-2742 (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [3]
RP SEQUENCE REVISION.
RC TISSUE=Brain;
RA Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.;
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2727, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Kidney, Liver, Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Probably involved in thrombopoiesis. Plays a role in the
CC development or secretion of alpha-granules, that contain several growth
CC factors important for platelet biogenesis (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6ZQA0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6ZQA0-2; Sequence=VSP_033508;
CC -!- SIMILARITY: Belongs to the WD repeat neurobeachin family.
CC {ECO:0000305}.
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DR EMBL; AC132103; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK129158; BAC97968.2; -; mRNA.
DR SMR; Q6ZQA0; -.
DR STRING; 10090.ENSMUSP00000128586; -.
DR iPTMnet; Q6ZQA0; -.
DR PhosphoSitePlus; Q6ZQA0; -.
DR EPD; Q6ZQA0; -.
DR jPOST; Q6ZQA0; -.
DR MaxQB; Q6ZQA0; -.
DR PaxDb; Q6ZQA0; -.
DR PRIDE; Q6ZQA0; -.
DR ProteomicsDB; 252786; -. [Q6ZQA0-1]
DR ProteomicsDB; 252787; -. [Q6ZQA0-2]
DR Antibodypedia; 62670; 48 antibodies from 12 providers.
DR Ensembl; ENSMUST00000133191; ENSMUSP00000121373; ENSMUSG00000056724. [Q6ZQA0-2]
DR MGI; MGI:2448554; Nbeal2.
DR VEuPathDB; HostDB:ENSMUSG00000056724; -.
DR eggNOG; KOG1787; Eukaryota.
DR GeneTree; ENSGT00940000158454; -.
DR InParanoid; Q6ZQA0; -.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR ChiTaRS; Nbeal2; mouse.
DR PRO; PR:Q6ZQA0; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q6ZQA0; protein.
DR Bgee; ENSMUSG00000056724; Expressed in granulocyte and 121 other tissues.
DR ExpressionAtlas; Q6ZQA0; baseline and differential.
DR Genevisible; Q6ZQA0; MM.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0019901; F:protein kinase binding; IBA:GO_Central.
DR GO; GO:0007596; P:blood coagulation; IMP:MGI.
DR GO; GO:0035855; P:megakaryocyte development; IMP:MGI.
DR GO; GO:0070889; P:platelet alpha granule organization; IMP:MGI.
DR GO; GO:0030220; P:platelet formation; ISS:UniProtKB.
DR GO; GO:0008104; P:protein localization; IBA:GO_Central.
DR GO; GO:0042060; P:wound healing; IMP:MGI.
DR CDD; cd06071; Beach; 1.
DR CDD; cd01201; PH_BEACH; 1.
DR Gene3D; 1.10.1540.10; -; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000409; BEACH_dom.
DR InterPro; IPR036372; BEACH_dom_sf.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR031570; NBEA/BDCP_DUF4704.
DR InterPro; IPR026916; NBEAL2.
DR InterPro; IPR023362; PH-BEACH_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR13743:SF111; PTHR13743:SF111; 1.
DR Pfam; PF02138; Beach; 1.
DR Pfam; PF15787; DUF4704; 1.
DR Pfam; PF14844; PH_BEACH; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM01026; Beach; 1.
DR SMART; SM00320; WD40; 3.
DR SUPFAM; SSF48371; SSF48371; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF81837; SSF81837; 1.
DR PROSITE; PS50197; BEACH; 1.
DR PROSITE; PS51783; PH_BEACH; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Endoplasmic reticulum; Phosphoprotein;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..2742
FT /note="Neurobeachin-like protein 2"
FT /id="PRO_0000333255"
FT DOMAIN 1903..2028
FT /note="BEACH-type PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01119"
FT DOMAIN 2041..2333
FT /note="BEACH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00026"
FT REPEAT 2374..2412
FT /note="WD 1"
FT REPEAT 2436..2479
FT /note="WD 2"
FT REPEAT 2482..2519
FT /note="WD 3"
FT REPEAT 2532..2570
FT /note="WD 4"
FT REPEAT 2577..2619
FT /note="WD 5"
FT REPEAT 2627..2662
FT /note="WD 6"
FT REPEAT 2670..2705
FT /note="WD 7"
FT REGION 1312..1333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1356..1434
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1364..1383
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1397..1434
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1855
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q6ZNJ1"
FT MOD_RES 2727
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 2730
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6ZNJ1"
FT VAR_SEQ 1896
FT /note="M -> ME (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14621295"
FT /id="VSP_033508"
SQ SEQUENCE 2742 AA; 301924 MW; C1345C9E4A40204A CRC64;
MAASERLYEL WLLYYAQKDL GYLQQWLKAF VGVFEKTISL SSLEPRRPEE AGAEVPLLPL
DALHALAEQL DQDDLDQALL LLKLFIILCR NLENVEAGWG QVLVPRVLAL LTVLMAELKG
SSQESHGTQL ENVALHALLL CEGLFDPYQT WRRQLTGEVI SSKEKSKYKF PPAALPCEFG
AFFQENLQDA ERLPPTLLLR LIHLFGAILA GGKANGQMAV SAGSVQGLLG VVRGWGRGPA
QDPQQVPLAL RALVGAVHVL HASRAPPRGP ELRTLLEGYF HILNADWPTS PSSSPEEALV
TLRVSMLDAI PMMLACEDRP VLQATFLSNN CFEHLIRLIQ NSKLYLQARA PPEGDSDLAT
WLLTEPDVQK VLDQDTDAIA VHVVRVLTCI MSGSPSAKEV FKERIGYQHL QEVLQSHGPP
THRLLQELLN MAVEGDHSMH PPPPIRNEQP VLVLMQWLPA LPTAELRLFL AQRLWWLCDS
CPASRTTCVQ AGLVGYLLET LNTGTALGAR CQEQLLALLQ ALGRVSLRPL ELRRLLRPPP
GLDSESSGNE SQKARHAGAV IRALSGMARH RGPARALRYF DLTPSMAGIM VPPVQRWPGA
GFTFHAWLCL QSSEAVPTSA PSRPLQRKQL YSFFTSSGSG FEAFFTAAGT LVVAVCTRKE
YVTVNLPEVS FADSAWHCVA IVHVPGRRPF SQNLVNVFKD GHLVKTVPFR FPSLSEPFSS
CCIGSAGHRT TTTTTGLPAS SVSTALAHTH PSLTRSQSVP ASTGLGWGPG LGTPLQEGSV
SSTLAGTQDT RWGSPTSLEG ELGAVAIFHE ALQPSALRVL CSLGPNEPAP FKPEGELHEF
GTKLLLHYSP QACKNNICLD LSPGHGLDGR LTGHRVETWD VKDVVNCVGG MGVLLPLLER
VAVQPQEAEA GPCETHDLVG PELTSGRNTQ GLLLPLGKSS EDRMERNAVA AFLLMLRNFL
QNHTVNQESL VQCQGPAIIG ALLRKVPSSA MDMNVLMSAQ LLMEQAAADG GGPLLYLLYQ
HLLFNFHLWT LSDFAVRLGH IQYMSSMVRE HRQKLRKKYG VQFLLDALRT HYSPQRERPL
AADDLRTVQT SLLGLVREFL VRNFSVEDMQ VVLNFLAATG DDGQVVGTLE LLLTLLQGSP
VQDPLAAFLL ELGNLEVLLA LLVRPKSTPL LTDRVCKILR RLQQNERLPE RNRQRIRLHD
CGLQGLVASL SEESISPQLC QGLYKLFVGT DCLNLSDLLA VVQLSLQADL SVRLDICRQL
FYLIYGQPDV VRLLARQAGW QDVLTRLYVL EAATDSSPPR FLPELPISSE LALSPPPTEL
PADSSDVFLP SESPCPDQDA FYQALSPFST PFDLGLERAS IGSGNTAGGG SSNGTVTPAS
QPGTPSPLDG PRPFPTAQGR HSSSLSNVLE DGSLLEPNIS GDDTSNTSNP QQTPEEELCN
LLTNVLFSVT WRGVEGSAEA AWRERGQVFS VLTQLGASAT LVRPPDCIKR SLLEMMLESA
LTDIKEAPPG GLANLSQQAL WLLRLLQDFL CAEGHGNQEL WSEKLFEGVC NLLDRLGAWP
HLANSTADLR EMAQIGLRLV LGYILLEDPQ LHAQAYVKLH TLLQTAVPTR REEACYVLSK
LEAALSRALT TSSSETEHAS TAVAASERCS WLVPLVRTLL DRAYGPLGLQ WGLPSLPPTN
GSPTFFEDFQ AFCATAEWRH FIDKQVQPTM SKFEMDTYAK SHDLMSGFWN ACYDTLMSSG
QRHQRDRIQS RRAFKELVLE PAQRRARVEG LRYASVLKQQ AAQHSTALLH WGALWRQLSS
PCGAWALRIP PAPHWKLSSA ETYSRMRLKL VPNHHFDPHL EASALRDNLG EAPMTPTEET
SLPLAVTKEA KISAPPEELP EEQLGEEDLA ALESLMEAAE LDEKREKLVL SAECQLVTVV
AVVPGLLEIT TQHVYFYDGS TERVETEEGI GHDFRRPLAQ LREVHLRRFN LRRSALELFF
IDQSNYFLNF PHKVAASSAS SPCQAPRPQL YPIPSHTQLR NQVYSLLLRL RPPTQGYLSS
RSPLEMLRAS GLTQKWVQRE ISNFEYLMQL NTIAGRTYND LSQYPVFPWV LQDYVSPVLD
LSNPAVFRDL SKPIGVVNPK HAQLVREKYE SFEDPAGTID KFHYGTHYSN AAGVMHYLIR
VEPFTSLHVQ LQSGRFDCSD RQFHSVAAAW QARLESPADV KELIPEFFYF PDFLENQNGF
DLGCLQLTNE KVGDVVLPPW AGSPEDFIQK HRQALESEYV STHLHEWIDL IFGYKQRGPA
AEEALNVFYY CTYEGAVDLD HVADERERKA LEGIISNFGQ TPCQLLKEPH PPRLSAEEAA
NRLARLDTNS PSIFQNLNQL KAFFAEVVSE AVPLVLALVP HRQSHSFITQ SSSDMLVTVS
ASGLLGTHTW LPYDRNINNY FTFSKDPTMG SPKVQKLLSG PWVSDSGVSA QALAVAPDGK
LLFSGGHWDG SLRVTSLPRG RLLNQLSRHL DIVTCLALDT CGIYLISGSR DTTCMVWRLL
QQSGLSAGLA PKPVQVLYGH VAAVSCVAIS TELDMAVSGS EDGTVIIHTV RRGQFVAALR
PPGATLPGPI SHLALGAEGQ IVVQSSACER PGAQVTYSLH LYSVNGRLRA SVTLTEQPTA
LTVAEDFVLL GTAQCSLHIL HLNKLRPAVP PLPMKVPVHS VSVTKERSHV LVGLEDGKLI
VVGAGQPSEV RSSQFARRLW RSSRRISQVS SGETEYNPGE AR
