NC2A_DICDI
ID NC2A_DICDI Reviewed; 550 AA.
AC Q54DA1;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=Dr1-associated corepressor homolog;
DE AltName: Full=Negative cofactor 2-alpha homolog;
DE Short=NC2-alpha homolog;
GN Name=drap1; ORFNames=DDB_G0292510;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Involved in transcriptional regulation. Component of the NC2
CC complex which represses RNA polymerase II transcription through binding
CC to tbp and thereby inhibiting the assembly of the preinitiation complex
CC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the NC2 alpha/DRAP1 family. {ECO:0000305}.
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DR EMBL; AAFI02000190; EAL61234.1; -; Genomic_DNA.
DR RefSeq; XP_629595.1; XM_629593.1.
DR AlphaFoldDB; Q54DA1; -.
DR SMR; Q54DA1; -.
DR STRING; 44689.DDB0220098; -.
DR PaxDb; Q54DA1; -.
DR EnsemblProtists; EAL61234; EAL61234; DDB_G0292510.
DR GeneID; 8628655; -.
DR KEGG; ddi:DDB_G0292510; -.
DR dictyBase; DDB_G0292510; drap1.
DR eggNOG; KOG1659; Eukaryota.
DR HOGENOM; CLU_495616_0_0_1; -.
DR InParanoid; Q54DA1; -.
DR OMA; HLKECIK; -.
DR PRO; PR:Q54DA1; -.
DR Proteomes; UP000002195; Chromosome 6.
DR GO; GO:0017054; C:negative cofactor 2 complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0001046; F:core promoter sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; IBA:GO_Central.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0003714; F:transcription corepressor activity; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR027116; Bur6/Dpb3.
DR InterPro; IPR003958; CBFA_NFYB_domain.
DR InterPro; IPR009072; Histone-fold.
DR PANTHER; PTHR10252:SF5; PTHR10252:SF5; 1.
DR Pfam; PF00808; CBFD_NFYB_HMF; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
PE 3: Inferred from homology;
KW DNA-binding; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..550
FT /note="Dr1-associated corepressor homolog"
FT /id="PRO_0000327677"
FT DOMAIN 7..71
FT /note="Histone-fold"
FT REGION 89..355
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 378..444
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 461..512
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 89..104
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 105..139
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 169..355
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 378..425
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 550 AA; 60893 MW; 087CC74AED0ECF4C CRC64;
MKKKYKTKFP MARIKKIMQK DEEVGKIASA TPILISQCLE LFMADLVMKT CKITQAKKGK
VISVNHLKEC IKQESTFDFL TEIVDRIPDD KNEKRGRPKK TEGEDGGEEE EEEEEEMDMG
EEEEEEEDED DDDSDEEEEE APKKGGKGSR GGKGSRGGRG GARGGASTRK VKSETSPVLK
NTTITTTTAT TTPTPTPTPN FANSPKDIQS TSLKKPSARK SNTTSPKSSP FLSSSAGSIQ
SPPLNNNNNN NNNNNNNNNN NGNFNNNNNF NNNNNNFNNN NNFNNNNFNN NNNNNNFNNN
SNNNNNNFNN NNFNNNNYNS NEDENNNNNN NNNNKNNNNN NNSNNSNNNN RGQQFLSSST
ASTITLPSNQ YQPLAPISLP VLNNSNNNNS SNNNNNNNNN NNNNNNNNNE HILGNLNNNN
SNRKINTNFD EEDSNHNINK SNINSIMNSS NNSIDNKDNG FNSFTFSNNN SNFNDNNNYN
PNNPNNNNDN NGNGIVLPSL SSTNSSSTLP SFSSVPPILN TNNSNSNNSI SHILNNLNSK
KPNVEDEDFD
