NBL1_YEAST
ID NBL1_YEAST Reviewed; 73 AA.
AC Q3E7Y6; D3DLE8;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=N-terminal-borealin-like protein;
GN Name=NBL1; OrderedLocusNames=YHR199C-A;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP GENOME REANNOTATION.
RX PubMed=12775844; DOI=10.1126/science.1084337;
RA Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J.,
RA Waterston R., Cohen B.A., Johnston M.;
RT "Finding functional features in Saccharomyces genomes by phylogenetic
RT footprinting.";
RL Science 301:71-76(2003).
RN [4]
RP IDENTIFICATION IN THE AURORA KINASE COMPLEX, IDENTIFICATION BY MASS
RP SPECTROMETRY, FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=19158380; DOI=10.1091/mbc.e08-10-1011;
RA Nakajima Y., Tyers R.G., Wong C.C., Yates J.R. III, Drubin D.G., Barnes G.;
RT "Nbl1p: a Borealin/Dasra/CSC-1-like protein essential for Aurora/Ipl1
RT complex function and integrity in Saccharomyces cerevisiae.";
RL Mol. Biol. Cell 20:1772-1784(2009).
CC -!- FUNCTION: Component of the aurora kinase complex, also called
CC chromosomal passenger complex (CPC), essential for chromosome
CC segregation and metaphase chromosome alignment. Mediates the SLI15-BIR1
CC interaction within the CPC. {ECO:0000269|PubMed:19158380}.
CC -!- SUBUNIT: Component of the aurora kinase complex composed of at least
CC BIR1, BNL1, IPL1 and SLI15. {ECO:0000269|PubMed:19158380}.
CC -!- INTERACTION:
CC Q3E7Y6; P47134: BIR1; NbExp=5; IntAct=EBI-9513736, EBI-3648;
CC Q3E7Y6; P38283: SLI15; NbExp=5; IntAct=EBI-9513736, EBI-20842;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19158380}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000269|PubMed:19158380}. Note=In G1,
CC localizes along nuclear microtubules and at spindle poles. In late S
CC phase to metaphase, is seen as a few small nuclear foci and also
CC localizes diffusely within the nucleus. Also found along the spindle in
CC early anaphase, at the spindle midzone in late anaphase, and finally
CC concentrated at the plus ends of depolymerizing microtubules before
CC mitotic exit.
CC -!- SIMILARITY: Belongs to the borealin family. {ECO:0000305}.
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DR EMBL; U00030; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BK006934; DAA06892.1; -; Genomic_DNA.
DR RefSeq; NP_976247.1; NM_001184671.1.
DR AlphaFoldDB; Q3E7Y6; -.
DR SMR; Q3E7Y6; -.
DR BioGRID; 37086; 55.
DR ComplexPortal; CPX-1900; Chromosomal passenger complex.
DR IntAct; Q3E7Y6; 3.
DR STRING; 4932.YHR199C-A; -.
DR iPTMnet; Q3E7Y6; -.
DR MaxQB; Q3E7Y6; -.
DR PaxDb; Q3E7Y6; -.
DR PRIDE; Q3E7Y6; -.
DR EnsemblFungi; YHR199C-A_mRNA; YHR199C-A; YHR199C-A.
DR GeneID; 2746858; -.
DR KEGG; sce:YHR199C-A; -.
DR SGD; S000029704; NBL1.
DR VEuPathDB; FungiDB:YHR199C-A; -.
DR HOGENOM; CLU_2706256_0_0_1; -.
DR BioCyc; YEAST:G3O-31280-MON; -.
DR ChiTaRS; NBL1; yeast.
DR PRO; PR:Q3E7Y6; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; Q3E7Y6; protein.
DR GO; GO:0032133; C:chromosome passenger complex; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; IDA:SGD.
DR GO; GO:0051316; P:attachment of spindle microtubules to kinetochore involved in meiotic chromosome segregation; IC:ComplexPortal.
DR GO; GO:0007059; P:chromosome segregation; IMP:SGD.
DR GO; GO:0090267; P:positive regulation of mitotic cell cycle spindle assembly checkpoint; IC:ComplexPortal.
DR GO; GO:0031134; P:sister chromatid biorientation; IC:ComplexPortal.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Nucleus; Reference proteome.
FT CHAIN 1..73
FT /note="N-terminal-borealin-like protein"
FT /id="PRO_0000245397"
SQ SEQUENCE 73 AA; 8547 MW; 470C704C05F62FBC CRC64;
MIPALTPEER QKLRSAILHR MQLELETTEK LIENIKEETL KKLNLLQQPD ATSAPQSKEL
IREVLEQEGR RIE
