NCAP_BDVV
ID NCAP_BDVV Reviewed; 370 AA.
AC P0C797; Q01552;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 08-MAR-2011, sequence version 1.
DT 23-FEB-2022, entry version 51.
DE RecName: Full=Nucleoprotein;
DE Short=N protein;
DE AltName: Full=Nucleocapsid protein;
DE AltName: Full=p38;
DE AltName: Full=p40;
GN Name=N;
OS Borna disease virus (strain V) (BDV).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Bornaviridae; unclassified Bornaviridae.
OX NCBI_TaxID=928296;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
OH NCBI_TaxID=9352; Bradypodidae (three-fingered sloths).
OH NCBI_TaxID=9925; Capra hircus (Goat).
OH NCBI_TaxID=9850; Cervidae (deer).
OH NCBI_TaxID=109474; Crocidura leucodon (Bicoloured white-toothed shrew) (Celebes shrew).
OH NCBI_TaxID=9788; Equidae (horses).
OH NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH NCBI_TaxID=56798; Hexaprotodon liberiensis (Pygmy hippopotamus) (Choeropsis liberiensis).
OH NCBI_TaxID=9844; Lama glama (Llama).
OH NCBI_TaxID=9986; Oryctolagus cuniculus (Rabbit).
OH NCBI_TaxID=9940; Ovis aries (Sheep).
OH NCBI_TaxID=8801; Struthio camelus (Common ostrich).
OH NCBI_TaxID=9455; Varecia variegata (Black-and-white ruffed lemur) (Lemur variegatus).
OH NCBI_TaxID=30538; Vicugna pacos (Alpaca) (Lama pacos).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=8183914; DOI=10.1073/pnas.91.10.4362;
RA Briese T., Schneemann A., Lewis A.J., Park Y.-S., Kim S., Ludwig H.,
RA Lipkin W.I.;
RT "Genomic organization of Borna disease virus.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:4362-4366(1994).
RN [2]
RP INTERACTION WITH PHOSPHOPROTEIN P.
RX PubMed=9880009; DOI=10.1099/0022-1317-79-12-2957;
RA Berg M., Ehrenborg C., Blomberg J., Pipkorn R., Berg A.L.;
RT "Two domains of the Borna disease virus p40 protein are required for
RT interaction with the p23 protein.";
RL J. Gen. Virol. 79:2957-2963(1998).
RN [3]
RP SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=11238866; DOI=10.1128/jvi.75.7.3404-3412.2001;
RA Kobayashi T., Kamitani W., Zhang G., Watanabe M., Tomonaga K., Ikuta K.;
RT "Borna disease virus nucleoprotein requires both nuclear localization and
RT export activities for viral nucleocytoplasmic shuttling.";
RL J. Virol. 75:3404-3412(2001).
RN [4]
RP FUNCTION, AND INTERACTION WITH HOST CDK1.
RX PubMed=14512566; DOI=10.1128/jvi.77.20.11186-11192.2003;
RA Planz O., Pleschka S., Oesterle K., Berberich-Siebelt F., Ehrhardt C.,
RA Stitz L., Ludwig S.;
RT "Borna disease virus nucleoprotein interacts with the CDC2-cyclin B1
RT complex.";
RL J. Virol. 77:11186-11192(2003).
RN [5]
RP SUBUNIT.
RX PubMed=19945724; DOI=10.1016/j.virol.2009.11.016;
RA Hock M., Kraus I., Schoehn G., Jamin M., Andrei-Selmer C., Garten W.,
RA Weissenhorn W.;
RT "RNA induced polymerization of the Borna disease virus nucleoprotein.";
RL Virology 397:64-72(2010).
RN [6]
RP FUNCTION, AND INTERACTION WITH HOST NFKB1.
RX PubMed=25733193; DOI=10.1038/srep08696;
RA Makino A., Fujino K., Parrish N.F., Honda T., Tomonaga K.;
RT "Borna disease virus possesses an NF-kB inhibitory sequence in the
RT nucleoprotein gene.";
RL Sci. Rep. 5:8696-8696(2015).
CC -!- FUNCTION: Encapsidates the genome, protecting it from nucleases. The
CC encapsidated genomic RNA is termed the NC and serves as template for
CC transcription and replication. Plays a critical role in the
CC nucleocytoplasmic transport of viral RNP by interaction with other
CC viral proteins. The nuclear export signal is masked by the interaction
CC with the phosphoprotein P. Delays the host cell cycle progression by
CC interacting with the host CDK1-cyclin B1 phase in late G2. Inhibits
CC host NF-kappaB activation. {ECO:0000269|PubMed:11238866,
CC ECO:0000269|PubMed:14512566, ECO:0000269|PubMed:25733193}.
CC -!- SUBUNIT: Homomultimerizes to form the nucleocapsid (PubMed:19945724).
CC Binds to viral genomic RNA (PubMed:19945724). Interacts with
CC phosphoprotein P (PubMed:9880009, PubMed:19945724). Interacts with host
CC CDK1 (PubMed:14512566); this interaction delays host cell cycle in late
CC G2. Interacts with host NFKB1 (PubMed:25733193).
CC {ECO:0000269|PubMed:14512566, ECO:0000269|PubMed:25733193,
CC ECO:0000269|PubMed:9880009}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host nucleus
CC {ECO:0000269|PubMed:11238866}. Host cytoplasm
CC {ECO:0000269|PubMed:11238866}. Note=Shuttles between the host nucleus
CC and cytoplasm. {ECO:0000269|PubMed:11238866}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=p40;
CC IsoId=P0C797-1; Sequence=Displayed;
CC Name=p38;
CC IsoId=P0C797-2; Sequence=VSP_040675;
CC -!- MISCELLANEOUS: Isoform P38 does not have the nuclear localization
CC signal, nonetheless it is localized in the nucleus. {ECO:0000250}.
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DR EMBL; U04608; AAA20224.1; -; Genomic_RNA.
DR RefSeq; NP_042020.1; NC_001607.1.
DR SMR; P0C797; -.
DR IntAct; P0C797; 2.
DR GeneID; 26799168; -.
DR KEGG; vg:26799168; -.
DR Proteomes; UP000007804; Genome.
DR GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0030430; C:host cell cytoplasm; IDA:UniProtKB.
DR GO; GO:0042025; C:host cell nucleus; IDA:UniProtKB.
DR GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR GO; GO:0019055; P:modification by virus of host cell cycle regulation; IDA:UniProtKB.
DR GO; GO:0039592; P:suppression by virus of G2/M transition of host mitotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0039644; P:suppression by virus of host NF-kappaB cascade; IDA:UniProtKB.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0075732; P:viral penetration into host nucleus; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3040.10; -; 1.
DR Gene3D; 1.10.3050.10; -; 1.
DR InterPro; IPR009441; P40_nucleoprot_BD-vir.
DR InterPro; IPR036260; P40_nucleoprot_sf_BD-vir.
DR InterPro; IPR015969; P40_nucleoprot_sub1_BD-vir.
DR InterPro; IPR015970; P40_nucleoprot_sub2_BD-vir.
DR Pfam; PF06407; BDV_P40; 1.
DR SUPFAM; SSF101399; SSF101399; 1.
PE 1: Evidence at protein level;
KW Alternative initiation; Capsid protein; Helical capsid protein;
KW Host cytoplasm; Host G2/M cell cycle arrest by virus; Host nucleus;
KW Host-virus interaction; Inhibition of host NF-kappa-B by virus;
KW Modulation of host cell cycle by virus; Reference proteome;
KW Viral nucleoprotein; Viral penetration into host nucleus; Virion;
KW Virus entry into host cell.
FT CHAIN 1..370
FT /note="Nucleoprotein"
FT /id="PRO_0000405344"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 3..11
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:11238866"
FT MOTIF 128..141
FT /note="Nuclear export signal"
FT /evidence="ECO:0000269|PubMed:11238866"
FT VAR_SEQ 1..13
FT /note="Missing (in isoform p38)"
FT /evidence="ECO:0000305"
FT /id="VSP_040675"
SQ SEQUENCE 370 AA; 40939 MW; C5A8FFA71AF4FE52 CRC64;
MPPKRRLVDD ADAMEDQDLY EPPASLPKLP GKFLQYTVGG SDPHPGIGHE KDIRQNAVAL
LDQSRRDMFH TVTPSLVFLC LLIPGLHAAF VHGGVPRESY LSTPVTRGEQ TVVKTAKFYG
EKTTQRDLTE LEISSIFSHC CSLLIGVVIG SSSKIKAGAE QIKKRFKTMM AALNRPSHGE
TATLLQMFNP HEAIDWINGQ PWVGSFVLSL LTTDFESPGK EFMDQIKLVA SYAQMTTYTT
IKEYLAECMD ATLTIPVVAY EIRDFLEVSA KLKEDHADLF PFLGAIRHPD AIKLAPRSFP
NLASAAFYWS KKENPTMAGY RASTIQPGAS VKETQLARYR RREISRGEDG AELSGEISAI
MKMIGVTGLN
