NCAP_BUNYW
ID NCAP_BUNYW Reviewed; 233 AA.
AC P16495;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 29-SEP-2021, entry version 81.
DE RecName: Full=Nucleoprotein;
DE AltName: Full=Nucleocapsid protein;
DE Short=Protein N;
GN Name=N;
OS Bunyamwera virus (BUNV).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Ellioviricetes; Bunyavirales; Peribunyaviridae; Orthobunyavirus.
OX NCBI_TaxID=35304;
OH NCBI_TaxID=7159; Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2732714; DOI=10.1099/0022-1317-70-5-1281;
RA Elliott R.M.;
RT "Nucleotide sequence analysis of the small (S) RNA segment of Bunyamwera
RT virus, the prototype of the family Bunyaviridae.";
RL J. Gen. Virol. 70:1281-1285(1989).
RN [2]
RP FUNCTION, AND INTERACTION WITH HOST PABP1.
RX PubMed=19193790; DOI=10.1128/jvi.02201-08;
RA Blakqori G., van Knippenberg I., Elliott R.M.;
RT "Bunyamwera orthobunyavirus S-segment untranslated regions mediate poly(A)
RT tail-independent translation.";
RL J. Virol. 83:3637-3646(2009).
CC -!- FUNCTION: Encapsidates the genome protecting it from nucleases. The
CC encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as
CC template for transcription and replication. Seems to participate in the
CC nuclear relocalization of host PABP1, thereby inhibiting host cellular
CC translation. {ECO:0000269|PubMed:19193790}.
CC -!- SUBUNIT: Homomultimerizes to form the nucleocapsid (By similarity).
CC Binds to viral genomic RNA (By similarity). Interacts with host PABP1.
CC {ECO:0000250, ECO:0000269|PubMed:19193790}.
CC -!- SUBCELLULAR LOCATION: Virion. Note=Internal protein of virus particle.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=N;
CC IsoId=P16495-1; Sequence=Displayed;
CC Name=NSS;
CC IsoId=P16494-1; Sequence=External;
CC -!- SIMILARITY: Belongs to the orthobunyavirus nucleocapsid protein family.
CC {ECO:0000305}.
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DR EMBL; D00353; BAA00261.1; -; Genomic_RNA.
DR PIR; A31365; VHVUBV.
DR RefSeq; NP_047213.1; NC_001927.1. [P16495-1]
DR PDB; 3ZLA; X-ray; 3.20 A; A/B/C/D/E/F/G/H=1-233.
DR PDB; 4IJS; X-ray; 3.20 A; A/B/C/D=2-233.
DR PDBsum; 3ZLA; -.
DR PDBsum; 4IJS; -.
DR SMR; P16495; -.
DR GeneID; 2648217; -.
DR KEGG; vg:2648217; -.
DR Proteomes; UP000002476; Genome.
DR GO; GO:0019029; C:helical viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0039657; P:suppression by virus of host gene expression; IEA:UniProtKB-KW.
DR Gene3D; 1.10.472.180; -; 1.
DR Gene3D; 1.20.142.20; -; 1.
DR InterPro; IPR001784; Bunya_nucleocap.
DR InterPro; IPR043011; Bunya_nucleocap_C.
DR InterPro; IPR043012; Bunya_nucleocap_N.
DR Pfam; PF00952; Bunya_nucleocap; 1.
DR PIRSF; PIRSF003947; N_OrthobunV; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative initiation; Capsid protein;
KW Eukaryotic host gene expression shutoff by virus;
KW Eukaryotic host translation shutoff by virus; Helical capsid protein;
KW Host gene expression shutoff by virus; Host-virus interaction;
KW Reference proteome; Ribonucleoprotein; RNA-binding; Viral nucleoprotein;
KW Virion.
FT CHAIN 1..233
FT /note="Nucleoprotein"
FT /id="PRO_0000221992"
FT STRAND 1..6
FT /evidence="ECO:0007829|PDB:3ZLA"
FT HELIX 19..29
FT /evidence="ECO:0007829|PDB:3ZLA"
FT STRAND 31..33
FT /evidence="ECO:0007829|PDB:3ZLA"
FT HELIX 36..44
FT /evidence="ECO:0007829|PDB:3ZLA"
FT HELIX 46..55
FT /evidence="ECO:0007829|PDB:3ZLA"
FT STRAND 59..65
FT /evidence="ECO:0007829|PDB:3ZLA"
FT STRAND 68..73
FT /evidence="ECO:0007829|PDB:3ZLA"
FT HELIX 78..80
FT /evidence="ECO:0007829|PDB:3ZLA"
FT HELIX 92..109
FT /evidence="ECO:0007829|PDB:3ZLA"
FT HELIX 112..121
FT /evidence="ECO:0007829|PDB:3ZLA"
FT HELIX 125..129
FT /evidence="ECO:0007829|PDB:3ZLA"
FT HELIX 134..136
FT /evidence="ECO:0007829|PDB:3ZLA"
FT HELIX 138..143
FT /evidence="ECO:0007829|PDB:3ZLA"
FT HELIX 148..151
FT /evidence="ECO:0007829|PDB:3ZLA"
FT TURN 152..157
FT /evidence="ECO:0007829|PDB:3ZLA"
FT HELIX 158..167
FT /evidence="ECO:0007829|PDB:3ZLA"
FT TURN 168..170
FT /evidence="ECO:0007829|PDB:3ZLA"
FT HELIX 174..177
FT /evidence="ECO:0007829|PDB:3ZLA"
FT HELIX 180..182
FT /evidence="ECO:0007829|PDB:3ZLA"
FT HELIX 190..196
FT /evidence="ECO:0007829|PDB:3ZLA"
FT HELIX 198..210
FT /evidence="ECO:0007829|PDB:3ZLA"
FT STRAND 215..217
FT /evidence="ECO:0007829|PDB:3ZLA"
FT HELIX 220..227
FT /evidence="ECO:0007829|PDB:3ZLA"
FT TURN 228..230
FT /evidence="ECO:0007829|PDB:4IJS"
SQ SEQUENCE 233 AA; 26664 MW; D44C80C93FA8A066 CRC64;
MIELEFHDVA ANTSSTFDPE VAYANFKRVH TTGLSYDHIR IFYIKGREIK TSLAKRSEWE
VTLNLGGWKI TVYNTNFPGN RNNPVPDDGL TLHRLSGFLA RYLLEKMLKV SEPEKLIIKS
KIINPLAEKN GITWNDGEEV YLSFFPGSEM FLGTFRFYPL AIGIYKVQRK EMEPKYLEKT
MRQRYMGLEA ATWTVSKLTE VQSALTVVSS LGWKKTNVSA AARDFLAKFG INM
