NBS1_ORYSJ
ID NBS1_ORYSJ Reviewed; 560 AA.
AC Q7XD82; A0A0P0XVJ9; Q8W382;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Nibrin homolog {ECO:0000305};
DE AltName: Full=Nijmegen breakage syndrome 1 protein {ECO:0000303|PubMed:17182003};
DE Short=OsNbs1 {ECO:0000303|PubMed:17182003};
GN Name=NBS1 {ECO:0000303|PubMed:17182003};
GN OrderedLocusNames=LOC_Os10g34580 {ECO:0000312|EMBL:AAP54361.1},
GN Os10g0487300 {ECO:0000312|EMBL:BAF26819.1};
GN ORFNames=OsJ_31956 {ECO:0000312|EMBL:EEE51174.1},
GN OSJNBa0029C15.11 {ECO:0000312|EMBL:AAL59021.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947 {ECO:0000312|Proteomes:UP000059680};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12791992; DOI=10.1126/science.1083523;
RA Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S.,
RA Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D.,
RA Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M.,
RA Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F.,
RA Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M.,
RA Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R.,
RA Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F.,
RA Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D.,
RA Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R.,
RA Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S.,
RA Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S.,
RA Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R.,
RA Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M.,
RA Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E.,
RA Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT "In-depth view of structure, activity, and evolution of rice chromosome
RT 10.";
RL Science 300:1566-1569(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP INTERACTION WITH MRE11, AND TISSUE SPECIFICITY.
RX PubMed=17182003; DOI=10.1016/j.bbrc.2006.12.030;
RA Akutsu N., Iijima K., Hinata T., Tauchi H.;
RT "Characterization of the plant homolog of Nijmegen breakage syndrome 1:
RT Involvement in DNA repair and recombination.";
RL Biochem. Biophys. Res. Commun. 353:394-398(2007).
CC -!- FUNCTION: Component of the MRE11-RAD50-NBN complex (MRN complex) which
CC plays a critical role in the cellular response to DNA damage and the
CC maintenance of chromosome integrity. The complex may be involved in
CC double-strand break (DSB) repair, DNA recombination, maintenance of
CC telomere integrity, and cell cycle checkpoint control. Functions also
CC in the very early stages of meiosis. {ECO:0000250|UniProtKB:Q0H8D7}.
CC -!- SUBUNIT: Component of the MRN complex composed of two heterodimers
CC RAD50/MRE11 associated with a single NBN (By similarity). Interacts
CC with MRE11. {ECO:0000250|UniProtKB:O60934,
CC ECO:0000269|PubMed:17182003}.
CC -!- SUBCELLULAR LOCATION: Nucleus, PML body {ECO:0000250|UniProtKB:O60934}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in the shoot apex and young
CC flower, but also in young leaves, root tips and stamen, tissues where
CC frequent cell division or meiosis may occur.
CC {ECO:0000269|PubMed:17182003}.
CC -!- DOMAIN: The FHA and BRCT domains are likely to have a crucial role for
CC both binding to histone H2AFX and for relocalization of MRE11/RAD50
CC complex to the vicinity of DNA damage. {ECO:0000250|UniProtKB:O60934}.
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DR EMBL; AC087182; AAL59021.1; -; Genomic_DNA.
DR EMBL; DP000086; AAP54361.1; -; Genomic_DNA.
DR EMBL; AP008216; BAF26819.1; -; Genomic_DNA.
DR EMBL; AP014966; BAT11396.1; -; Genomic_DNA.
DR EMBL; CM000147; EEE51174.1; -; Genomic_DNA.
DR EMBL; AK069561; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015614711.1; XM_015759225.1.
DR AlphaFoldDB; Q7XD82; -.
DR STRING; 4530.OS10T0487300-01; -.
DR iPTMnet; Q7XD82; -.
DR PaxDb; Q7XD82; -.
DR PRIDE; Q7XD82; -.
DR EnsemblPlants; Os10t0487300-01; Os10t0487300-01; Os10g0487300.
DR GeneID; 4348951; -.
DR Gramene; Os10t0487300-01; Os10t0487300-01; Os10g0487300.
DR KEGG; osa:4348951; -.
DR eggNOG; ENOG502QQ7Y; Eukaryota.
DR HOGENOM; CLU_036857_0_0_1; -.
DR InParanoid; Q7XD82; -.
DR OMA; DEFCANS; -.
DR OrthoDB; 831679at2759; -.
DR Proteomes; UP000000763; Chromosome 10.
DR Proteomes; UP000007752; Chromosome 10.
DR Proteomes; UP000059680; Chromosome 10.
DR Genevisible; Q7XD82; OS.
DR GO; GO:0030870; C:Mre11 complex; IBA:GO_Central.
DR GO; GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
DR GO; GO:0003684; F:damaged DNA binding; IBA:GO_Central.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; ISS:UniProtKB.
DR GO; GO:0071479; P:cellular response to ionizing radiation; ISS:UniProtKB.
DR GO; GO:0032508; P:DNA duplex unwinding; IBA:GO_Central.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; ISS:UniProtKB.
DR GO; GO:0007095; P:mitotic G2 DNA damage checkpoint signaling; IBA:GO_Central.
DR GO; GO:0006312; P:mitotic recombination; ISS:UniProtKB.
DR GO; GO:0007131; P:reciprocal meiotic recombination; ISS:UniProtKB.
DR CDD; cd00060; FHA; 1.
DR Gene3D; 3.40.50.10190; -; 1.
DR InterPro; IPR036420; BRCT_dom_sf.
DR InterPro; IPR013908; DNA-repair_Nbs1_C.
DR InterPro; IPR000253; FHA_dom.
DR InterPro; IPR040227; Nibrin-rel.
DR InterPro; IPR008984; SMAD_FHA_dom_sf.
DR PANTHER; PTHR12162; PTHR12162; 1.
DR Pfam; PF00498; FHA; 1.
DR SMART; SM01348; Nbs1_C; 1.
DR SUPFAM; SSF49879; SSF49879; 1.
DR SUPFAM; SSF52113; SSF52113; 1.
DR PROSITE; PS50006; FHA_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Cell cycle; DNA damage; DNA repair; Nucleus; Reference proteome.
FT CHAIN 1..560
FT /note="Nibrin homolog"
FT /id="PRO_0000430945"
FT DOMAIN 25..87
FT /note="FHA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00086"
FT DOMAIN 115..190
FT /note="BRCT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00033"
FT CONFLICT 463
FT /note="K -> E (in Ref. 5; AK069561)"
SQ SEQUENCE 560 AA; 61994 MW; A7CED9A1542DB290 CRC64;
MVWALTPVDT VRGAQRCYIF AAGTYKVGRK DCDVIVQTDT SISRVHAEIV VEKMVAWDPQ
SGAPANPSYV RVVDRSKYGT FFNKVQGTQG SRLHKDEDAM LADGDTVTFG TGNATFRLSF
VPIVVFFHGK KSGRISPCLQ AVMTSIGAYA TRKWSDECTH VLVDESCSLT PELLDAVLAK
KQIVLGDWFK VMAEKNIHTE MPSSTQYIPK LTLDGMEIQV VEIKLIESCL AGYTFILGSS
EKYKFGDKLH ALLESTGAKY LHVDEFCANS QDSGAGENDK DILLVPAKSP LEFSKIRGLF
PLSKITDVKL FAAILSGHLE ATAIEPPAYI VASSNSTDET IVVDSDVEID TATSDHTVAA
SKSEHHIEHI SDDKKEVVAI SEEDAVNLVE AKTSINLHSD QEKDEIVKPM EEDVKVIEKT
ATMRGFKVEG EDIPVMTKVP KDETLDSRDE TCHVIYTQNL VVKSILQSAR AESIETGGIN
FKRFRKRGAV SGNSFKDLIP YSREPYRESD YERGTVTDFM REEKKRRQME AIAEDLFNNA
KPKKKAAAGS SIHTMLTGRR
