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NCAP_CVPFS
ID   NCAP_CVPFS              Reviewed;         382 AA.
AC   P05991;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   02-JUN-2021, entry version 84.
DE   RecName: Full=Nucleoprotein {ECO:0000255|HAMAP-Rule:MF_04095};
DE   AltName: Full=Nucleocapsid protein {ECO:0000255|HAMAP-Rule:MF_04095};
DE            Short=NC {ECO:0000255|HAMAP-Rule:MF_04095};
DE            Short=Protein N {ECO:0000255|HAMAP-Rule:MF_04095};
GN   Name=N {ECO:0000255|HAMAP-Rule:MF_04095}; ORFNames=6;
OS   Porcine transmissible gastroenteritis coronavirus (strain FS772/70) (TGEV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Alphacoronavirus; Tegacovirus.
OX   NCBI_TaxID=11150;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2835592; DOI=10.1111/j.1365-2958.1988.tb00010.x;
RA   Britton P., Carmenes R.S., Page K.W., Garwes D.J., Parra F.;
RT   "Sequence of the nucleoprotein gene from a virulent British field isolate
RT   of transmissible gastroenteritis virus and its expression in Saccharomyces
RT   cerevisiae.";
RL   Mol. Microbiol. 2:89-99(1988).
RN   [2]
RP   SUBCELLULAR LOCATION.
RX   PubMed=11533198; DOI=10.1128/jvi.75.19.9345-9356.2001;
RA   Wurm T., Chen H., Hodgson T., Britton P., Brooks G., Hiscox J.A.;
RT   "Localization to the nucleolus is a common feature of coronavirus
RT   nucleoproteins, and the protein may disrupt host cell division.";
RL   J. Virol. 75:9345-9356(2001).
CC   -!- FUNCTION: Packages the positive strand viral genome RNA into a helical
CC       ribonucleocapsid (RNP) and plays a fundamental role during virion
CC       assembly through its interactions with the viral genome and membrane
CC       protein M. Plays an important role in enhancing the efficiency of
CC       subgenomic viral RNA transcription as well as viral replication.
CC       {ECO:0000255|HAMAP-Rule:MF_04095}.
CC   -!- SUBUNIT: Homooligomer. Both monomeric and oligomeric forms interact
CC       with RNA. Interacts with protein M. Interacts with NSP3; this
CC       interaction serves to tether the genome to the newly translated
CC       replicase-transcriptase complex at a very early stage of infection.
CC       {ECO:0000255|HAMAP-Rule:MF_04095}.
CC   -!- INTERACTION:
CC       P05991; P02543: VIM; Xeno; NbExp=4; IntAct=EBI-25636086, EBI-25636029;
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04095}. Host
CC       endoplasmic reticulum-Golgi intermediate compartment
CC       {ECO:0000255|HAMAP-Rule:MF_04095}. Host Golgi apparatus
CC       {ECO:0000255|HAMAP-Rule:MF_04095}. Host nucleus, host nucleolus
CC       {ECO:0000269|PubMed:11533198}. Note=Located inside the virion,
CC       complexed with the viral RNA. Probably associates with ER-derived
CC       membranes where it participates in viral RNA synthesis and virus
CC       budding. {ECO:0000255|HAMAP-Rule:MF_04095}.
CC   -!- PTM: ADP-ribosylated. The ADP-ribosylation is retained in the virion
CC       during infection. {ECO:0000255|HAMAP-Rule:MF_04095}.
CC   -!- PTM: Phosphorylated on serine and threonine residues.
CC       {ECO:0000255|HAMAP-Rule:MF_04095}.
CC   -!- SIMILARITY: Belongs to the alphacoronavirus nucleocapsid protein
CC       family. {ECO:0000255|HAMAP-Rule:MF_04095}.
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DR   EMBL; Y00542; CAA68607.1; -; Genomic_RNA.
DR   PIR; S03762; S03762.
DR   SMR; P05991; -.
DR   IntAct; P05991; 3.
DR   GO; GO:0044172; C:host cell endoplasmic reticulum-Golgi intermediate compartment; IEA:UniProtKB-SubCell.
DR   GO; GO:0044177; C:host cell Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   CDD; cd21595; CoV_N-CTD; 1.
DR   CDD; cd21554; CoV_N-NTD; 1.
DR   HAMAP; MF_04095; ALPHA_CORONA_NCAP; 1.
DR   InterPro; IPR044344; N_prot_C_CoV.
DR   InterPro; IPR044345; N_prot_N_CoV.
DR   InterPro; IPR042548; NCAP_aCoV.
DR   InterPro; IPR001218; Nucleocap_CoV.
DR   InterPro; IPR037179; Nucleocapsid_C.
DR   InterPro; IPR037195; Nucleocapsid_N.
DR   Pfam; PF00937; CoV_nucleocap; 1.
DR   PIRSF; PIRSF003888; Corona_nucleocap; 1.
DR   SUPFAM; SSF103068; SSF103068; 1.
DR   SUPFAM; SSF110304; SSF110304; 1.
DR   PROSITE; PS51929; COV_N_CTD; 1.
DR   PROSITE; PS51928; COV_N_NTD; 1.
PE   1: Evidence at protein level;
KW   ADP-ribosylation; Host Golgi apparatus; Host nucleus; Phosphoprotein;
KW   Ribonucleoprotein; RNA-binding; Transcription; Transcription regulation;
KW   Viral nucleoprotein; Virion.
FT   CHAIN           1..382
FT                   /note="Nucleoprotein"
FT                   /id="PRO_0000106012"
FT   DOMAIN          31..153
FT                   /note="CoV N NTD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01276"
FT   DOMAIN          224..337
FT                   /note="CoV N CTD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01277"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          33..159
FT                   /note="RNA-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04095"
FT   REGION          150..240
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          231..334
FT                   /note="Dimerization"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04095"
FT   REGION          329..358
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        204..240
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        334..357
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         9
FT                   /note="Phosphoserine; by host"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04095"
FT   MOD_RES         156
FT                   /note="Phosphoserine; by host"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04095"
FT   MOD_RES         254
FT                   /note="Phosphoserine; by host"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04095"
FT   MOD_RES         256
FT                   /note="Phosphoserine; by host"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04095"
SQ   SEQUENCE   382 AA;  43483 MW;  25152E1D8F327F9E CRC64;
     MANQGQRVSW GDESTKIRGR SNSRGRKSNN IPLSFFNPIT LQQGSKFWNL CPRDFVPKGI
     GNRDQQIGYW NRQTRYRMVK GQRKELPERW FFYYLGTGPH ADAKFKDKLD GVVWVAKDGA
     MNKPTTLGSR GANNESKALK FDGKVPGEFQ LEVNQSRDNS RSRSQSRSRS RNRSQSRGRQ
     QSNNKKDDSV EQAVLAALKK LGVDTEKQQQ RSCSKSKERS NSKTRDTTPK NENKHTWKRT
     AGKGDVTRFY GARSSSANFG DSDLVANGSS AKHYPQLAEC VPSVSSILFG SYWTSKEDGD
     QIEVTFTHKY HLPKDDPKTE QFLQQINAYA RPSEVAKEQR KRKSRSKSAE RSEQEVVPDA
     LIENYTDVFD DTQVEMIDEV TN
 
 
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