NBL1_MOUSE
ID NBL1_MOUSE Reviewed; 178 AA.
AC Q61477; Q499K5;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Neuroblastoma suppressor of tumorigenicity 1;
DE AltName: Full=N03;
DE AltName: Full=Zinc finger protein DAN;
DE Flags: Precursor;
GN Name=Nbl1; Synonyms=Dan, Dana;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8609050; DOI=10.1111/j.1349-7006.1996.tb00200.x;
RA Ozaki T., Ma J., Takenaga K., Sakiyama S.;
RT "Cloning of mouse DAN cDNA and its down-regulation in transformed cells.";
RL Jpn. J. Cancer Res. 87:58-61(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Amnion, and Inner ear;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=23063586; DOI=10.1016/j.jmb.2012.10.003;
RA Kattamuri C., Luedeke D.M., Nolan K., Rankin S.A., Greis K.D., Zorn A.M.,
RA Thompson T.B.;
RT "Members of the DAN family are BMP antagonists that form highly stable
RT noncovalent dimers.";
RL J. Mol. Biol. 424:313-327(2012).
CC -!- FUNCTION: Possible candidate as a tumor suppressor gene of
CC neuroblastoma. May play an important role in preventing cells from
CC entering the final stage (G1/S) of the transformation process.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:23063586}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23063586}.
CC -!- SIMILARITY: Belongs to the DAN family. {ECO:0000305}.
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DR EMBL; D50263; BAA08817.1; -; mRNA.
DR EMBL; AK133787; BAE21841.1; -; mRNA.
DR EMBL; AK146535; BAE27241.1; -; mRNA.
DR EMBL; AK158008; BAE34315.1; -; mRNA.
DR EMBL; AL805923; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466615; EDL13294.1; -; Genomic_DNA.
DR EMBL; BC099856; AAH99856.1; -; mRNA.
DR EMBL; BC099857; AAH99857.1; -; mRNA.
DR EMBL; BC099858; AAH99858.1; -; mRNA.
DR EMBL; BC107810; AAI07811.1; -; mRNA.
DR CCDS; CCDS18839.1; -.
DR RefSeq; NP_032701.2; NM_008675.2.
DR RefSeq; XP_006538688.1; XM_006538625.3.
DR RefSeq; XP_006538689.1; XM_006538626.3.
DR AlphaFoldDB; Q61477; -.
DR SMR; Q61477; -.
DR MINT; Q61477; -.
DR STRING; 10090.ENSMUSP00000045608; -.
DR GlyConnect; 2544; 1 N-Linked glycan (1 site).
DR GlyGen; Q61477; 1 site, 1 N-linked glycan (1 site).
DR PhosphoSitePlus; Q61477; -.
DR CPTAC; non-CPTAC-3393; -.
DR MaxQB; Q61477; -.
DR PaxDb; Q61477; -.
DR PeptideAtlas; Q61477; -.
DR PRIDE; Q61477; -.
DR ProteomicsDB; 252788; -.
DR Antibodypedia; 29695; 313 antibodies from 33 providers.
DR DNASU; 17965; -.
DR Ensembl; ENSMUST00000042844; ENSMUSP00000045608; ENSMUSG00000041120.
DR GeneID; 17965; -.
DR KEGG; mmu:17965; -.
DR UCSC; uc008vls.1; mouse.
DR CTD; 4681; -.
DR MGI; MGI:104591; Nbl1.
DR VEuPathDB; HostDB:ENSMUSG00000041120; -.
DR eggNOG; ENOG502RYP0; Eukaryota.
DR GeneTree; ENSGT00940000154209; -.
DR HOGENOM; CLU_115450_0_0_1; -.
DR InParanoid; Q61477; -.
DR OMA; GLNVYVQ; -.
DR OrthoDB; 1517793at2759; -.
DR PhylomeDB; Q61477; -.
DR TreeFam; TF106445; -.
DR BioGRID-ORCS; 17965; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Nbl1; mouse.
DR PRO; PR:Q61477; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q61477; protein.
DR Bgee; ENSMUSG00000041120; Expressed in lip and 218 other tissues.
DR Genevisible; Q61477; MM.
DR GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR GO; GO:0036122; F:BMP binding; IDA:BHF-UCL.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0016015; F:morphogen activity; IDA:BHF-UCL.
DR GO; GO:0048018; F:receptor ligand activity; IBA:GO_Central.
DR GO; GO:0009887; P:animal organ morphogenesis; IBA:GO_Central.
DR GO; GO:0048263; P:determination of dorsal identity; IMP:BHF-UCL.
DR GO; GO:0030514; P:negative regulation of BMP signaling pathway; IDA:MGI.
DR GO; GO:0090027; P:negative regulation of monocyte chemotaxis; IDA:BHF-UCL.
DR GO; GO:0007399; P:nervous system development; IMP:BHF-UCL.
DR GO; GO:0030182; P:neuron differentiation; IDA:MGI.
DR GO; GO:0048812; P:neuron projection morphogenesis; IDA:MGI.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; IDA:MGI.
DR GO; GO:0038098; P:sequestering of BMP from receptor via BMP binding; IBA:GO_Central.
DR GO; GO:0035582; P:sequestering of BMP in extracellular matrix; IDA:BHF-UCL.
DR Gene3D; 2.10.90.10; -; 1.
DR InterPro; IPR006207; Cys_knot_C.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR004133; DAN.
DR InterPro; IPR016728; Neuroblast_suppress_tumour_1.
DR Pfam; PF03045; DAN; 1.
DR PIRSF; PIRSF018557; DAN_sub; 1.
DR SMART; SM00041; CT; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Reference proteome; Secreted; Signal; Tumor suppressor.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..178
FT /note="Neuroblastoma suppressor of tumorigenicity 1"
FT /id="PRO_0000006723"
FT DOMAIN 34..123
FT /note="CTCK"
FT REGION 132..178
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 34..84
FT /evidence="ECO:0000250"
FT DISULFID 48..98
FT /evidence="ECO:0000250"
FT DISULFID 58..117
FT /evidence="ECO:0000250"
FT DISULFID 62..119
FT /evidence="ECO:0000250"
FT DISULFID 81..122
FT /evidence="ECO:0000255"
FT CONFLICT 100
FT /note="G -> D (in Ref. 1; BAA08817)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 178 AA; 19107 MW; C51D74C82ED904DD CRC64;
MLWVLVGAVL PVMLLAAPPP INKLALFPDK SAWCEAKNIT QIVGHSGCEA KSIQNRACLG
QCFSYSVPNT FPQSTESLVH CDSCMPAQSM WEIVTLECPG HEEVPRVDKL VEKIVHCSCQ
ACGKEPSHEG LNVYVQGEDS PGSQPGPHSH AHPHPGGQTP EPEEPPGAPQ VEEEGAED
