ID   NCAP_BEFVB              Reviewed;         431 AA.
AC   Q65474;
DT   21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   23-FEB-2022, entry version 83.
DE   RecName: Full=Nucleoprotein;
DE            Short=NP;
DE   AltName: Full=Nucleocapsid protein;
DE            Short=Protein N;
GN   Name=N;
OS   Bovine ephemeral fever virus (strain BB7721) (BEFV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae;
OC   Ephemerovirus.
OX   NCBI_TaxID=928297;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
OH   NCBI_TaxID=89462; Bubalus bubalis (Domestic water buffalo).
OH   NCBI_TaxID=58271; Culicoides.
OH   NCBI_TaxID=9970; Syncerus caffer (African buffalo).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8046391; DOI=10.1099/0022-1317-75-8-1889;
RA   Walker P.J., Wang Y., Cowley J.A., McWilliam S.M., Prehaud C.J.;
RT   "Structural and antigenic analysis of the nucleoprotein of bovine ephemeral
RT   fever rhabdovirus.";
RL   J. Gen. Virol. 75:1889-1899(1994).
CC   -!- FUNCTION: Encapsidates the genome, protecting it from nucleases. If
CC       expressed without protein P it binds non-specifically RNA and therefore
CC       can bind it's own mRNA. Interaction with protein P abolishes any non-
CC       specific RNA binding, and prevents phosphorylation. The soluble N-P
CC       complex encapsidates specifically the genomic RNA, with protein N
CC       protecting the genome like a pearl necklace. The encapsidated genomic
CC       RNA is termed the nucleocapsid (NC) and serves as template for viral
CC       transcription and replication. Protein N binds protein P in the NC
CC       through a different interaction, and can be phosphorylated. Subsequent
CC       viral replication is dependent on intracellular concentration of newly
CC       synthesized protein N. During replication, encapsidation by protein N
CC       is coupled to RNA synthesis and all replicative products are resistant
CC       to nucleases (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Binds to viral
CC       genomic RNA (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ephemerovirus nucleocapsid protein family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U04166; AAA19450.1; -; Genomic_RNA.
DR   EMBL; AF234533; AAG10409.1; -; Genomic_RNA.
DR   RefSeq; NP_065398.1; NC_002526.1.
DR   SMR; Q65474; -.
DR   GeneID; 911726; -.
DR   KEGG; vg:911726; -.
DR   Proteomes; UP000008588; Genome.
DR   GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.3570.10; -; 1.
DR   Gene3D; 1.10.3610.10; -; 1.
DR   InterPro; IPR000448; Rhabdo_ncapsid.
DR   InterPro; IPR023331; Rhabdovirus_ncapsid_C.
DR   InterPro; IPR023330; Rhabdovirus_ncapsid_N.
DR   InterPro; IPR035961; Rhabdovirus_nucleoprotein-like.
DR   Pfam; PF00945; Rhabdo_ncap; 1.
DR   SUPFAM; SSF140809; SSF140809; 1.
PE   3: Inferred from homology;
KW   Capsid protein; Helical capsid protein; Host cytoplasm; Phosphoprotein;
KW   Reference proteome; Ribonucleoprotein; RNA-binding; Viral nucleoprotein;
KW   Virion.
FT   CHAIN           1..431
FT                   /note="Nucleoprotein"
FT                   /id="PRO_0000297608"
FT   REGION          352..379
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        360..375
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   431 AA;  49163 MW;  906A6D17C11BA2ED CRC64;
     MYCTLNKKEI KAVKPTDAIP PQYPKEFFIN GNGKKPTLRV PQGKLDLPTV RELVFGGLER
     GELVLSHVIR YLYLVGERIT EKLEGDWISF GVNIGRRNQE INVWNFYEVI IEDDQTIDGR
     RANNVDENDD VWLTLALLAY YRLGRSANQN HRNNLLIKLN AQIKGYRKDA PNIIDDVAVH
     GSWVTNSEFC KIAAGFDMFM NRFKNNKYAH VRFGTVASRY KDAAGLMALG HACDVTGLTI
     GEILDWIFVS NVGEDVVKIM EEGNEIDDPY SYMPYMMDMG ISNKSPYSSI SCPHIYTFLH
     LVGTLLTSER SKHARMVSEH NLQNIKMNAF VVSYVKSNKA ALTKAFLKSE DRDYEKRQED
     GSNDDEDEDE SGDDDDFGAM PKSSDPMEWF IFLESNHFIL PEKVTEFCIR ECKKIQNARP
     NTIGKYLASI V