NBS1_ORYSI
ID NBS1_ORYSI Reviewed; 560 AA.
AC B8BHK8; B7UCQ8;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 60.
DE RecName: Full=Nibrin homolog {ECO:0000305};
DE AltName: Full=Nijmegen breakage syndrome 1 protein;
DE Short=OsNbs1;
GN Name=NBS1 {ECO:0000305}; ORFNames=OsI_34099 {ECO:0000312|EMBL:EEC67210.1};
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946 {ECO:0000312|EMBL:EEC67210.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-536.
RA Rajanikant C., Sainis J.K.;
RT "Characterization of NBS1-like protein from Oryza sativa.";
RL Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the MRE11-RAD50-NBN complex (MRN complex) which
CC plays a critical role in the cellular response to DNA damage and the
CC maintenance of chromosome integrity. The complex may be involved in
CC double-strand break (DSB) repair, DNA recombination, maintenance of
CC telomere integrity, and cell cycle checkpoint control. Functions also
CC in the very early stages of meiosis. {ECO:0000250|UniProtKB:Q0H8D7}.
CC -!- SUBUNIT: Component of the MRN complex composed of two heterodimers
CC RAD50/MRE11 associated with a single NBN (By similarity). Interacts
CC with MRE11 (By similarity). {ECO:0000250|UniProtKB:O60934,
CC ECO:0000250|UniProtKB:Q7XD82}.
CC -!- SUBCELLULAR LOCATION: Nucleus, PML body {ECO:0000250|UniProtKB:O60934}.
CC -!- DOMAIN: The FHA and BRCT domains are likely to have a crucial role for
CC both binding to histone H2AFX and for relocalization of MRE11/RAD50
CC complex to the vicinity of DNA damage. {ECO:0000250|UniProtKB:O60934}.
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DR EMBL; CM000135; EEC67210.1; -; Genomic_DNA.
DR EMBL; FJ475131; ACK43220.1; -; mRNA.
DR AlphaFoldDB; B8BHK8; -.
DR STRING; 39946.B8BHK8; -.
DR PRIDE; B8BHK8; -.
DR EnsemblPlants; BGIOSGA033164-TA; BGIOSGA033164-PA; BGIOSGA033164.
DR Gramene; BGIOSGA033164-TA; BGIOSGA033164-PA; BGIOSGA033164.
DR HOGENOM; CLU_036857_0_0_1; -.
DR OMA; DEFCANS; -.
DR Proteomes; UP000007015; Chromosome 10.
DR GO; GO:0030870; C:Mre11 complex; IEA:InterPro.
DR GO; GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; ISS:UniProtKB.
DR GO; GO:0071479; P:cellular response to ionizing radiation; ISS:UniProtKB.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; ISS:UniProtKB.
DR GO; GO:0007095; P:mitotic G2 DNA damage checkpoint signaling; IEA:InterPro.
DR GO; GO:0006312; P:mitotic recombination; ISS:UniProtKB.
DR GO; GO:0007131; P:reciprocal meiotic recombination; ISS:UniProtKB.
DR CDD; cd00060; FHA; 1.
DR Gene3D; 3.40.50.10190; -; 1.
DR InterPro; IPR036420; BRCT_dom_sf.
DR InterPro; IPR000253; FHA_dom.
DR InterPro; IPR040227; Nibrin-rel.
DR InterPro; IPR008984; SMAD_FHA_dom_sf.
DR PANTHER; PTHR12162; PTHR12162; 1.
DR Pfam; PF00498; FHA; 1.
DR SUPFAM; SSF49879; SSF49879; 1.
DR SUPFAM; SSF52113; SSF52113; 1.
DR PROSITE; PS50006; FHA_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; DNA damage; DNA repair; Nucleus; Reference proteome.
FT CHAIN 1..560
FT /note="Nibrin homolog"
FT /id="PRO_0000430946"
FT DOMAIN 25..87
FT /note="FHA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00086"
FT DOMAIN 115..190
FT /note="BRCT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00033"
FT MOTIF 511..518
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT CONFLICT 113
FT /note="S -> N (in Ref. 2; ACK43220)"
FT CONFLICT 138
FT /note="S -> C (in Ref. 2; ACK43220)"
FT CONFLICT 200
FT /note="A -> E (in Ref. 2; ACK43220)"
FT CONFLICT 297
FT /note="S -> R (in Ref. 2; ACK43220)"
FT CONFLICT 400
FT /note="Y -> D (in Ref. 2; ACK43220)"
FT CONFLICT 512
FT /note="K -> E (in Ref. 2; ACK43220)"
FT CONFLICT 517
FT /note="I -> T (in Ref. 2; ACK43220)"
SQ SEQUENCE 560 AA; 61883 MW; F59A50406B8FCC55 CRC64;
MVWALTPVDT VRGAQRCYIF AAGTYKVGRK DCDVIVQTDT SISRVHAEIV VEKMVAWDPQ
SGAPANPSYV RVVDRSKYGT FFNKVQGTQG SRLHKDEDAM LADGDTVTFG TGSATFRLSF
VPIVVFFHGK KSGRISPSLQ AVMTSIGAYA TRKWSDECTH VLVDESCSLT PELLDAVLAK
KQIVLGDWFK VMAEKNIHTA MPSSTQYIPK LTLDGMEIQV VEIKLIESCL AGYTFILGSS
EKYKFGDKLH ALLESTGAKY LHVDEFCANS QDSGAGENDK DILLVPAKSP LEFSKISGLF
PLSKITDVKL FAAILSGHLE ATAIEPPAYI VASSNSTDET IVVDSDVEID TATSDHTVAA
SKSEHHIEHI SDDKKEVVAI SEEDAVNLVE AKTSINLHSY QEKDEIVKPM EEDVKVIEKT
ATMRGFKVEG EDIPVMTKVP KDETLDSRDE TCHVIYTQNL VVKSILQSAR AESIETGGIN
FKRFRKRGAV SGNSFKDLIP YSREPYRESD YKRGTVIDFM REEKKRRQME AIAEDLFNNA
KPKKKAAAGS SIHTMLTGRR
