NBS_NARPS
ID NBS_NARPS Reviewed; 163 AA.
AC A0A3G5BB24;
DT 12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT 13-FEB-2019, sequence version 1.
DT 03-AUG-2022, entry version 9.
DE RecName: Full=Norbelladine synthase {ECO:0000303|PubMed:30526483};
DE Short=NpNBS {ECO:0000303|PubMed:30526483};
DE EC=4.2.1.- {ECO:0000269|PubMed:30526483};
GN Name=NBS {ECO:0000303|PubMed:30526483};
OS Narcissus pseudonarcissus (Daffodil).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Amaryllidaceae;
OC Amaryllidoideae; Narcissus.
OX NCBI_TaxID=39639;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY, TISSUE
RP SPECIFICITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC STRAIN=cv. King Alfred; TISSUE=Bulb;
RX PubMed=30526483; DOI=10.1186/s12870-018-1570-4;
RA Singh A., Massicotte M.-A., Garand A., Tousignant L., Ouellette V.,
RA Berube G., Desgagne-Penix I.;
RT "Cloning and characterization of norbelladine synthase catalyzing the first
RT committed reaction in Amaryllidaceae alkaloid biosynthesis.";
RL BMC Plant Biol. 18:338-338(2018).
RN [2]
RP REVIEW ON THE AMARYLLIDACEAE ALKALOID METABOLISM.
RC STRAIN=cv. King Alfred; TISSUE=Bulb;
RX PubMed=29229969; DOI=10.1038/s41598-017-17724-0;
RA Singh A., Desgagne-Penix I.;
RT "Transcriptome and metabolome profiling of Narcissus pseudonarcissus 'King
RT Alfred' reveal components of Amaryllidaceae alkaloid metabolism.";
RL Sci. Rep. 7:17356-17356(2017).
CC -!- FUNCTION: Catalyzes the condensation of tyramine and 3,4-
CC dihydroxybenzaldehyde (3,4-DHBA) to form norbelladine, the common
CC precursor to all Amaryllidaceae alkaloids such as galanthamine,
CC lycorine and haemanthamine, and including haemanthamine- and crinamine-
CC type alkaloids, promising anticancer agents.
CC {ECO:0000269|PubMed:30526483}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3,4-dihydroxybenzaldehyde + AH2 + tyramine = A + H2O +
CC norbelladine; Xref=Rhea:RHEA:63188, ChEBI:CHEBI:13193,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:17499, ChEBI:CHEBI:50205,
CC ChEBI:CHEBI:134001, ChEBI:CHEBI:327995;
CC Evidence={ECO:0000269|PubMed:30526483};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63189;
CC Evidence={ECO:0000269|PubMed:30526483};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 4. {ECO:0000269|PubMed:30526483};
CC -!- PATHWAY: Alkaloid biosynthesis. {ECO:0000269|PubMed:30526483}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in bulbs, and, to a lower extent,
CC in roots, stems, leaves and flowers. {ECO:0000269|PubMed:30526483}.
CC -!- SIMILARITY: Belongs to the BetVI family. {ECO:0000305}.
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DR EMBL; MG948545; AYV96792.1; -; mRNA.
DR AlphaFoldDB; A0A3G5BB24; -.
DR SMR; A0A3G5BB24; -.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0009820; P:alkaloid metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:InterPro.
DR Gene3D; 3.30.530.20; -; 1.
DR InterPro; IPR000916; Bet_v_I/MLP.
DR InterPro; IPR023393; START-like_dom_sf.
DR Pfam; PF00407; Bet_v_1; 1.
DR SMART; SM01037; Bet_v_1; 1.
PE 1: Evidence at protein level;
KW Alkaloid metabolism; Lyase.
FT CHAIN 1..163
FT /note="Norbelladine synthase"
FT /id="PRO_0000450640"
FT ACT_SITE 83
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q67A25"
FT BINDING 68..71
FT /ligand="tyramine"
FT /ligand_id="ChEBI:CHEBI:327995"
FT /evidence="ECO:0000250|UniProtKB:Q67A25"
SQ SEQUENCE 163 AA; 18040 MW; F9D71B6F0466B59F CRC64;
MKGSLSHELE VSLPADQLWQ VYSTLRLAQL SAELLPTVIS KVEVEEGDGG VGTLLRVTYA
LGIPGMKYHK ERFVKIDHEK RLKEALFVEG GHLDLGFSSY LIRLEILEKG HNSSVIKSTV
EYEVDEEHAA NASFATTDPF MIIGGAVSEH LLQKKSNCSI MLL
