NC101_ARATH
ID NC101_ARATH Reviewed; 348 AA.
AC Q9LVA1;
DT 27-MAY-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=NAC domain-containing protein 101 {ECO:0000303|PubMed:15029955};
DE Short=ANAC101 {ECO:0000303|PubMed:15029955};
DE AltName: Full=Protein VASCULAR RELATED NAC-DOMAIN 6 {ECO:0000303|PubMed:16103214};
GN Name=NAC101 {ECO:0000303|PubMed:15029955};
GN Synonyms=VND6 {ECO:0000303|PubMed:16103214};
GN OrderedLocusNames=At5g62380 {ECO:0000312|Araport:AT5G62380};
GN ORFNames=MMI9.21 {ECO:0000312|EMBL:BAA97202.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15029955; DOI=10.1093/dnares/10.6.239;
RA Ooka H., Satoh K., Doi K., Nagata T., Otomo Y., Murakami K., Matsubara K.,
RA Osato N., Kawai J., Carninci P., Hayashizaki Y., Suzuki K., Kojima K.,
RA Takahara Y., Yamamoto K., Kikuchi S.;
RT "Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis
RT thaliana.";
RL DNA Res. 10:239-247(2003).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY,
RP SUBCELLULAR LOCATION, INDUCTION BY BRASSINOSTEROIDS; AUXIN AND CYTOKININ,
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16103214; DOI=10.1101/gad.1331305;
RA Kubo M., Udagawa M., Nishikubo N., Horiguchi G., Yamaguchi M., Ito J.,
RA Mimura T., Fukuda H., Demura T.;
RT "Transcription switches for protoxylem and metaxylem vessel formation.";
RL Genes Dev. 19:1855-1860(2005).
RN [7]
RP FUNCTION.
RX PubMed=17890373; DOI=10.1105/tpc.107.053678;
RA Zhong R., Richardson E.A., Ye Z.-H.;
RT "The MYB46 transcription factor is a direct target of SND1 and regulates
RT secondary wall biosynthesis in Arabidopsis.";
RL Plant Cell 19:2776-2792(2007).
RN [8]
RP TISSUE SPECIFICITY, AND HOMODIMER.
RC STRAIN=cv. Columbia;
RX PubMed=18445131; DOI=10.1111/j.1365-313x.2008.03533.x;
RA Yamaguchi M., Kubo M., Fukuda H., Demura T.;
RT "Vascular-related NAC-DOMAIN7 is involved in the differentiation of all
RT types of xylem vessels in Arabidopsis roots and shoots.";
RL Plant J. 55:652-664(2008).
RN [9]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=18952777; DOI=10.1105/tpc.108.061325;
RA Zhong R., Lee C., Zhou J., McCarthy R.L., Ye Z.H.;
RT "A battery of transcription factors involved in the regulation of secondary
RT cell wall biosynthesis in Arabidopsis.";
RL Plant Cell 20:2763-2782(2008).
RN [10]
RP FUNCTION.
RX PubMed=19088331; DOI=10.1105/tpc.108.061796;
RA Soyano T., Thitamadee S., Machida Y., Chua N.-H.;
RT "ASYMMETRIC LEAVES2-LIKE19/LATERAL ORGAN BOUNDARIES DOMAIN30 and
RT ASL20/LBD18 regulate tracheary element differentiation in Arabidopsis.";
RL Plant Cell 20:3359-3373(2008).
RN [11]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=19122102; DOI=10.1105/tpc.108.063321;
RA Zhou J., Lee C., Zhong R., Ye Z.-H.;
RT "MYB58 and MYB63 are transcriptional activators of the lignin biosynthetic
RT pathway during secondary cell wall formation in Arabidopsis.";
RL Plant Cell 21:248-266(2009).
RN [12]
RP FUNCTION.
RX PubMed=19808805; DOI=10.1093/pcp/pcp139;
RA McCarthy R.L., Zhong R., Ye Z.-H.;
RT "MYB83 is a direct target of SND1 and acts redundantly with MYB46 in the
RT regulation of secondary cell wall biosynthesis in Arabidopsis.";
RL Plant Cell Physiol. 50:1950-1964(2009).
RN [13]
RP FUNCTION.
RX PubMed=20935069; DOI=10.1093/mp/ssq062;
RA Zhong R., Lee C., Ye Z.-H.;
RT "Global analysis of direct targets of secondary wall NAC master switches in
RT Arabidopsis.";
RL Mol. Plant 3:1087-1103(2010).
RN [14]
RP FUNCTION.
RX PubMed=20952636; DOI=10.1105/tpc.110.075036;
RA Ohashi-Ito K., Oda Y., Fukuda H.;
RT "Arabidopsis VASCULAR-RELATED NAC-DOMAIN6 directly regulates the genes that
RT govern programmed cell death and secondary wall formation during xylem
RT differentiation.";
RL Plant Cell 22:3461-3473(2010).
RN [15]
RP FUNCTION.
RX PubMed=20488898; DOI=10.1104/pp.110.154013;
RA Yamaguchi M., Goue N., Igarashi H., Ohtani M., Nakano Y., Mortimer J.C.,
RA Nishikubo N., Kubo M., Katayama Y., Kakegawa K., Dupree P., Demura T.;
RT "VASCULAR-RELATED NAC-DOMAIN6 and VASCULAR-RELATED NAC-DOMAIN7 effectively
RT induce transdifferentiation into xylem vessel elements under control of an
RT induction system.";
RL Plant Physiol. 153:906-914(2010).
RN [16]
RP REVIEW.
RX PubMed=21847026; DOI=10.4161/psb.6.9.16402;
RA McCarthy R.L., Zhong R., Ye Z.-H.;
RT "Secondary wall NAC binding element (SNBE), a key cis-acting element
RT required for target gene activation by secondary wall NAC master
RT switches.";
RL Plant Signal. Behav. 6:1282-1285(2011).
RN [17]
RP INDUCTION BY VERTICILLIUM LONGISPORUM.
RC STRAIN=cv. Columbia;
RX PubMed=23023171; DOI=10.1105/tpc.112.103374;
RA Reusche M., Thole K., Janz D., Truskina J., Rindfleisch S., Drubert C.,
RA Polle A., Lipka V., Teichmann T.;
RT "Verticillium infection triggers VASCULAR-RELATED NAC DOMAIN7-dependent de
RT novo xylem formation and enhances drought tolerance in Arabidopsis.";
RL Plant Cell 24:3823-3837(2012).
CC -!- FUNCTION: Transcription activator that binds to the secondary wall NAC
CC binding element (SNBE), 5'-
CC (T/A)NN(C/T)(T/C/G)TNNNNNNNA(A/C)GN(A/C/T)(A/T)-3', and to the
CC tracheary elements (TE) specific regulating cis-element (TERE), 5'-
CC CTTNAAAGCNA-3', in the promoter of target genes (e.g. genes involved in
CC secondary wall biosynthesis, cell wall modification such as xylan
CC accumulation, and programmed cell death) (PubMed:20935069,
CC PubMed:20952636, PubMed:20488898). Involved in xylem formation in roots
CC and shoots, especially regulating metaxylem vessel differentiation by
CC promoting immature xylem vessel-specific genes expression, especially
CC genes regulating programmed cell death (PCD) and secondary wall
CC formation in tracheary elements (TE) (PubMed:16103214, PubMed:20952636,
CC PubMed:20488898). Can activate MYB25, MYB46, MYB58, MYB63, MYB83,
CC MYB103, CESA4, LBD15, LBD30, ERF115, XCP1, XCP2, NAC010/SND3, KNAT7,
CC ASL19 and ASL20 expression (PubMed:17890373, PubMed:18952777,
CC PubMed:19088331, PubMed:19122102, PubMed:19808805, PubMed:20935069,
CC PubMed:20952636). {ECO:0000269|PubMed:16103214,
CC ECO:0000269|PubMed:17890373, ECO:0000269|PubMed:18952777,
CC ECO:0000269|PubMed:19088331, ECO:0000269|PubMed:19122102,
CC ECO:0000269|PubMed:19808805, ECO:0000269|PubMed:20488898,
CC ECO:0000269|PubMed:20935069, ECO:0000269|PubMed:20952636}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:18445131}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00353,
CC ECO:0000269|PubMed:16103214}.
CC -!- TISSUE SPECIFICITY: Expressed in root inner metaxylem vessels and in
CC hypocotyl vessels (PubMed:18445131, PubMed:18952777). Present in root
CC developing xylems (PubMed:16103214). Accumulates in the xylem but not
CC in interfascicular fibers or pith cells in inflorescence stems. Absent
CC from secondary xylem in roots (PubMed:18952777).
CC {ECO:0000269|PubMed:16103214, ECO:0000269|PubMed:18445131,
CC ECO:0000269|PubMed:18952777}.
CC -!- DEVELOPMENTAL STAGE: Up-regulated during xylem vessel element
CC formation. Expressed preferentially in procambial cells adjacent to
CC root meristem, especially newly proliferating cells derived presumably
CC from pericycle. {ECO:0000269|PubMed:16103214}.
CC -!- INDUCTION: By brassinosteroids (e.g. brassinolide BL), auxin (e.g. 2,4-
CC dichlorphenoxyacetic acid 2,4-D) and cytokinin (e.g. kinetin), with a
CC synergistic effect (PubMed:16103214). Accumulates during infection by
CC the soilborne fungal pathogen Verticillium longisporum, especially in
CC tissues undergoing de novo xylem formation (PubMed:23023171).
CC {ECO:0000269|PubMed:16103214, ECO:0000269|PubMed:23023171}.
CC -!- DOMAIN: The NAC domain includes a DNA binding domain and a dimerization
CC domain. {ECO:0000255|PROSITE-ProRule:PRU00353}.
CC -!- DISRUPTION PHENOTYPE: Defects in metaxylem vessel formation in seedling
CC roots. {ECO:0000269|PubMed:16103214}.
CC -!- SIMILARITY: Belongs to the plant vascular related NAC-domain protein
CC family. {ECO:0000305}.
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DR EMBL; AB019235; BAA97202.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97602.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69996.1; -; Genomic_DNA.
DR EMBL; DQ056734; AAY78878.1; -; mRNA.
DR EMBL; BT026510; ABH04617.1; -; mRNA.
DR RefSeq; NP_001318864.1; NM_001345546.1.
DR RefSeq; NP_201044.1; NM_125632.2.
DR AlphaFoldDB; Q9LVA1; -.
DR SMR; Q9LVA1; -.
DR IntAct; Q9LVA1; 1.
DR STRING; 3702.AT5G62380.1; -.
DR PaxDb; Q9LVA1; -.
DR PRIDE; Q9LVA1; -.
DR ProteomicsDB; 236813; -.
DR EnsemblPlants; AT5G62380.1; AT5G62380.1; AT5G62380.
DR EnsemblPlants; AT5G62380.2; AT5G62380.2; AT5G62380.
DR GeneID; 836359; -.
DR Gramene; AT5G62380.1; AT5G62380.1; AT5G62380.
DR Gramene; AT5G62380.2; AT5G62380.2; AT5G62380.
DR KEGG; ath:AT5G62380; -.
DR Araport; AT5G62380; -.
DR TAIR; locus:2167923; AT5G62380.
DR eggNOG; ENOG502QT6P; Eukaryota.
DR HOGENOM; CLU_035664_1_0_1; -.
DR InParanoid; Q9LVA1; -.
DR OMA; ESNWFTH; -.
DR OrthoDB; 688436at2759; -.
DR PhylomeDB; Q9LVA1; -.
DR PRO; PR:Q9LVA1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LVA1; baseline and differential.
DR Genevisible; Q9LVA1; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0009531; C:secondary cell wall; IMP:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0090058; P:metaxylem development; IMP:UniProtKB.
DR GO; GO:0043068; P:positive regulation of programmed cell death; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0010981; P:regulation of cell wall macromolecule metabolic process; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0009737; P:response to abscisic acid; IEP:TAIR.
DR GO; GO:0009733; P:response to auxin; IEP:UniProtKB.
DR GO; GO:0009741; P:response to brassinosteroid; IEP:TAIR.
DR GO; GO:0009735; P:response to cytokinin; IEP:TAIR.
DR GO; GO:0009620; P:response to fungus; IDA:UniProtKB.
DR GO; GO:0045491; P:xylan metabolic process; IMP:UniProtKB.
DR GO; GO:0010089; P:xylem development; IMP:TAIR.
DR GO; GO:0048759; P:xylem vessel member cell differentiation; IMP:UniProtKB.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 2: Evidence at transcript level;
KW Activator; Cell wall biogenesis/degradation; Developmental protein;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..348
FT /note="NAC domain-containing protein 101"
FT /id="PRO_0000433123"
FT DOMAIN 7..156
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 107..162
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT REGION 325..348
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 348 AA; 39730 MW; F3AD473476451795 CRC64;
MESLAHIPPG YRFHPTDEEL VDYYLKNKVA FPGMQVDVIK DVDLYKIEPW DIQELCGRGT
GEEREWYFFS HKDKKYPTGT RTNRATGSGF WKATGRDKAI YSKQELVGMR KTLVFYKGRA
PNGQKSDWIM HEYRLETDEN GPPHEEGWVV CRAFKKKLTT MNYNNPRTMM GSSSGQESNW
FTQQMDVGNG NYYHLPDLES PRMFQGSSSS SLSSLHQNDQ DPYGVVLSTI NATPTTIMQR
DDGHVITNDD DHMIMMNTST GDHHQSGLLV NDDHNDQVMD WQTLDKFVAS QLIMSQEEEE
VNKDPSDNSS NETFHHLSEE QAATMVSMNA SSSSSPCSFY SWAQNTHT
