NBS1_ARATH
ID NBS1_ARATH Reviewed; 542 AA.
AC Q0H8D7; A1YEB0; Q9M874;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Nibrin homolog {ECO:0000305};
DE AltName: Full=Nijmegen breakage syndrome 1 protein {ECO:0000303|PubMed:17182003};
DE Short=AtNbs1 {ECO:0000303|PubMed:17182003};
GN Name=NBS1 {ECO:0000303|PubMed:17182003};
GN OrderedLocusNames=At3g02680 {ECO:0000312|Araport:AT3G02680};
GN ORFNames=F16B3.31 {ECO:0000312|EMBL:AAF32475.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:ABA54896.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND INDUCTION BY X-RAYS.
RC STRAIN=cv. Columbia;
RX PubMed=17182003; DOI=10.1016/j.bbrc.2006.12.030;
RA Akutsu N., Iijima K., Hinata T., Tauchi H.;
RT "Characterization of the plant homolog of Nijmegen breakage syndrome 1:
RT Involvement in DNA repair and recombination.";
RL Biochem. Biophys. Res. Commun. 353:394-398(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DISRUPTION PHENOTYPE,
RP INTERACTION WITH MRE11, AND MUTAGENESIS OF 465-VAL--VAL-500.
RC STRAIN=cv. Columbia;
RX PubMed=17672843; DOI=10.1111/j.1365-313x.2007.03220.x;
RA Waterworth W.M., Altun C., Armstrong S.J., Roberts N., Dean P.J., Young K.,
RA Weil C.F., Bray C.M., West C.E.;
RT "NBS1 is involved in DNA repair and plays a synergistic role with ATM in
RT mediating meiotic homologous recombination in plants.";
RL Plant J. 52:41-52(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP REVIEW ON DNA REPAIR.
RX PubMed=16202663; DOI=10.1016/j.dnarep.2005.08.017;
RA Bleuyard J.Y., Gallego M.E., White C.I.;
RT "Recent advances in understanding of the DNA double-strand break repair
RT machinery of plants.";
RL DNA Repair 5:1-12(2006).
CC -!- FUNCTION: Component of the MRE11-RAD50-NBN complex (MRN complex) which
CC plays a critical role in the cellular response to DNA damage and the
CC maintenance of chromosome integrity. The complex may be involved in
CC double-strand break (DSB) repair, DNA recombination, maintenance of
CC telomere integrity, and cell cycle checkpoint control. Functions also
CC in the very early stages of meiosis. {ECO:0000269|PubMed:17672843}.
CC -!- SUBUNIT: Component of the MRN complex composed of two heterodimers
CC RAD50/MRE11 associated with a single NBN (By similarity). Interacts
CC with MRE11. {ECO:0000250|UniProtKB:O60934,
CC ECO:0000269|PubMed:17672843}.
CC -!- SUBCELLULAR LOCATION: Nucleus, PML body {ECO:0000250|UniProtKB:O60934}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0H8D7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0H8D7-2; Sequence=VSP_057110;
CC -!- INDUCTION: By X-rays. {ECO:0000269|PubMed:17182003}.
CC -!- DOMAIN: The FHA and BRCT domains are likely to have a crucial role for
CC both binding to histone H2AFX and for relocalization of MRE11/RAD50
CC complex to the vicinity of DNA damage. {ECO:0000250|UniProtKB:O60934}.
CC -!- DISRUPTION PHENOTYPE: Hypersensitivity to the DNA cross-linking reagent
CC mitomycin C. {ECO:0000269|PubMed:17672843}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF32475.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DQ917672; ABK59968.1; -; mRNA.
DR EMBL; DQ167217; ABA54896.1; -; mRNA.
DR EMBL; AC021640; AAF32475.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE73847.1; -; Genomic_DNA.
DR RefSeq; NP_186917.2; NM_111136.3. [Q0H8D7-1]
DR AlphaFoldDB; Q0H8D7; -.
DR STRING; 3702.AT3G02680.1; -.
DR iPTMnet; Q0H8D7; -.
DR PaxDb; Q0H8D7; -.
DR PRIDE; Q0H8D7; -.
DR ProteomicsDB; 251201; -. [Q0H8D7-1]
DR EnsemblPlants; AT3G02680.1; AT3G02680.1; AT3G02680. [Q0H8D7-1]
DR GeneID; 821254; -.
DR Gramene; AT3G02680.1; AT3G02680.1; AT3G02680. [Q0H8D7-1]
DR KEGG; ath:AT3G02680; -.
DR Araport; AT3G02680; -.
DR TAIR; locus:2076934; AT3G02680.
DR eggNOG; ENOG502QQ7Y; Eukaryota.
DR HOGENOM; CLU_036857_0_0_1; -.
DR InParanoid; Q0H8D7; -.
DR OMA; DEFCANS; -.
DR OrthoDB; 831679at2759; -.
DR PhylomeDB; Q0H8D7; -.
DR PRO; PR:Q0H8D7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q0H8D7; baseline and differential.
DR Genevisible; Q0H8D7; AT.
DR GO; GO:0030870; C:Mre11 complex; IBA:GO_Central.
DR GO; GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
DR GO; GO:0003684; F:damaged DNA binding; IBA:GO_Central.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IMP:UniProtKB.
DR GO; GO:0071479; P:cellular response to ionizing radiation; IEP:UniProtKB.
DR GO; GO:0032508; P:DNA duplex unwinding; IBA:GO_Central.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IMP:UniProtKB.
DR GO; GO:0007095; P:mitotic G2 DNA damage checkpoint signaling; IBA:GO_Central.
DR GO; GO:0006312; P:mitotic recombination; IMP:UniProtKB.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:UniProtKB.
DR CDD; cd00060; FHA; 1.
DR Gene3D; 3.40.50.10190; -; 1.
DR InterPro; IPR036420; BRCT_dom_sf.
DR InterPro; IPR000253; FHA_dom.
DR InterPro; IPR040227; Nibrin-rel.
DR InterPro; IPR008984; SMAD_FHA_dom_sf.
DR PANTHER; PTHR12162; PTHR12162; 1.
DR Pfam; PF00498; FHA; 1.
DR SUPFAM; SSF49879; SSF49879; 1.
DR PROSITE; PS50006; FHA_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; DNA damage; DNA repair; Nucleus;
KW Reference proteome.
FT CHAIN 1..542
FT /note="Nibrin homolog"
FT /id="PRO_0000430944"
FT DOMAIN 25..90
FT /note="FHA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00086"
FT DOMAIN 119..195
FT /note="BRCT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00033"
FT REGION 409..430
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 465..500
FT /note="Involved in MRE11-binding"
FT /evidence="ECO:0000269|PubMed:17672843"
FT VAR_SEQ 334..342
FT /note="Missing (in isoform 2)"
FT /id="VSP_057110"
FT MUTAGEN 465..500
FT /note="Missing: Impaired MRE11-binding."
FT /evidence="ECO:0000269|PubMed:17672843"
SQ SEQUENCE 542 AA; 60078 MW; 0CAD7920E4682628 CRC64;
MVWGLFPVDP LSGEDKYYIF SKGIYKVGRK GCDIIINKDK GVSRIHAELT FDATTVSTSR
RNKSSSDTSS FVIRVKDCSK YGTFVKTDLG TKDKVHELSN KEKILQDGDV IAFGTGSAIY
RLSLIPLVFY LCPSSETFKV DQPVQDAVSS IGARISPTLS EECTHVLLEP RMQVNEALIN
AILAKKTIIL TNWVMLLAEK SICSEIPGYS QYRPSVMVEE ALVDVLELNV REKCLEGFTF
VLEPTDTYRF GCSFPSLLEV CGAETVTIED ISSMSQDSQF GEINRMICVI PKSAGDKFGR
FKHLSLLSRV NEMDLVCAVF SGNLPSTSLI PPSVVISSSC STDETVVADS EAEEEETTSS
VHMIDATEKA ETPEKPAAIV IEDSPVTILE ETSNLNEFKS VNLLADTESR GHMDEKNSSD
SVTIRRDRND EAETGKSEII YTQDLIVRDL RSTRKVQSTG GEGVVDFKRF RKGNVTCGNS
FSSLIPFAKD PYKEYDSWDV TDFMKEEKKR KQMEAIAEDL FKTEKARKRG TAGSIRGFLS
GS
