NBOR_PAENI
ID NBOR_PAENI Reviewed; 204 AA.
AC Q93NF6;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 58.
DE RecName: Full=Nicotine blue oxidoreductase;
DE EC=1.1.1.328;
GN Name=nboR;
OS Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans).
OG Plasmid pAO1.
OC Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Paenarthrobacter.
OX NCBI_TaxID=29320;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11514508; DOI=10.1128/jb.183.18.5262-5267.2001;
RA Baitsch D., Sandu C., Brandsch R., Igloi G.L.;
RT "Gene cluster on pAO1 of Arthrobacter nicotinovorans involved in
RT degradation of the plant alkaloid nicotine: cloning, purification, and
RT characterization of 2,6-dihydroxypyridine 3-hydroxylase.";
RL J. Bacteriol. 183:5262-5267(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12618462; DOI=10.1128/jb.185.6.1976-1986.2003;
RA Igloi G.L., Brandsch R.;
RT "Sequence of the 165-kilobase catabolic plasmid pAO1 from Arthrobacter
RT nicotinovorans and identification of a pAO1-dependent nicotine uptake
RT system.";
RL J. Bacteriol. 185:1976-1986(2003).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP SUBUNIT.
RX PubMed=17293530; DOI=10.1128/aem.02668-06;
RA Mihasan M., Chiribau C.B., Friedrich T., Artenie V., Brandsch R.;
RT "An NAD(P)H-nicotine blue oxidoreductase is part of the nicotine regulon
RT and may protect Arthrobacter nicotinovorans from oxidative stress during
RT nicotine catabolism.";
RL Appl. Environ. Microbiol. 73:2479-2485(2007).
CC -!- FUNCTION: Catalyzes the reduction of nicotine blue to its hydroquinone
CC form. Nicotine blue is the name given to the compound formed by the
CC autocatalytic condensation of two molecules of 2,3,6-
CC trihydroxypyridine, an intermediate in the nicotine degradation
CC pathway. May play a role in preventing the intracellular formation of
CC nicotine blue semiquinone radicals, which by redox cycling would lead
CC to the formation of toxic reactive oxygen species. Besides nicotine
CC blue, several other quinones are reduced by nboR.
CC {ECO:0000269|PubMed:17293530}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3,3'-bipyridine-2,2',5,5',6,6'-hexol + NADP(+) = (E)-
CC 2,2',5,5'-tetrahydroxy-6H,6'H-(3,3'-bipyridinylidene)-6,6'-dione + 3
CC H(+) + NADPH; Xref=Rhea:RHEA:34027, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:64998,
CC ChEBI:CHEBI:64999; EC=1.1.1.328;
CC Evidence={ECO:0000269|PubMed:17293530};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3,3'-bipyridine-2,2',5,5',6,6'-hexol + NAD(+) = (E)-2,2',5,5'-
CC tetrahydroxy-6H,6'H-(3,3'-bipyridinylidene)-6,6'-dione + 3 H(+) +
CC NADH; Xref=Rhea:RHEA:34023, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:64998, ChEBI:CHEBI:64999;
CC EC=1.1.1.328; Evidence={ECO:0000269|PubMed:17293530};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000269|PubMed:17293530};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=6.25 uM for 1,4-naphthoquinone {ECO:0000269|PubMed:17293530};
CC KM=8.83 uM for 1,4-benzoquinone {ECO:0000269|PubMed:17293530};
CC KM=23 uM for 2,6-dichlorophenolindophenol
CC {ECO:0000269|PubMed:17293530};
CC -!- PATHWAY: Alkaloid degradation; nicotine degradation.
CC -!- SUBUNIT: Homotetramer. {ECO:0000269|PubMed:17293530}.
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DR EMBL; AF373840; AAK64261.1; -; Genomic_DNA.
DR EMBL; AJ507836; CAD47930.1; -; Genomic_DNA.
DR RefSeq; WP_016359441.1; NC_021229.1.
DR RefSeq; YP_007988756.1; NC_021229.1.
DR AlphaFoldDB; Q93NF6; -.
DR SMR; Q93NF6; -.
DR KEGG; ag:CAD47930; -.
DR SABIO-RK; Q93NF6; -.
DR UniPathway; UPA00106; -.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0009820; P:alkaloid metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019608; P:nicotine catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR025877; MobA-like_NTP_Trfase.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF12804; NTP_transf_3; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 1: Evidence at protein level;
KW Alkaloid metabolism; NADP; Oxidoreductase; Plasmid.
FT CHAIN 1..204
FT /note="Nicotine blue oxidoreductase"
FT /id="PRO_0000424088"
SQ SEQUENCE 204 AA; 21537 MW; 893F0CE27D40CF6B CRC64;
MGDTDLPCVT GVLLAAGAGK RLGRGPKALL PYRGRTLVED AAETMLVGGC HEVVIVLGAN
AQAVCARANL EPYRIVVNHD WSSGMGSSYL AGDAAAHTKN HILVALVDQP GLSVTTVGRL
LVSHRPGRIS SAAYSSLDSP RVLRRGHPMV IDAGLRPAVA STVSGDAGAR VFLRQKPWLV
DLIDCSDEST GEDVDTVEQM YRLL
