NCALD_HUMAN
ID NCALD_HUMAN Reviewed; 193 AA.
AC P61601; P29554; Q8IYC3; Q9H0W2;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Neurocalcin-delta;
GN Name=NCALD;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Fetal brain;
RX PubMed=11267673; DOI=10.1016/s0167-4781(00)00290-6;
RA Wang W., Zhou Z., Zhao W., Huang Y., Tang R., Ying K., Xie Y., Mao Y.;
RT "Molecular cloning, mapping and characterization of the human neurocalcin
RT delta gene (NCALD).";
RL Biochim. Biophys. Acta 1518:162-167(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May be involved in the calcium-dependent regulation of
CC rhodopsin phosphorylation. Binds three calcium ions.
CC -!- SUBUNIT: Interacts with GUCY2D. {ECO:0000250|UniProtKB:Q5PQN0}.
CC -!- INTERACTION:
CC P61601; P23352: ANOS1; NbExp=3; IntAct=EBI-749635, EBI-5272188;
CC P61601; O95236-2: APOL3; NbExp=3; IntAct=EBI-749635, EBI-11976321;
CC P61601; Q9H6X5-2: C19orf44; NbExp=3; IntAct=EBI-749635, EBI-12061599;
CC P61601; Q9BXJ5: C1QTNF2; NbExp=12; IntAct=EBI-749635, EBI-2817707;
CC P61601; Q03060-25: CREM; NbExp=5; IntAct=EBI-749635, EBI-12884642;
CC P61601; Q86UW9: DTX2; NbExp=12; IntAct=EBI-749635, EBI-740376;
CC P61601; P10767: FGF6; NbExp=3; IntAct=EBI-749635, EBI-11479013;
CC P61601; O75084: FZD7; NbExp=3; IntAct=EBI-749635, EBI-746917;
CC P61601; Q7Z5G4: GOLGA7; NbExp=3; IntAct=EBI-749635, EBI-4403685;
CC P61601; Q96SL4: GPX7; NbExp=3; IntAct=EBI-749635, EBI-749411;
CC P61601; P24592: IGFBP6; NbExp=3; IntAct=EBI-749635, EBI-947015;
CC P61601; P02538: KRT6A; NbExp=3; IntAct=EBI-749635, EBI-702198;
CC P61601; Q5TA82: LCE2D; NbExp=3; IntAct=EBI-749635, EBI-10246750;
CC P61601; P15018: LIF; NbExp=3; IntAct=EBI-749635, EBI-1037189;
CC P61601; Q99732: LITAF; NbExp=5; IntAct=EBI-749635, EBI-725647;
CC P61601; O95868: LY6G6D; NbExp=3; IntAct=EBI-749635, EBI-12382527;
CC P61601; P50222: MEOX2; NbExp=3; IntAct=EBI-749635, EBI-748397;
CC P61601; Q96C03-3: MIEF2; NbExp=3; IntAct=EBI-749635, EBI-11988931;
CC P61601; P55197-2: MLLT10; NbExp=3; IntAct=EBI-749635, EBI-12853322;
CC P61601; Q969H8: MYDGF; NbExp=5; IntAct=EBI-749635, EBI-718622;
CC P61601; P41271-2: NBL1; NbExp=3; IntAct=EBI-749635, EBI-12135485;
CC P61601; Q9NWW9: PLAAT2; NbExp=3; IntAct=EBI-749635, EBI-12253270;
CC P61601; Q6UWP8-2: SBSN; NbExp=3; IntAct=EBI-749635, EBI-18282351;
CC P61601; Q7Z699: SPRED1; NbExp=9; IntAct=EBI-749635, EBI-5235340;
CC P61601; P10124: SRGN; NbExp=3; IntAct=EBI-749635, EBI-744915;
CC P61601; Q9NRR2: TPSG1; NbExp=3; IntAct=EBI-749635, EBI-17210651;
CC P61601; Q7DB77: tir; Xeno; NbExp=3; IntAct=EBI-749635, EBI-6480811;
CC -!- TISSUE SPECIFICITY: Retina, cerebrum, cerebellum, brain stem, spinal
CC cord, testis, ovary and small intestine.
CC -!- SIMILARITY: Belongs to the recoverin family. {ECO:0000305}.
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DR EMBL; AF251061; AAK34951.1; -; mRNA.
DR EMBL; AL136612; CAB66547.1; -; mRNA.
DR EMBL; BC036098; AAH36098.1; -; mRNA.
DR EMBL; BC063428; AAH63428.1; -; mRNA.
DR CCDS; CCDS6292.1; -.
DR RefSeq; NP_001035714.1; NM_001040624.1.
DR RefSeq; NP_001035715.1; NM_001040625.1.
DR RefSeq; NP_001035716.1; NM_001040626.1.
DR RefSeq; NP_001035717.1; NM_001040627.1.
DR RefSeq; NP_001035718.1; NM_001040628.1.
DR RefSeq; NP_001035719.1; NM_001040629.1.
DR RefSeq; NP_001035720.1; NM_001040630.1.
DR RefSeq; NP_114430.2; NM_032041.2.
DR RefSeq; XP_011515634.1; XM_011517332.2.
DR RefSeq; XP_011515635.1; XM_011517333.2.
DR RefSeq; XP_011515636.1; XM_011517334.2.
DR RefSeq; XP_011515637.1; XM_011517335.2.
DR RefSeq; XP_016869390.1; XM_017013901.1.
DR RefSeq; XP_016869391.1; XM_017013902.1.
DR AlphaFoldDB; P61601; -.
DR SMR; P61601; -.
DR BioGRID; 123839; 49.
DR IntAct; P61601; 37.
DR MINT; P61601; -.
DR STRING; 9606.ENSP00000379256; -.
DR iPTMnet; P61601; -.
DR PhosphoSitePlus; P61601; -.
DR BioMuta; NCALD; -.
DR DMDM; 47606440; -.
DR EPD; P61601; -.
DR jPOST; P61601; -.
DR MassIVE; P61601; -.
DR MaxQB; P61601; -.
DR PaxDb; P61601; -.
DR PeptideAtlas; P61601; -.
DR PRIDE; P61601; -.
DR ProteomicsDB; 57324; -.
DR Antibodypedia; 42836; 178 antibodies from 27 providers.
DR DNASU; 83988; -.
DR Ensembl; ENST00000220931.11; ENSP00000220931.6; ENSG00000104490.18.
DR Ensembl; ENST00000311028.4; ENSP00000310587.3; ENSG00000104490.18.
DR Ensembl; ENST00000395923.5; ENSP00000379256.1; ENSG00000104490.18.
DR Ensembl; ENST00000519508.6; ENSP00000430476.1; ENSG00000104490.18.
DR Ensembl; ENST00000521599.5; ENSP00000428105.1; ENSG00000104490.18.
DR GeneID; 83988; -.
DR KEGG; hsa:83988; -.
DR MANE-Select; ENST00000220931.11; ENSP00000220931.6; NM_032041.3; NP_114430.2.
DR UCSC; uc003yke.4; human.
DR CTD; 83988; -.
DR DisGeNET; 83988; -.
DR GeneCards; NCALD; -.
DR HGNC; HGNC:7655; NCALD.
DR HPA; ENSG00000104490; Tissue enhanced (salivary).
DR MIM; 606722; gene.
DR neXtProt; NX_P61601; -.
DR OpenTargets; ENSG00000104490; -.
DR PharmGKB; PA31458; -.
DR VEuPathDB; HostDB:ENSG00000104490; -.
DR eggNOG; KOG0044; Eukaryota.
DR GeneTree; ENSGT00940000158862; -.
DR HOGENOM; CLU_072366_1_0_1; -.
DR InParanoid; P61601; -.
DR OMA; MGCVCMK; -.
DR PhylomeDB; P61601; -.
DR TreeFam; TF300009; -.
DR PathwayCommons; P61601; -.
DR Reactome; R-HSA-451308; Activation of Ca-permeable Kainate Receptor.
DR SignaLink; P61601; -.
DR BioGRID-ORCS; 83988; 8 hits in 1075 CRISPR screens.
DR ChiTaRS; NCALD; human.
DR GenomeRNAi; 83988; -.
DR Pharos; P61601; Tbio.
DR PRO; PR:P61601; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; P61601; protein.
DR Bgee; ENSG00000104490; Expressed in Brodmann (1909) area 10 and 150 other tissues.
DR ExpressionAtlas; P61601; baseline and differential.
DR Genevisible; P61601; HS.
DR GO; GO:0030130; C:clathrin coat of trans-Golgi network vesicle; NAS:UniProtKB.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0003779; F:actin binding; IDA:UniProtKB.
DR GO; GO:0043014; F:alpha-tubulin binding; IEA:Ensembl.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0030276; F:clathrin binding; IDA:UniProtKB.
DR GO; GO:0015631; F:tubulin binding; IDA:UniProtKB.
DR GO; GO:0019722; P:calcium-mediated signaling; IBA:GO_Central.
DR GO; GO:0003073; P:regulation of systemic arterial blood pressure; IEA:Ensembl.
DR GO; GO:0016192; P:vesicle-mediated transport; NAS:UniProtKB.
DR CDD; cd00051; EFh; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR028846; Recoverin.
DR PANTHER; PTHR23055; PTHR23055; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR Pfam; PF13833; EF-hand_8; 1.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 3.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 1: Evidence at protein level;
KW Calcium; Lipoprotein; Metal-binding; Myristate; Reference proteome; Repeat.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..193
FT /note="Neurocalcin-delta"
FT /id="PRO_0000073782"
FT DOMAIN 40..58
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 60..95
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 96..131
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 144..179
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 73
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 75
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 77
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 79
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 84
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 109
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 111
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 113
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 115
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 120
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 157
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 159
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 161
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 163
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 168
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000250"
FT CONFLICT 123
FT /note="E -> V (in Ref. 2; CAB66547)"
FT /evidence="ECO:0000305"
FT CONFLICT 166
FT /note="L -> M (in Ref. 3; AAH36098)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 193 AA; 22245 MW; 20DD8B6FF47C4CBF CRC64;
MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK IYGNFFPYGD
ASKFAEHVFR TFDANGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISKAE
MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNR DGKLSLEEFI RGAKSDPSIV
RLLQCDPSSA GQF
