NCAP_CVPPU
ID NCAP_CVPPU Reviewed; 382 AA.
AC P04134; Q9IW03;
DT 01-NOV-1986, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2003, sequence version 2.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Nucleoprotein {ECO:0000255|HAMAP-Rule:MF_04095};
DE AltName: Full=Nucleocapsid protein {ECO:0000255|HAMAP-Rule:MF_04095};
DE Short=NC {ECO:0000255|HAMAP-Rule:MF_04095};
DE Short=Protein N {ECO:0000255|HAMAP-Rule:MF_04095};
GN Name=N {ECO:0000255|HAMAP-Rule:MF_04095}; ORFNames=6;
OS Porcine transmissible gastroenteritis coronavirus (strain Purdue) (TGEV).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC Alphacoronavirus; Tegacovirus.
OX NCBI_TaxID=11151;
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2825819; DOI=10.1016/0300-9084(87)90178-7;
RA Rasschaert D., Gelfi J., Laude H.;
RT "Enteric coronavirus TGEV: partial sequence of the genomic RNA, its
RT organization and expression.";
RL Biochimie 69:591-600(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=3008432; DOI=10.1016/0042-6822(86)90102-9;
RA Kapke P.A., Brian D.A.;
RT "Sequence analysis of the porcine transmissible gastroenteritis coronavirus
RT nucleocapsid protein gene.";
RL Virology 151:41-49(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate PUR46-MAD;
RX PubMed=10805807; DOI=10.1073/pnas.97.10.5516;
RA Almazan F., Gonzalez J.M., Penzes Z., Izeta A., Calvo E., Plana-Duran J.,
RA Enjuanes L.;
RT "Engineering the largest RNA virus genome as an infectious bacterial
RT artificial chromosome.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:5516-5521(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-21.
RX PubMed=2841792; DOI=10.1016/0042-6822(88)90581-8;
RA Kapke P.A., Tung F.Y.T., Hogue B.G., Brian D.A., Woods R.D., Wesley R.;
RT "The amino-terminal signal peptide on the porcine transmissible
RT gastroenteritis coronavirus matrix protein is not an absolute requirement
RT for membrane translocation and glycosylation.";
RL Virology 165:367-376(1988).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=1645905; DOI=10.1016/0168-1702(91)90019-r;
RA Pulford D.J., Britton P.;
RT "Expression and cellular localisation of porcine transmissible
RT gastroenteritis virus N and M proteins by recombinant vaccinia viruses.";
RL Virus Res. 18:203-217(1991).
RN [6]
RP FUNCTION.
RX PubMed=15507657; DOI=10.1128/jvi.78.22.12683-12688.2004;
RA Almazan F., Galan C., Enjuanes L.;
RT "The nucleoprotein is required for efficient coronavirus genome
RT replication.";
RL J. Virol. 78:12683-12688(2004).
RN [7]
RP PHOSPHORYLATION AT SER-9; SER-156; SER-254 AND SER-256, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=Isolate PUR-C11;
RX PubMed=16033973; DOI=10.1099/vir.0.80975-0;
RA Calvo E., Escors D., Lopez J.A., Gonzalez J.M., Alvarez A., Arza E.,
RA Enjuanes L.;
RT "Phosphorylation and subcellular localization of transmissible
RT gastroenteritis virus nucleocapsid protein in infected cells.";
RL J. Gen. Virol. 86:2255-2267(2005).
CC -!- FUNCTION: Packages the positive strand viral genome RNA into a helical
CC ribonucleocapsid (RNP) and plays a fundamental role during virion
CC assembly through its interactions with the viral genome and membrane
CC protein M. Plays an important role in enhancing the efficiency of
CC subgenomic viral RNA transcription as well as viral replication.
CC {ECO:0000250|UniProtKB:P03416, ECO:0000255|HAMAP-Rule:MF_04095,
CC ECO:0000269|PubMed:15507657}.
CC -!- SUBUNIT: Homooligomer. Both monomeric and oligomeric forms interact
CC with RNA. Interacts with protein M. Interacts with NSP3; this
CC interaction serves to tether the genome to the newly translated
CC replicase-transcriptase complex at a very early stage of infection.
CC {ECO:0000250|UniProtKB:P03416, ECO:0000255|HAMAP-Rule:MF_04095}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000250|UniProtKB:P03416,
CC ECO:0000255|HAMAP-Rule:MF_04095}. Host endoplasmic reticulum-Golgi
CC intermediate compartment {ECO:0000250|UniProtKB:P03416,
CC ECO:0000255|HAMAP-Rule:MF_04095}. Host Golgi apparatus
CC {ECO:0000250|UniProtKB:P03416, ECO:0000255|HAMAP-Rule:MF_04095}.
CC Note=Located inside the virion, complexed with the viral RNA. Probably
CC associates with ER-derived membranes where it participates in viral RNA
CC synthesis and virus budding. {ECO:0000250|UniProtKB:P03416,
CC ECO:0000255|HAMAP-Rule:MF_04095}.
CC -!- PTM: ADP-ribosylated. The ADP-ribosylation is retained in the virion
CC during infection. {ECO:0000250|UniProtKB:P03416, ECO:0000255|HAMAP-
CC Rule:MF_04095}.
CC -!- PTM: Phosphorylated on serine and threonine residues.
CC {ECO:0000250|UniProtKB:P03416, ECO:0000255|HAMAP-Rule:MF_04095,
CC ECO:0000269|PubMed:16033973}.
CC -!- SIMILARITY: Belongs to the alphacoronavirus nucleocapsid protein
CC family. {ECO:0000255|HAMAP-Rule:MF_04095}.
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DR EMBL; X06371; CAA29674.1; -; Genomic_RNA.
DR EMBL; M14878; AAA47915.1; -; Genomic_RNA.
DR EMBL; AJ271965; CAB91150.1; -; Genomic_RNA.
DR EMBL; M21627; AAA47913.1; -; Genomic_RNA.
DR PIR; A04025; VHIHPC.
DR SMR; P04134; -.
DR IntAct; P04134; 1.
DR iPTMnet; P04134; -.
DR Proteomes; UP000001440; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IDA:UniProtKB.
DR GO; GO:0044172; C:host cell endoplasmic reticulum-Golgi intermediate compartment; IEA:UniProtKB-SubCell.
DR GO; GO:0044177; C:host cell Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR CDD; cd21595; CoV_N-CTD; 1.
DR CDD; cd21554; CoV_N-NTD; 1.
DR HAMAP; MF_04095; ALPHA_CORONA_NCAP; 1.
DR InterPro; IPR044344; N_prot_C_CoV.
DR InterPro; IPR044345; N_prot_N_CoV.
DR InterPro; IPR042548; NCAP_aCoV.
DR InterPro; IPR001218; Nucleocap_CoV.
DR InterPro; IPR037179; Nucleocapsid_C.
DR InterPro; IPR037195; Nucleocapsid_N.
DR Pfam; PF00937; CoV_nucleocap; 1.
DR PIRSF; PIRSF003888; Corona_nucleocap; 1.
DR SUPFAM; SSF103068; SSF103068; 1.
DR SUPFAM; SSF110304; SSF110304; 1.
DR PROSITE; PS51929; COV_N_CTD; 1.
DR PROSITE; PS51928; COV_N_NTD; 1.
PE 1: Evidence at protein level;
KW ADP-ribosylation; Host Golgi apparatus; Phosphoprotein; Reference proteome;
KW Ribonucleoprotein; RNA-binding; Transcription; Transcription regulation;
KW Viral nucleoprotein; Virion.
FT CHAIN 1..382
FT /note="Nucleoprotein"
FT /id="PRO_0000106014"
FT DOMAIN 31..153
FT /note="CoV N NTD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01276"
FT DOMAIN 224..337
FT /note="CoV N CTD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01277"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 33..159
FT /note="RNA-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04095"
FT REGION 150..240
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 231..334
FT /note="Dimerization"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04095"
FT REGION 328..358
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 204..240
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 334..357
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 9
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04095,
FT ECO:0000269|PubMed:16033973"
FT MOD_RES 156
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04095,
FT ECO:0000269|PubMed:16033973"
FT MOD_RES 254
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04095,
FT ECO:0000269|PubMed:16033973"
FT MOD_RES 256
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04095,
FT ECO:0000269|PubMed:16033973"
FT CONFLICT 237
FT /note="W -> S (in Ref. 2; AAA47915)"
FT CONFLICT 376
FT /note="I -> N (in Ref. 1; CAA29674)"
SQ SEQUENCE 382 AA; 43521 MW; E299502A0FB36ABA CRC64;
MANQGQRVSW GDESTKTRGR SNSRGRKNNN IPLSFFNPIT LQQGSKFWNL CPRDFVPKGI
GNRDQQIGYW NRQTRYRMVK GQRKELPERW FFYYLGTGPH ADAKFKDKLD GVVWVAKDGA
MNKPTTLGSR GANNESKALK FDGKVPGEFQ LEVNQSRDNS RSRSQSRSRS RNRSQSRGRQ
QFNNKKDDSV EQAVLAALKK LGVDTEKQQQ RSRSKSKERS NSKTRDTTPK NENKHTWKRT
AGKGDVTRFY GARSSSANFG DTDLVANGSS AKHYPQLAEC VPSVSSILFG SYWTSKEDGD
QIEVTFTHKY HLPKDDPKTG QFLQQINAYA RPSEVAKEQR KRKSRSKSAE RSEQDVVPDA
LIENYTDVFD DTQVEIIDEV TN
