NCA2_YEAST
ID NCA2_YEAST Reviewed; 616 AA.
AC Q12374; D6W4F1;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Nuclear control of ATPase protein 2;
GN Name=NCA2; OrderedLocusNames=YPR155C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=7723016; DOI=10.1016/s0022-2836(05)80140-8;
RA Camougrand N., Pelissier P., Velours G., Guerin M.;
RT "NCA2, a second nuclear gene required for the control of mitochondrial
RT synthesis of subunits 6 and 8 of ATP synthase in Saccharomyces
RT cerevisiae.";
RL J. Mol. Biol. 247:588-596(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 76625 / YPH499;
RX PubMed=14576278; DOI=10.1073/pnas.2135385100;
RA Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E.,
RA Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P.,
RA Pfanner N., Meisinger C.;
RT "The proteome of Saccharomyces cerevisiae mitochondria.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-29, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- FUNCTION: Involved in the mitochondrial expression of subunits 6 and 8
CC of the F0-F1 ATP synthase. {ECO:0000269|PubMed:7723016}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane
CC {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:14576278}; Multi-pass
CC membrane protein {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:14576278}.
CC -!- MISCELLANEOUS: Present with 2940 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the NCA2 family. {ECO:0000305}.
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DR EMBL; L20785; AAA74401.1; -; Genomic_DNA.
DR EMBL; U28371; AAB68052.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11567.1; -; Genomic_DNA.
DR PIR; S54389; S54389.
DR RefSeq; NP_015481.1; NM_001184252.1.
DR AlphaFoldDB; Q12374; -.
DR BioGRID; 36322; 78.
DR DIP; DIP-3825N; -.
DR IntAct; Q12374; 1.
DR MINT; Q12374; -.
DR STRING; 4932.YPR155C; -.
DR iPTMnet; Q12374; -.
DR MaxQB; Q12374; -.
DR PaxDb; Q12374; -.
DR PRIDE; Q12374; -.
DR EnsemblFungi; YPR155C_mRNA; YPR155C; YPR155C.
DR GeneID; 856278; -.
DR KEGG; sce:YPR155C; -.
DR SGD; S000006359; NCA2.
DR VEuPathDB; FungiDB:YPR155C; -.
DR eggNOG; ENOG502QTT6; Eukaryota.
DR HOGENOM; CLU_008227_2_0_1; -.
DR InParanoid; Q12374; -.
DR OMA; NRIYHVY; -.
DR BioCyc; YEAST:G3O-34286-MON; -.
DR PRO; PR:Q12374; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q12374; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005741; C:mitochondrial outer membrane; HDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0009060; P:aerobic respiration; IMP:SGD.
DR GO; GO:0016071; P:mRNA metabolic process; IMP:SGD.
DR InterPro; IPR013946; NCA2.
DR PANTHER; PTHR28234; PTHR28234; 1.
DR Pfam; PF08637; NCA2; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..616
FT /note="Nuclear control of ATPase protein 2"
FT /id="PRO_0000096754"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 488..508
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 29
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
SQ SEQUENCE 616 AA; 70862 MW; D56A38AF14ACF494 CRC64;
MIINRRILKS FEEISHSLEE SLREVAFDSQ QQLIQDVREE NEELSRLQDQ LQLIRSIVEK
ICISIKTDNI DSYCSVPFDL LYNICKDIAD PSSFEDGDLQ YLVSQAIFEY IILLCYYSVT
NECVQGLPAV YEAEQYYKTV SDSILKSFLY CLQNSVSTIR LLSQTVLKDV NKKKLSHQKW
SLKALSVDLL EKIRPRINKF MVIRNFRFVG LPKKPIEIAS LVSDIPRGIV HERLDMVTQS
SKYYTIKLGQ LITEFDQQPE ENGMFTEVHL PNYERRLKSL QDFFGLAMSD SNLLDVIRCS
AKFHKDHPLR RFTKPSILTR YWPSILLCLL YGPSSVMSLW NSRYFIQDFI KTNVVDFAKG
LILNWLWAPL KQVWSTVKHD EGSAISVTSQ ETLNSDMDSL TRMIVSFVVD NSDSTSNSPI
DPILLSTKVE HGDLTEFMEI YETQLHHPIK NIATGGLVRS LLIQLQKTKV DGSMALNGID
KMLKSQQLVF GVVALSPALV ILYSSIVALK RFVKLGNVWS NEKRYREQIS ISLNNVERVL
NYSKQGADAD EEHLNQGLLV IEVSNLYKLG SFLIPRSRKK EWFRDVEELV DTNLDSGAHI
NVVNRIYHVY GRFLIH
